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Tracking the evolutionary loss of hemoglobin expression by the white-blooded Antarctic icefishes.
Gene. 2002 Aug 07; 295(2):185-91.GENE

Abstract

The blood of Antarctic icefishes (family Channichthyidae, suborder Notothenioidei) is completely devoid of hemoglobin. Icefishes have developed compensatory adaptations that reduce oxygen demand and enhance oxygen transport. Oxygen delivery to tissues occurs by carrying the gas physically dissolved in the plasma. To evaluate the evolutionary pathway leading to the icefish hemoglobinless phenotype, the adult and embryonic/juvenile gene complexes from a closely related, red-blooded notothenioid species were isolated and characterized. The hybridization pattern of notothenioid adult globin cDNAs showed that the genomes of three icefish species retain transcriptionally inactive alpha1-globin-related DNA sequences, which are identical truncated variants of the alpha1-globin gene of the red-blooded fish, containing part of intron 2, all of exon 3, and the 3'-untranslated region. The icefish genomes have no beta-globin genes. Furthermore, Southern blots of genomic DNA from red- and white-blooded (two species) notothenioids, probed with fragments of the genes flanking the ends of the embryonic/juvenile complex, indicated that icefishes have also lost embryonic/juvenile globin genes. It is proposed that inability to express hemoglobin arose from a single, large-scale deletional event, which removed all icefish globin genes with the exception of the 3' end of alpha1.

Authors+Show Affiliations

Institute of Protein Biochemistry and Enzymology, Consiglio Nazionale delle Ricerche,Via Marconi 12, I-80125, Naples, Italy. diprisco@dafne.ibpe.na.cnr.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Review

Language

eng

PubMed ID

12354652

Citation

di Prisco, Guido, et al. "Tracking the Evolutionary Loss of Hemoglobin Expression By the White-blooded Antarctic Icefishes." Gene, vol. 295, no. 2, 2002, pp. 185-91.
di Prisco G, Cocca E, Parker S, et al. Tracking the evolutionary loss of hemoglobin expression by the white-blooded Antarctic icefishes. Gene. 2002;295(2):185-91.
di Prisco, G., Cocca, E., Parker, S., & Detrich, H. (2002). Tracking the evolutionary loss of hemoglobin expression by the white-blooded Antarctic icefishes. Gene, 295(2), 185-91.
di Prisco G, et al. Tracking the Evolutionary Loss of Hemoglobin Expression By the White-blooded Antarctic Icefishes. Gene. 2002 Aug 7;295(2):185-91. PubMed PMID: 12354652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tracking the evolutionary loss of hemoglobin expression by the white-blooded Antarctic icefishes. AU - di Prisco,Guido, AU - Cocca,Ennio, AU - Parker,Sandra, AU - Detrich,H, PY - 2002/10/2/pubmed PY - 2003/1/23/medline PY - 2002/10/2/entrez SP - 185 EP - 91 JF - Gene JO - Gene VL - 295 IS - 2 N2 - The blood of Antarctic icefishes (family Channichthyidae, suborder Notothenioidei) is completely devoid of hemoglobin. Icefishes have developed compensatory adaptations that reduce oxygen demand and enhance oxygen transport. Oxygen delivery to tissues occurs by carrying the gas physically dissolved in the plasma. To evaluate the evolutionary pathway leading to the icefish hemoglobinless phenotype, the adult and embryonic/juvenile gene complexes from a closely related, red-blooded notothenioid species were isolated and characterized. The hybridization pattern of notothenioid adult globin cDNAs showed that the genomes of three icefish species retain transcriptionally inactive alpha1-globin-related DNA sequences, which are identical truncated variants of the alpha1-globin gene of the red-blooded fish, containing part of intron 2, all of exon 3, and the 3'-untranslated region. The icefish genomes have no beta-globin genes. Furthermore, Southern blots of genomic DNA from red- and white-blooded (two species) notothenioids, probed with fragments of the genes flanking the ends of the embryonic/juvenile complex, indicated that icefishes have also lost embryonic/juvenile globin genes. It is proposed that inability to express hemoglobin arose from a single, large-scale deletional event, which removed all icefish globin genes with the exception of the 3' end of alpha1. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/12354652/Tracking_the_evolutionary_loss_of_hemoglobin_expression_by_the_white_blooded_Antarctic_icefishes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378111902006911 DB - PRIME DP - Unbound Medicine ER -