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Passive stiffness changes in soleus muscles from desmin knockout mice are not due to titin modifications.

Abstract

Passive stiffness was found to be increased in mouse soleus muscles lacking desmin. Because titin is considered to be the major source of muscle elasticity, the stiffening might be explainable by titin adaptation. To test this, passive mechanical properties of single skinned fibres of soleus muscles from desmin knockout and control mice were analysed by using various extension tests. Titin expression was studied by SDS-gel electrophoresis. Absence of desmin did not modify either electrophoretic mobility of the titin band (3700 kDa) or optical density-unit ratios between bands for titin and nebulin (congruent with 0.3) and bands for titin and myosin heavy chain (congruent with 0.08). Elastic properties of fibres were not altered in the absence of desmin since passive tensions were similar under quasi-static (56-66 kN m(-2)) and dynamic (100-118 kN m(-2)) conditions whatever the kind of fibre. Thus, titin is unlikely to be responsible for the large increase in passive stiffness observed in whole soleus muscles when desmin is lacking.

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  • Authors+Show Affiliations

    ,

    Unité de Biomécanique et Génie Biomédical, CNRS-UMR 6600, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex, France.

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    Source

    MeSH

    Animals
    Connectin
    Desmin
    Elasticity
    Electrophoresis, Polyacrylamide Gel
    Mice
    Mice, Knockout
    Muscle Contraction
    Muscle Fibers, Fast-Twitch
    Muscle Fibers, Slow-Twitch
    Muscle Proteins
    Muscle, Skeletal
    Myosin Heavy Chains
    Protein Kinases

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    12355177

    Citation

    Anderson, J, et al. "Passive Stiffness Changes in Soleus Muscles From Desmin Knockout Mice Are Not Due to Titin Modifications." Pflugers Archiv : European Journal of Physiology, vol. 444, no. 6, 2002, pp. 771-6.
    Anderson J, Joumaa V, Stevens L, et al. Passive stiffness changes in soleus muscles from desmin knockout mice are not due to titin modifications. Pflugers Arch. 2002;444(6):771-6.
    Anderson, J., Joumaa, V., Stevens, L., Neagoe, C., Li, Z., Mounier, Y., ... Goubel, F. (2002). Passive stiffness changes in soleus muscles from desmin knockout mice are not due to titin modifications. Pflugers Archiv : European Journal of Physiology, 444(6), pp. 771-6.
    Anderson J, et al. Passive Stiffness Changes in Soleus Muscles From Desmin Knockout Mice Are Not Due to Titin Modifications. Pflugers Arch. 2002;444(6):771-6. PubMed PMID: 12355177.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Passive stiffness changes in soleus muscles from desmin knockout mice are not due to titin modifications. AU - Anderson,J, AU - Joumaa,V, AU - Stevens,L, AU - Neagoe,C, AU - Li,Z, AU - Mounier,Y, AU - Linke,W A, AU - Goubel,F, Y1 - 2002/06/22/ PY - 2002/04/04/received PY - 2002/05/08/revised PY - 2002/10/2/pubmed PY - 2003/4/24/medline PY - 2002/10/2/entrez SP - 771 EP - 6 JF - Pflugers Archiv : European journal of physiology JO - Pflugers Arch. VL - 444 IS - 6 N2 - Passive stiffness was found to be increased in mouse soleus muscles lacking desmin. Because titin is considered to be the major source of muscle elasticity, the stiffening might be explainable by titin adaptation. To test this, passive mechanical properties of single skinned fibres of soleus muscles from desmin knockout and control mice were analysed by using various extension tests. Titin expression was studied by SDS-gel electrophoresis. Absence of desmin did not modify either electrophoretic mobility of the titin band (3700 kDa) or optical density-unit ratios between bands for titin and nebulin (congruent with 0.3) and bands for titin and myosin heavy chain (congruent with 0.08). Elastic properties of fibres were not altered in the absence of desmin since passive tensions were similar under quasi-static (56-66 kN m(-2)) and dynamic (100-118 kN m(-2)) conditions whatever the kind of fibre. Thus, titin is unlikely to be responsible for the large increase in passive stiffness observed in whole soleus muscles when desmin is lacking. SN - 0031-6768 UR - https://www.unboundmedicine.com/medline/citation/12355177/Passive_stiffness_changes_in_soleus_muscles_from_desmin_knockout_mice_are_not_due_to_titin_modifications_ L2 - https://dx.doi.org/10.1007/s00424-002-0875-0 DB - PRIME DP - Unbound Medicine ER -