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2,2'-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls.
J Agric Food Chem. 2002 Oct 09; 50(21):5850-5.JA

Abstract

An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods ((1)H NMR, (13)C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).

Authors+Show Affiliations

Kuo CC
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan, Republic of China.
Chiang W
No affiliation info available
Liu GP
No affiliation info available
Chien YL
No affiliation info available
Chang JY
No affiliation info available
Lee CK
No affiliation info available
Lo JM
No affiliation info available
Huang SL
No affiliation info available
Shih MC
No affiliation info available
Kuo YH
No affiliation info available

MeSH

AntioxidantsBiphenyl CompoundsChromatography, High Pressure LiquidFree Radical ScavengersMagnetic Resonance SpectroscopyMethanolPicratesPlant ExtractsPlant StructuresPoaceaeSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12358449

Citation

Kuo, Ching-Chuan, et al. "2,2'-Diphenyl-1-picrylhydrazyl Radical-scavenging Active Components From Adlay (Coix Lachryma-jobi L. Var. Ma-yuen Stapf) Hulls." Journal of Agricultural and Food Chemistry, vol. 50, no. 21, 2002, pp. 5850-5.
Kuo CC, Chiang W, Liu GP, et al. 2,2'-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls. J Agric Food Chem. 2002;50(21):5850-5.
Kuo, C. C., Chiang, W., Liu, G. P., Chien, Y. L., Chang, J. Y., Lee, C. K., Lo, J. M., Huang, S. L., Shih, M. C., & Kuo, Y. H. (2002). 2,2'-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls. Journal of Agricultural and Food Chemistry, 50(21), 5850-5.
Kuo CC, et al. 2,2'-Diphenyl-1-picrylhydrazyl Radical-scavenging Active Components From Adlay (Coix Lachryma-jobi L. Var. Ma-yuen Stapf) Hulls. J Agric Food Chem. 2002 Oct 9;50(21):5850-5. PubMed PMID: 12358449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 2,2'-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls. AU - Kuo,Ching-Chuan, AU - Chiang,Wenchang, AU - Liu,Guey-Ping, AU - Chien,Ya-Lin, AU - Chang,Jang-Yang, AU - Lee,Ching-Kuo, AU - Lo,Jir-Mehng, AU - Huang,Shou-Ling, AU - Shih,Ming-Chih, AU - Kuo,Yueh-Hsiung, PY - 2002/10/3/pubmed PY - 2002/11/26/medline PY - 2002/10/3/entrez SP - 5850 EP - 5 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 50 IS - 21 N2 - An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods ((1)H NMR, (13)C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6). SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12358449/22'_Diphenyl_1_picrylhydrazyl_radical_scavenging_active_components_from_adlay__Coix_lachryma_jobi_L__var__ma_yuen_Stapf__hulls_ DB - PRIME DP - Unbound Medicine ER -
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