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Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients.
Ophthalmology. 2002 Oct; 109(10):1780-7.O

Abstract

PURPOSE

Dietary carotenoids lutein and zeaxanthin may play a protective role against visual loss from age-related macular degeneration (AMD) through antioxidant and light screening mechanisms. We used a novel noninvasive objective method to quantify lutein and zeaxanthin in the human macula using resonance Raman spectroscopy and compared macular pigment levels in AMD and normal subjects.

DESIGN

Observational study of an ophthalmology clinic-based population.

PARTICIPANTS AND CONTROLS

Ninety-three AMD eyes from 63 patients and 220 normal eyes from 138 subjects.

METHODS

Macular carotenoid levels were quantified by illuminating the macula with a low-power argon laser spot and measuring Raman backscattered light using a spectrograph. This technique is sensitive, specific, and repeatable even in subjects with significant macular pathologic features.

MAIN OUTCOME MEASURE

Raman signal intensity at 1525 cm(-1) generated by the carbon-carbon double-bond vibrations of lutein and zeaxanthin.

RESULTS

Carotenoid Raman signal intensity declined with age in normal eyes (P < 0.001). Average levels of lutein and zeaxanthin were 32% lower in AMD eyes versus normal elderly control eyes as long as the subjects were not consuming high-dose lutein supplements (P = 0.001). Patients who had begun to consume supplements containing high doses of lutein (> or =4 mg/day) regularly after their initial diagnosis of AMD had average macular pigment levels that were in the normal range (P = 0.829) and that were significantly higher than in AMD patients not consuming these supplements (P = 0.038).

CONCLUSIONS

These findings are consistent with the hypothesis that low levels of lutein and zeaxanthin in the human macula may represent a pathogenic risk factor for the development of AMD. Resonance Raman measurement of macular carotenoid pigments could play an important role in facilitating large-scale prospective clinical studies of lutein and zeaxanthin protection against AMD, and this technology may someday prove useful in the early detection of individuals at risk for visual loss from AMD.

Authors+Show Affiliations

Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA. paul.bernsteinhsc@hsc.utah.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12359594

Citation

Bernstein, Paul S., et al. "Resonance Raman Measurement of Macular Carotenoids in Normal Subjects and in Age-related Macular Degeneration Patients." Ophthalmology, vol. 109, no. 10, 2002, pp. 1780-7.
Bernstein PS, Zhao DY, Wintch SW, et al. Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients. Ophthalmology. 2002;109(10):1780-7.
Bernstein, P. S., Zhao, D. Y., Wintch, S. W., Ermakov, I. V., McClane, R. W., & Gellermann, W. (2002). Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients. Ophthalmology, 109(10), 1780-7.
Bernstein PS, et al. Resonance Raman Measurement of Macular Carotenoids in Normal Subjects and in Age-related Macular Degeneration Patients. Ophthalmology. 2002;109(10):1780-7. PubMed PMID: 12359594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients. AU - Bernstein,Paul S, AU - Zhao,Da-You, AU - Wintch,Steven W, AU - Ermakov,Igor V, AU - McClane,Robert W, AU - Gellermann,Werner, PY - 2002/10/3/pubmed PY - 2002/11/26/medline PY - 2002/10/3/entrez SP - 1780 EP - 7 JF - Ophthalmology JO - Ophthalmology VL - 109 IS - 10 N2 - PURPOSE: Dietary carotenoids lutein and zeaxanthin may play a protective role against visual loss from age-related macular degeneration (AMD) through antioxidant and light screening mechanisms. We used a novel noninvasive objective method to quantify lutein and zeaxanthin in the human macula using resonance Raman spectroscopy and compared macular pigment levels in AMD and normal subjects. DESIGN: Observational study of an ophthalmology clinic-based population. PARTICIPANTS AND CONTROLS: Ninety-three AMD eyes from 63 patients and 220 normal eyes from 138 subjects. METHODS: Macular carotenoid levels were quantified by illuminating the macula with a low-power argon laser spot and measuring Raman backscattered light using a spectrograph. This technique is sensitive, specific, and repeatable even in subjects with significant macular pathologic features. MAIN OUTCOME MEASURE: Raman signal intensity at 1525 cm(-1) generated by the carbon-carbon double-bond vibrations of lutein and zeaxanthin. RESULTS: Carotenoid Raman signal intensity declined with age in normal eyes (P < 0.001). Average levels of lutein and zeaxanthin were 32% lower in AMD eyes versus normal elderly control eyes as long as the subjects were not consuming high-dose lutein supplements (P = 0.001). Patients who had begun to consume supplements containing high doses of lutein (> or =4 mg/day) regularly after their initial diagnosis of AMD had average macular pigment levels that were in the normal range (P = 0.829) and that were significantly higher than in AMD patients not consuming these supplements (P = 0.038). CONCLUSIONS: These findings are consistent with the hypothesis that low levels of lutein and zeaxanthin in the human macula may represent a pathogenic risk factor for the development of AMD. Resonance Raman measurement of macular carotenoid pigments could play an important role in facilitating large-scale prospective clinical studies of lutein and zeaxanthin protection against AMD, and this technology may someday prove useful in the early detection of individuals at risk for visual loss from AMD. SN - 0161-6420 UR - https://www.unboundmedicine.com/medline/citation/12359594/Resonance_Raman_measurement_of_macular_carotenoids_in_normal_subjects_and_in_age_related_macular_degeneration_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-6420(02)01173-9 DB - PRIME DP - Unbound Medicine ER -