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Stabilization of gelatin films by crosslinking with genipin.
Biomaterials. 2002 Dec; 23(24):4827-32.B

Abstract

The possibility to stabilize gelatin films by crosslinking with genipin was investigated through a mechanical, chemical and thermal characterization of samples treated with genipin solutions at different concentrations. The extent of crosslinking, evaluated as difference between the number of free epsilon -amino groups before and after crosslinking, increases as a function of genipin concentration up to about 85%. Simultaneously, the deformability of the films decreases whereas the Young's modulus E, increases. Furthermore, crosslinking provokes a significant reduction of the swelling in physiological solution, and enhances the thermal stability of the samples, as indicated by the results of the d.s.c. investigation. The data obtained from the films treated with genipin at concentrations higher than 0.67% are quite similar, and indicative of a good stabilizing effect of genipin. In spite of the small gelatin release (2%) observed after 1 month of storage in buffer solution, the mechanical, thermal and swelling properties of the films are very close to those previously obtained for glutaraldehyde crosslinked gelatin, and suggest that genipin, which is by far less cytotoxic, can be considered a valid alternative for crosslinking gelatin biomaterials.

Authors+Show Affiliations

Department of Chemistry G. Ciamician, University of Bologna, 40126 Bologna, Italy. bigi@ciam.unibo.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12361622

Citation

Bigi, A, et al. "Stabilization of Gelatin Films By Crosslinking With Genipin." Biomaterials, vol. 23, no. 24, 2002, pp. 4827-32.
Bigi A, Cojazzi G, Panzavolta S, et al. Stabilization of gelatin films by crosslinking with genipin. Biomaterials. 2002;23(24):4827-32.
Bigi, A., Cojazzi, G., Panzavolta, S., Roveri, N., & Rubini, K. (2002). Stabilization of gelatin films by crosslinking with genipin. Biomaterials, 23(24), 4827-32.
Bigi A, et al. Stabilization of Gelatin Films By Crosslinking With Genipin. Biomaterials. 2002;23(24):4827-32. PubMed PMID: 12361622.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stabilization of gelatin films by crosslinking with genipin. AU - Bigi,A, AU - Cojazzi,G, AU - Panzavolta,S, AU - Roveri,N, AU - Rubini,K, PY - 2002/10/4/pubmed PY - 2003/3/26/medline PY - 2002/10/4/entrez SP - 4827 EP - 32 JF - Biomaterials JO - Biomaterials VL - 23 IS - 24 N2 - The possibility to stabilize gelatin films by crosslinking with genipin was investigated through a mechanical, chemical and thermal characterization of samples treated with genipin solutions at different concentrations. The extent of crosslinking, evaluated as difference between the number of free epsilon -amino groups before and after crosslinking, increases as a function of genipin concentration up to about 85%. Simultaneously, the deformability of the films decreases whereas the Young's modulus E, increases. Furthermore, crosslinking provokes a significant reduction of the swelling in physiological solution, and enhances the thermal stability of the samples, as indicated by the results of the d.s.c. investigation. The data obtained from the films treated with genipin at concentrations higher than 0.67% are quite similar, and indicative of a good stabilizing effect of genipin. In spite of the small gelatin release (2%) observed after 1 month of storage in buffer solution, the mechanical, thermal and swelling properties of the films are very close to those previously obtained for glutaraldehyde crosslinked gelatin, and suggest that genipin, which is by far less cytotoxic, can be considered a valid alternative for crosslinking gelatin biomaterials. SN - 0142-9612 UR - https://www.unboundmedicine.com/medline/citation/12361622/Stabilization_of_gelatin_films_by_crosslinking_with_genipin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0142961202002351 DB - PRIME DP - Unbound Medicine ER -