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Gene expression and immunohistochemical localization of osteonectin in association with early bone formation in the developing mandible.
Histochem J 2002 Jan-Feb; 34(1-2):57-66HJ

Abstract

We have compared the expression of osteonectin with that of osteocalcin and bone sialoprotein during bone formation in the rat mandible, using in situ hybridization and immunohistochemistry. Expression of osteonectin, osteocalcin and bone sialoprotein mRNAs were first observed in newly differentiated osteoblasts of the developing mandible at embryonic day 15 (E15) and subsequently increased with the number of osteoblasts through E20. Definitive osteonectin immunostaining was observed in newly differentiated osteoblasts, but not in the intercellular unmineralized matrix. Immunostaining for osteocalcin and bone sialoprotein was visible in osteoblasts and unmineralized matrix. Concomitant with the initiation of matrix mineralization at E16, mineralized bone matrix showed osteocalcin and bone sialoprotein immunostaining, but lacked osteonectin immunostaining. The same staining profile was observed during subsequent phases of bone formation at E17-20. However, sequential demineralization with ethanolic trimethylammonium EDTA and protease digestion of tissue sections demonstrated prominent osteonectin immunostaining of the mineralized bone matrix. Western blot analysis of osteonectin in extracts of fresh specimens at E18 and 20 revealed that an EDTA extract contains osteonectin having M, approximately 50 kDa. These results indicate that newly differentiated osteoblasts synthesize and secrete osteonectin, which is mainly incorporated into the mineralized bone matrix and becomes a specific component of developing manibula of foetal rats.

Authors+Show Affiliations

Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12365801

Citation

Ishigaki, Ryo, et al. "Gene Expression and Immunohistochemical Localization of Osteonectin in Association With Early Bone Formation in the Developing Mandible." The Histochemical Journal, vol. 34, no. 1-2, 2002, pp. 57-66.
Ishigaki R, Takagi M, Igarashi M, et al. Gene expression and immunohistochemical localization of osteonectin in association with early bone formation in the developing mandible. Histochem J. 2002;34(1-2):57-66.
Ishigaki, R., Takagi, M., Igarashi, M., & Ito, K. (2002). Gene expression and immunohistochemical localization of osteonectin in association with early bone formation in the developing mandible. The Histochemical Journal, 34(1-2), pp. 57-66.
Ishigaki R, et al. Gene Expression and Immunohistochemical Localization of Osteonectin in Association With Early Bone Formation in the Developing Mandible. Histochem J. 2002;34(1-2):57-66. PubMed PMID: 12365801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression and immunohistochemical localization of osteonectin in association with early bone formation in the developing mandible. AU - Ishigaki,Ryo, AU - Takagi,Minoru, AU - Igarashi,Masato, AU - Ito,Koichi, PY - 2002/10/9/pubmed PY - 2003/4/4/medline PY - 2002/10/9/entrez SP - 57 EP - 66 JF - The Histochemical journal JO - Histochem. J. VL - 34 IS - 1-2 N2 - We have compared the expression of osteonectin with that of osteocalcin and bone sialoprotein during bone formation in the rat mandible, using in situ hybridization and immunohistochemistry. Expression of osteonectin, osteocalcin and bone sialoprotein mRNAs were first observed in newly differentiated osteoblasts of the developing mandible at embryonic day 15 (E15) and subsequently increased with the number of osteoblasts through E20. Definitive osteonectin immunostaining was observed in newly differentiated osteoblasts, but not in the intercellular unmineralized matrix. Immunostaining for osteocalcin and bone sialoprotein was visible in osteoblasts and unmineralized matrix. Concomitant with the initiation of matrix mineralization at E16, mineralized bone matrix showed osteocalcin and bone sialoprotein immunostaining, but lacked osteonectin immunostaining. The same staining profile was observed during subsequent phases of bone formation at E17-20. However, sequential demineralization with ethanolic trimethylammonium EDTA and protease digestion of tissue sections demonstrated prominent osteonectin immunostaining of the mineralized bone matrix. Western blot analysis of osteonectin in extracts of fresh specimens at E18 and 20 revealed that an EDTA extract contains osteonectin having M, approximately 50 kDa. These results indicate that newly differentiated osteoblasts synthesize and secrete osteonectin, which is mainly incorporated into the mineralized bone matrix and becomes a specific component of developing manibula of foetal rats. SN - 0018-2214 UR - https://www.unboundmedicine.com/medline/citation/12365801/Gene_expression_and_immunohistochemical_localization_of_osteonectin_in_association_with_early_bone_formation_in_the_developing_mandible_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=12365801.ui DB - PRIME DP - Unbound Medicine ER -