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Analyzing red blood cell-deformability distributions.
Blood Cells Mol Dis. 2002 May-Jun; 28(3):373-84.BC

Abstract

BACKGROUND

Red blood cells (RBCs) must deform to pass the smallest capillaries of the microcirculation. Available techniques for measuring RBC deformability often provide an indication of the mean deformability. The latter may be decreased either by a slight overall deformability reduction or by the presence of a small fraction of rigid cells. A distinction between these two cases can be made with a RBC-deformability distribution (RBC-DD).

METHODS

This paper explores RBC-DDs of healthy individuals and of cells with anomalous mechanical properties (sickle cell disease, dialysis patients, elliptocytosis, and cultivated malaria tropica). The distributions were measured with an automated rheoscope, which uses advanced image analysis techniques to obtain the deformability index of a large number of individual cells subjected to simple shear flow.

RESULTS

The RBC-DD of healthy volunteers is close to a normal distribution. In the investigated patients, distributions were markedly different and yielded significant changes in the mean, in the standard deviation, or in both. The presence of hypodeformable and hyperdeformable cell fractions can qualitatively and quantitatively be assessed from the deformability distribution (DD). In elliptocytosis, cells orient differently with respect to the streamlines, compared to normal cells. This causes the DD to be biased.

CONCLUSIONS

The RBC-DD is a powerful representation to establish subpopulations with anomalous deformability. Fractions of hypodeformable and hyperdeformable cells and the standard deviation of the DD are new and excellent quantitative parameters to assess alterations in RBC deformability.

Authors+Show Affiliations

Dobbe JG
Department of Medical Technological Development, Academic Medical Center, University of Amsterdam, The Netherlands. j.g.dobbe@amc.uva.nl
Hardeman MR
No affiliation info available
Streekstra GJ
No affiliation info available
Strackee J
No affiliation info available
Ince C
No affiliation info available
Grimbergen CA
No affiliation info available

MeSH

Anemia, Sickle CellCase-Control StudiesElliptocytosis, HereditaryEquipment DesignErythrocyte DeformabilityErythrocytes, AbnormalErythropoietinFemaleHematologic TestsHumansMalariaMaleMicroscopy, VideoPerfusionRenal DialysisStatistical DistributionsStress, Mechanical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12367581

Citation

Dobbe, J G G., et al. "Analyzing Red Blood Cell-deformability Distributions." Blood Cells, Molecules & Diseases, vol. 28, no. 3, 2002, pp. 373-84.
Dobbe JG, Hardeman MR, Streekstra GJ, et al. Analyzing red blood cell-deformability distributions. Blood Cells Mol Dis. 2002;28(3):373-84.
Dobbe, J. G., Hardeman, M. R., Streekstra, G. J., Strackee, J., Ince, C., & Grimbergen, C. A. (2002). Analyzing red blood cell-deformability distributions. Blood Cells, Molecules & Diseases, 28(3), 373-84.
Dobbe JG, et al. Analyzing Red Blood Cell-deformability Distributions. Blood Cells Mol Dis. 2002 May-Jun;28(3):373-84. PubMed PMID: 12367581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analyzing red blood cell-deformability distributions. AU - Dobbe,J G G, AU - Hardeman,M R, AU - Streekstra,G J, AU - Strackee,J, AU - Ince,C, AU - Grimbergen,C A, PY - 2002/10/9/pubmed PY - 2003/7/15/medline PY - 2002/10/9/entrez SP - 373 EP - 84 JF - Blood cells, molecules & diseases JO - Blood Cells Mol Dis VL - 28 IS - 3 N2 - BACKGROUND: Red blood cells (RBCs) must deform to pass the smallest capillaries of the microcirculation. Available techniques for measuring RBC deformability often provide an indication of the mean deformability. The latter may be decreased either by a slight overall deformability reduction or by the presence of a small fraction of rigid cells. A distinction between these two cases can be made with a RBC-deformability distribution (RBC-DD). METHODS: This paper explores RBC-DDs of healthy individuals and of cells with anomalous mechanical properties (sickle cell disease, dialysis patients, elliptocytosis, and cultivated malaria tropica). The distributions were measured with an automated rheoscope, which uses advanced image analysis techniques to obtain the deformability index of a large number of individual cells subjected to simple shear flow. RESULTS: The RBC-DD of healthy volunteers is close to a normal distribution. In the investigated patients, distributions were markedly different and yielded significant changes in the mean, in the standard deviation, or in both. The presence of hypodeformable and hyperdeformable cell fractions can qualitatively and quantitatively be assessed from the deformability distribution (DD). In elliptocytosis, cells orient differently with respect to the streamlines, compared to normal cells. This causes the DD to be biased. CONCLUSIONS: The RBC-DD is a powerful representation to establish subpopulations with anomalous deformability. Fractions of hypodeformable and hyperdeformable cells and the standard deviation of the DD are new and excellent quantitative parameters to assess alterations in RBC deformability. SN - 1079-9796 UR - https://www.unboundmedicine.com/medline/citation/12367581/Analyzing_red_blood_cell_deformability_distributions_ DB - PRIME DP - Unbound Medicine ER -