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Dietary CLA alters yolk and tissue FA composition and hepatic histopathology of laying hens.
Lipids. 2002 Aug; 37(8):751-7.L

Abstract

The effect of dietary CLA along with n-3 PUFA on yolk FA profile and hepatic lipid accumulation was investigated. Laying hens (n = 40) were randomly assigned to four experimental diets containing 0, 0.5, 1.0, or 2.0% CLA. Menhaden oil was used as the source of n-3 PUFA. Dietary CLA did not affect the total lipid content of egg yolk (P > 0.05). The amounts of CLA isomers (cis-9 trans-11, trans-10 cis-12) in the egg yolk were proportional to the levels of CLA in the diet (P < 0.05). The total CLA content in the egg yolk was 0, 0.97, 2.4, and 5.3 wt%, respectively (P < 0.05). Addition of CLA resulted in an increase in saturated FA (P < 0.05) with a concomitant reduction in monounsaturated FA (P < 0.05) in the yolk, liver, abdominal fat, breast, and thigh muscle. No difference in saturated and monounsaturated FA content in heart and spleen tissue was noted. Dietary CLA at all concentrations resulted in an increase (P < 0.05) in the total number of fat vacuoles and lipid infiltration in hepatocytes. The number of cells with 75% or higher lipid vacuolation in the cytoplasm was also increased (P < 0.05) by 2.0% CLA. Dietary CLA at 0.5% levels resulted in an increase (P < 0.05) in the total lipid content of hepatic tissue. The total lipid content in leg muscle was lower (P < 0.05) in CLA-fed birds. However, no effect of CLA on lipid content of breast muscle, heart, spleen and adipose tissue was observed (P > 0.05). The current study used CLA in a FFA form. The effects of using CLA in other form such as TG on avian hepatic tissue need to be investigated.

Authors+Show Affiliations

Department of Animal Sciences, Oregon State University, Corvallis 97331, USA. Gita.Cherian@orst.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12371745

Citation

Cherian, Gita, et al. "Dietary CLA Alters Yolk and Tissue FA Composition and Hepatic Histopathology of Laying Hens." Lipids, vol. 37, no. 8, 2002, pp. 751-7.
Cherian G, Holsonbake TB, Goeger MP, et al. Dietary CLA alters yolk and tissue FA composition and hepatic histopathology of laying hens. Lipids. 2002;37(8):751-7.
Cherian, G., Holsonbake, T. B., Goeger, M. P., & Bildfell, R. (2002). Dietary CLA alters yolk and tissue FA composition and hepatic histopathology of laying hens. Lipids, 37(8), 751-7.
Cherian G, et al. Dietary CLA Alters Yolk and Tissue FA Composition and Hepatic Histopathology of Laying Hens. Lipids. 2002;37(8):751-7. PubMed PMID: 12371745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary CLA alters yolk and tissue FA composition and hepatic histopathology of laying hens. AU - Cherian,Gita, AU - Holsonbake,Troy B, AU - Goeger,Mary P, AU - Bildfell,Rob, PY - 2002/10/10/pubmed PY - 2003/4/9/medline PY - 2002/10/10/entrez SP - 751 EP - 7 JF - Lipids JO - Lipids VL - 37 IS - 8 N2 - The effect of dietary CLA along with n-3 PUFA on yolk FA profile and hepatic lipid accumulation was investigated. Laying hens (n = 40) were randomly assigned to four experimental diets containing 0, 0.5, 1.0, or 2.0% CLA. Menhaden oil was used as the source of n-3 PUFA. Dietary CLA did not affect the total lipid content of egg yolk (P > 0.05). The amounts of CLA isomers (cis-9 trans-11, trans-10 cis-12) in the egg yolk were proportional to the levels of CLA in the diet (P < 0.05). The total CLA content in the egg yolk was 0, 0.97, 2.4, and 5.3 wt%, respectively (P < 0.05). Addition of CLA resulted in an increase in saturated FA (P < 0.05) with a concomitant reduction in monounsaturated FA (P < 0.05) in the yolk, liver, abdominal fat, breast, and thigh muscle. No difference in saturated and monounsaturated FA content in heart and spleen tissue was noted. Dietary CLA at all concentrations resulted in an increase (P < 0.05) in the total number of fat vacuoles and lipid infiltration in hepatocytes. The number of cells with 75% or higher lipid vacuolation in the cytoplasm was also increased (P < 0.05) by 2.0% CLA. Dietary CLA at 0.5% levels resulted in an increase (P < 0.05) in the total lipid content of hepatic tissue. The total lipid content in leg muscle was lower (P < 0.05) in CLA-fed birds. However, no effect of CLA on lipid content of breast muscle, heart, spleen and adipose tissue was observed (P > 0.05). The current study used CLA in a FFA form. The effects of using CLA in other form such as TG on avian hepatic tissue need to be investigated. SN - 0024-4201 UR - https://www.unboundmedicine.com/medline/citation/12371745/Dietary_CLA_alters_yolk_and_tissue_FA_composition_and_hepatic_histopathology_of_laying_hens_ L2 - https://link.springer.com/article/10.1007/s11745-002-0957-4 DB - PRIME DP - Unbound Medicine ER -