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Prospective assessment of a new polymerase chain reaction target (STEVOR) for imported Plasmodium falciparum malaria.
Eur J Clin Microbiol Infect Dis. 2002 Sep; 21(9):679-81.EJ

Abstract

The diagnostic value of a polymerase chain reaction (PCR)-based method for amplifying a new target of repeated genes (STEVOR) in Plasmodium falciparum was prospectively assessed on samples from 210 febrile patients returning from areas endemic for malaria. This method is capable of detecting 0.01 parasites in one microliter of blood. Plasmodium falciparum STEVOR PCR confirmed the results of the thin- and thick-film direct examination method but identified Plasmodium falciparum in four patients in whom direct examination was inconclusive at the species level. Moreover, PCR was positive in two patients with a negative direct examination. Thus, Plasmodium falciparum STEVOR PCR had 100% sensitivity and specificity and could be used in selected parasitology laboratories when expert advice is required.

Authors+Show Affiliations

Institut de Parasitologie et de Pathologie Tropicale, 3 rue Koeberlé, 67000 Strasbourg, France. denis.filisetti@medecine.u-strasbg.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12373502

Citation

Filisetti, D, et al. "Prospective Assessment of a New Polymerase Chain Reaction Target (STEVOR) for Imported Plasmodium Falciparum Malaria." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 21, no. 9, 2002, pp. 679-81.
Filisetti D, Bombard S, N'Guiri C, et al. Prospective assessment of a new polymerase chain reaction target (STEVOR) for imported Plasmodium falciparum malaria. Eur J Clin Microbiol Infect Dis. 2002;21(9):679-81.
Filisetti, D., Bombard, S., N'Guiri, C., Dahan, R., Molet, B., Abou-Bacar, A., Hansmann, Y., Christmann, D., & Candolfi, E. (2002). Prospective assessment of a new polymerase chain reaction target (STEVOR) for imported Plasmodium falciparum malaria. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 21(9), 679-81.
Filisetti D, et al. Prospective Assessment of a New Polymerase Chain Reaction Target (STEVOR) for Imported Plasmodium Falciparum Malaria. Eur J Clin Microbiol Infect Dis. 2002;21(9):679-81. PubMed PMID: 12373502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prospective assessment of a new polymerase chain reaction target (STEVOR) for imported Plasmodium falciparum malaria. AU - Filisetti,D, AU - Bombard,S, AU - N'Guiri,C, AU - Dahan,R, AU - Molet,B, AU - Abou-Bacar,A, AU - Hansmann,Y, AU - Christmann,D, AU - Candolfi,E, Y1 - 2002/09/06/ PY - 2002/10/10/pubmed PY - 2002/11/30/medline PY - 2002/10/10/entrez SP - 679 EP - 81 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur J Clin Microbiol Infect Dis VL - 21 IS - 9 N2 - The diagnostic value of a polymerase chain reaction (PCR)-based method for amplifying a new target of repeated genes (STEVOR) in Plasmodium falciparum was prospectively assessed on samples from 210 febrile patients returning from areas endemic for malaria. This method is capable of detecting 0.01 parasites in one microliter of blood. Plasmodium falciparum STEVOR PCR confirmed the results of the thin- and thick-film direct examination method but identified Plasmodium falciparum in four patients in whom direct examination was inconclusive at the species level. Moreover, PCR was positive in two patients with a negative direct examination. Thus, Plasmodium falciparum STEVOR PCR had 100% sensitivity and specificity and could be used in selected parasitology laboratories when expert advice is required. SN - 0934-9723 UR - https://www.unboundmedicine.com/medline/citation/12373502/Prospective_assessment_of_a_new_polymerase_chain_reaction_target__STEVOR__for_imported_Plasmodium_falciparum_malaria_ DB - PRIME DP - Unbound Medicine ER -