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Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study.
Electrophoresis. 2002 Sep; 23(19):3434-44.E

Abstract

We used two-dimensional (2-D) electrophoresis to analyze the protein composition of fluid recovered by bronchoalveolar lavage (BALF) from patients with sarcoidosis and idiopathic pulmonary fibrosis, two forms of interstitial lung disease with different cellular composition and cytokine profile in BALF. They are also characterized by different pathogenesis and clinical evolution, idiopathic pulmonary fibrosis being less favorable than sarcoidosis due to rapidly progressive pulmonary fibrosis. Thirty-eight proteins or protein fragments, never previously assigned in BALF samples, were identified by various methods including mass fingerprinting of tryptic digests. Comparison of the BALF protein maps of the two groups of patients showed 32 spots with statistically significant disease-related variations in relative abundance. In sarcoidosis we found an increase in the amount of several plasma proteins, while in idiopathic pulmonary fibrosis we observed a statistically significant increase in low-molecular-weight proteins, many of which are involved in inflammatory processes (such as MIF and calgranulin) or antioxidant response (such as antioxidant peroxysomal enzyme and thioredoxin peroxidase 2). 2-D electrophoresis allowed us to identify new BALF proteins and to characterize protein composition in patients with sarcoidosis and idiophatic pulmonary fibrosis. Comparison of the gels of the two diseases showed that they differ in BALF protein profiles as they do in type of immune response.

Authors+Show Affiliations

Department of Molecular Biology, University of Siena, Italy. magi@unisi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12373774

Citation

Magi, Barbara, et al. "Bronchoalveolar Lavage Fluid Protein Composition in Patients With Sarcoidosis and Idiopathic Pulmonary Fibrosis: a Two-dimensional Electrophoretic Study." Electrophoresis, vol. 23, no. 19, 2002, pp. 3434-44.
Magi B, Bini L, Perari MG, et al. Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study. Electrophoresis. 2002;23(19):3434-44.
Magi, B., Bini, L., Perari, M. G., Fossi, A., Sanchez, J. C., Hochstrasser, D., Paesano, S., Raggiaschi, R., Santucci, A., Pallini, V., & Rottoli, P. (2002). Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study. Electrophoresis, 23(19), 3434-44.
Magi B, et al. Bronchoalveolar Lavage Fluid Protein Composition in Patients With Sarcoidosis and Idiopathic Pulmonary Fibrosis: a Two-dimensional Electrophoretic Study. Electrophoresis. 2002;23(19):3434-44. PubMed PMID: 12373774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study. AU - Magi,Barbara, AU - Bini,Luca, AU - Perari,Maria Grazia, AU - Fossi,Antonella, AU - Sanchez,Jean-Charles, AU - Hochstrasser,Denis, AU - Paesano,Salvo, AU - Raggiaschi,Roberto, AU - Santucci,Annalisa, AU - Pallini,Vitaliano, AU - Rottoli,Paola, PY - 2002/10/10/pubmed PY - 2003/6/6/medline PY - 2002/10/10/entrez SP - 3434 EP - 44 JF - Electrophoresis JO - Electrophoresis VL - 23 IS - 19 N2 - We used two-dimensional (2-D) electrophoresis to analyze the protein composition of fluid recovered by bronchoalveolar lavage (BALF) from patients with sarcoidosis and idiopathic pulmonary fibrosis, two forms of interstitial lung disease with different cellular composition and cytokine profile in BALF. They are also characterized by different pathogenesis and clinical evolution, idiopathic pulmonary fibrosis being less favorable than sarcoidosis due to rapidly progressive pulmonary fibrosis. Thirty-eight proteins or protein fragments, never previously assigned in BALF samples, were identified by various methods including mass fingerprinting of tryptic digests. Comparison of the BALF protein maps of the two groups of patients showed 32 spots with statistically significant disease-related variations in relative abundance. In sarcoidosis we found an increase in the amount of several plasma proteins, while in idiopathic pulmonary fibrosis we observed a statistically significant increase in low-molecular-weight proteins, many of which are involved in inflammatory processes (such as MIF and calgranulin) or antioxidant response (such as antioxidant peroxysomal enzyme and thioredoxin peroxidase 2). 2-D electrophoresis allowed us to identify new BALF proteins and to characterize protein composition in patients with sarcoidosis and idiophatic pulmonary fibrosis. Comparison of the gels of the two diseases showed that they differ in BALF protein profiles as they do in type of immune response. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/12373774/Bronchoalveolar_lavage_fluid_protein_composition_in_patients_with_sarcoidosis_and_idiopathic_pulmonary_fibrosis:_a_two_dimensional_electrophoretic_study_ L2 - https://doi.org/10.1002/1522-2683(200210)23:19<3434::AID-ELPS3434>3.0.CO;2-R DB - PRIME DP - Unbound Medicine ER -