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In vitro restoration of nitrate reductase: investigation of Aspergillus nidulans and Neurospora crassa nitrate reductase mutants.
Biochim Biophys Acta. 1975 Apr 07; 385(2):354-61.BB

Abstract

Extracts of Aspergillus nidulans wild type (bi-1) and the nitrate reductase mutant niaD-17 were active in the in vitro restoration of NADPH-dependent nitrate reductase when mixed with extracts of Neurospora crassa, nit-1. Among the A. nidulans cnx nitrate reductase mutants tested, only the molybdenum repair mutant, cnxE-14 grown in the presence of 10-minus 3 M Na2 MoO4 was active in the restoration assay. Aspergillus extracts contained an inhibitor(s) which was measured by the decrease in NADPH-dependent nitrate reductase formed when extracts of Rhodospirillum rubrum and N. crassa, nit-1 were incubated at room temperature. The inhibition by extracts of A. nidulans, bi-1, cnxE-14, cnxG-4 and cnxH-3 was a linear function of time and a logarithmic function of the protein concentration in the extract. The molybdenum content of N. crassa wild type and nit-1 mycelia were found to be similar, containing approx. 10 mu g molybdenum/mg dry mycelium. The NADPH-dependent cytochrome c reductase associated with nitrate reductase was purified from both strains. The NADPH-dependent cytochrome c reductase associated with nitrate reductase was purified from both strains. The enzyme purified from wild-type N. crassa contained more than 1 mol of molybdenum per mol of enzyme, whereas the enzyme purified from nit-1 contained negligible amounts of molybdenum.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

123779

Citation

Ketchum, P A., and R J. Downey. "In Vitro Restoration of Nitrate Reductase: Investigation of Aspergillus Nidulans and Neurospora Crassa Nitrate Reductase Mutants." Biochimica Et Biophysica Acta, vol. 385, no. 2, 1975, pp. 354-61.
Ketchum PA, Downey RJ. In vitro restoration of nitrate reductase: investigation of Aspergillus nidulans and Neurospora crassa nitrate reductase mutants. Biochim Biophys Acta. 1975;385(2):354-61.
Ketchum, P. A., & Downey, R. J. (1975). In vitro restoration of nitrate reductase: investigation of Aspergillus nidulans and Neurospora crassa nitrate reductase mutants. Biochimica Et Biophysica Acta, 385(2), 354-61.
Ketchum PA, Downey RJ. In Vitro Restoration of Nitrate Reductase: Investigation of Aspergillus Nidulans and Neurospora Crassa Nitrate Reductase Mutants. Biochim Biophys Acta. 1975 Apr 7;385(2):354-61. PubMed PMID: 123779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro restoration of nitrate reductase: investigation of Aspergillus nidulans and Neurospora crassa nitrate reductase mutants. AU - Ketchum,P A, AU - Downey,R J, PY - 1975/4/7/pubmed PY - 1975/4/7/medline PY - 1975/4/7/entrez SP - 354 EP - 61 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 385 IS - 2 N2 - Extracts of Aspergillus nidulans wild type (bi-1) and the nitrate reductase mutant niaD-17 were active in the in vitro restoration of NADPH-dependent nitrate reductase when mixed with extracts of Neurospora crassa, nit-1. Among the A. nidulans cnx nitrate reductase mutants tested, only the molybdenum repair mutant, cnxE-14 grown in the presence of 10-minus 3 M Na2 MoO4 was active in the restoration assay. Aspergillus extracts contained an inhibitor(s) which was measured by the decrease in NADPH-dependent nitrate reductase formed when extracts of Rhodospirillum rubrum and N. crassa, nit-1 were incubated at room temperature. The inhibition by extracts of A. nidulans, bi-1, cnxE-14, cnxG-4 and cnxH-3 was a linear function of time and a logarithmic function of the protein concentration in the extract. The molybdenum content of N. crassa wild type and nit-1 mycelia were found to be similar, containing approx. 10 mu g molybdenum/mg dry mycelium. The NADPH-dependent cytochrome c reductase associated with nitrate reductase was purified from both strains. The NADPH-dependent cytochrome c reductase associated with nitrate reductase was purified from both strains. The enzyme purified from wild-type N. crassa contained more than 1 mol of molybdenum per mol of enzyme, whereas the enzyme purified from nit-1 contained negligible amounts of molybdenum. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/123779/In_vitro_restoration_of_nitrate_reductase:_investigation_of_Aspergillus_nidulans_and_Neurospora_crassa_nitrate_reductase_mutants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0304-4165(75)90364-5 DB - PRIME DP - Unbound Medicine ER -