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The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
Cell Mol Biol Lett. 2002; 7(2B):671-6.CM

Abstract

Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.

Authors+Show Affiliations

Plant Breeding and Acclimatization Institute, Radzików, 05-870 Błonie, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12378226

Citation

Przetakiewicz, Jarosław, et al. "The Use of RAPD and Semi-random Markers to Verify Somatic Hybrids Between Diploid Lines of Solanum Tuberosum L." Cellular & Molecular Biology Letters, vol. 7, no. 2B, 2002, pp. 671-6.
Przetakiewicz J, Nadolska-Orczyk A, Orczyk W. The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L. Cell Mol Biol Lett. 2002;7(2B):671-6.
Przetakiewicz, J., Nadolska-Orczyk, A., & Orczyk, W. (2002). The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L. Cellular & Molecular Biology Letters, 7(2B), 671-6.
Przetakiewicz J, Nadolska-Orczyk A, Orczyk W. The Use of RAPD and Semi-random Markers to Verify Somatic Hybrids Between Diploid Lines of Solanum Tuberosum L. Cell Mol Biol Lett. 2002;7(2B):671-6. PubMed PMID: 12378226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L. AU - Przetakiewicz,Jarosław, AU - Nadolska-Orczyk,Anna, AU - Orczyk,Wacław, PY - 2002/10/16/pubmed PY - 2003/5/31/medline PY - 2002/10/16/entrez SP - 671 EP - 6 JF - Cellular & molecular biology letters JO - Cell Mol Biol Lett VL - 7 IS - 2B N2 - Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids. SN - 1425-8153 UR - https://www.unboundmedicine.com/medline/citation/12378226/The_use_of_RAPD_and_semi_random_markers_to_verify_somatic_hybrids_between_diploid_lines_of_Solanum_tuberosum_L_ DB - PRIME DP - Unbound Medicine ER -