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The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
Cell Mol Biol Lett. 2002; 7(2B):671-6.CM

Abstract

Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.

Authors+Show Affiliations

Przetakiewicz J
Plant Breeding and Acclimatization Institute, Radzików, 05-870 Błonie, Poland.
Nadolska-Orczyk A
No affiliation info available
Orczyk W
No affiliation info available

MeSH

Base SequenceDNA PrimersDiploidyGenetic MarkersHybridization, GeneticRandom Amplified Polymorphic DNA TechniqueSolanum tuberosum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12378226

Citation

Przetakiewicz, Jarosław, et al. "The Use of RAPD and Semi-random Markers to Verify Somatic Hybrids Between Diploid Lines of Solanum Tuberosum L." Cellular & Molecular Biology Letters, vol. 7, no. 2B, 2002, pp. 671-6.
Przetakiewicz J, Nadolska-Orczyk A, Orczyk W. The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L. Cell Mol Biol Lett. 2002;7(2B):671-6.
Przetakiewicz, J., Nadolska-Orczyk, A., & Orczyk, W. (2002). The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L. Cellular & Molecular Biology Letters, 7(2B), 671-6.
Przetakiewicz J, Nadolska-Orczyk A, Orczyk W. The Use of RAPD and Semi-random Markers to Verify Somatic Hybrids Between Diploid Lines of Solanum Tuberosum L. Cell Mol Biol Lett. 2002;7(2B):671-6. PubMed PMID: 12378226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L. AU - Przetakiewicz,Jarosław, AU - Nadolska-Orczyk,Anna, AU - Orczyk,Wacław, PY - 2002/10/16/pubmed PY - 2003/5/31/medline PY - 2002/10/16/entrez SP - 671 EP - 6 JF - Cellular & molecular biology letters JO - Cell Mol Biol Lett VL - 7 IS - 2B N2 - Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids. SN - 1425-8153 UR - https://www.unboundmedicine.com/medline/citation/12378226/The_use_of_RAPD_and_semi_random_markers_to_verify_somatic_hybrids_between_diploid_lines_of_Solanum_tuberosum_L_ DB - PRIME DP - Unbound Medicine ER -