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Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSR-PCR.
Cell Mol Biol Lett. 2002; 7(2B):785-94.CM

Abstract

The genotypes of the strawberry (Fragaria x ananassa), apple (Malus domestica) and Ribes species (R. nigrum, R. rubrum and R. glossularia), maintained in our Institute's collection and used in breeding programs, were screened for DNA markers. Twenty primers for RAPD (among 60 tested) and seven for ISSR (among 10 tested) were chosen as creating polymorphic DNA bands differentiating the investigated genotypes. Based on those identity markers, the genetic distance between genotypes was determined, and their relatedness was estimated. In many cases, both RAPD- and ISSR-based genetic similarity confirmed relatedness connected with biological origin and with the place where the cultivar was developed. However, some diversity connected with the technique used for molecular marker generation was observed. Generally, the similarity values based on ISSR data were higher than those based on RAPD. Parallel study using two data sets seems to enable a reduction in the number of potential mistakes connected with each method's, technical limitations and ensures more precise relatedness determination.

Authors+Show Affiliations

Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 Skierniewice, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12378239

Citation

Korbin, Małgorzata, et al. "Fruit Plant Germplasm Characterisation Using Molecular Markers Generated in RAPD and ISSR-PCR." Cellular & Molecular Biology Letters, vol. 7, no. 2B, 2002, pp. 785-94.
Korbin M, Kuras A, Zurawicz E. Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSR-PCR. Cell Mol Biol Lett. 2002;7(2B):785-94.
Korbin, M., Kuras, A., & Zurawicz, E. (2002). Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSR-PCR. Cellular & Molecular Biology Letters, 7(2B), 785-94.
Korbin M, Kuras A, Zurawicz E. Fruit Plant Germplasm Characterisation Using Molecular Markers Generated in RAPD and ISSR-PCR. Cell Mol Biol Lett. 2002;7(2B):785-94. PubMed PMID: 12378239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSR-PCR. AU - Korbin,Małgorzata, AU - Kuras,Anita, AU - Zurawicz,Edward, PY - 2002/10/16/pubmed PY - 2003/5/31/medline PY - 2002/10/16/entrez SP - 785 EP - 94 JF - Cellular & molecular biology letters JO - Cell Mol Biol Lett VL - 7 IS - 2B N2 - The genotypes of the strawberry (Fragaria x ananassa), apple (Malus domestica) and Ribes species (R. nigrum, R. rubrum and R. glossularia), maintained in our Institute's collection and used in breeding programs, were screened for DNA markers. Twenty primers for RAPD (among 60 tested) and seven for ISSR (among 10 tested) were chosen as creating polymorphic DNA bands differentiating the investigated genotypes. Based on those identity markers, the genetic distance between genotypes was determined, and their relatedness was estimated. In many cases, both RAPD- and ISSR-based genetic similarity confirmed relatedness connected with biological origin and with the place where the cultivar was developed. However, some diversity connected with the technique used for molecular marker generation was observed. Generally, the similarity values based on ISSR data were higher than those based on RAPD. Parallel study using two data sets seems to enable a reduction in the number of potential mistakes connected with each method's, technical limitations and ensures more precise relatedness determination. SN - 1425-8153 UR - https://www.unboundmedicine.com/medline/citation/12378239/Fruit_plant_germplasm_characterisation_using_molecular_markers_generated_in_RAPD_and_ISSR_PCR_ DB - PRIME DP - Unbound Medicine ER -