Tags

Type your tag names separated by a space and hit enter

Myogenic specification of side population cells in skeletal muscle.
J Cell Biol. 2002 Oct 14; 159(1):123-34.JC

Abstract

Skeletal muscle contains myogenic progenitors called satellite cells and muscle-derived stem cells that have been suggested to be pluripotent. We further investigated the differentiation potential of muscle-derived stem cells and satellite cells to elucidate relationships between these two populations of cells. FACS(R) analysis of muscle side population (SP) cells, a fraction of muscle-derived stem cells, revealed expression of hematopoietic stem cell marker Sca-1 but did not reveal expression of any satellite cell markers. Muscle SP cells were greatly enriched for cells competent to form hematopoietic colonies. Moreover, muscle SP cells with hematopoietic potential were CD45 positive. However, muscle SP cells did not differentiate into myocytes in vitro. By contrast, satellite cells gave rise to myocytes but did not express Sca-1 or CD45 and never formed hematopoietic colonies. Importantly, muscle SP cells exhibited the potential to give rise to both myocytes and satellite cells after intramuscular transplantation. In addition, muscle SP cells underwent myogenic specification after co-culture with myoblasts. Co-culture with myoblasts or forced expression of MyoD also induced muscle differentiation of muscle SP cells prepared from mice lacking Pax7 gene, an essential gene for satellite cell development. Therefore, these data document that satellite cells and muscle-derived stem cells represent distinct populations and demonstrate that muscle-derived stem cells have the potential to give rise to myogenic cells via a myocyte-mediated inductive interaction.

Authors+Show Affiliations

Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, K1H 8L6 Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12379804

Citation

Asakura, Atsushi, et al. "Myogenic Specification of Side Population Cells in Skeletal Muscle." The Journal of Cell Biology, vol. 159, no. 1, 2002, pp. 123-34.
Asakura A, Seale P, Girgis-Gabardo A, et al. Myogenic specification of side population cells in skeletal muscle. J Cell Biol. 2002;159(1):123-34.
Asakura, A., Seale, P., Girgis-Gabardo, A., & Rudnicki, M. A. (2002). Myogenic specification of side population cells in skeletal muscle. The Journal of Cell Biology, 159(1), 123-34.
Asakura A, et al. Myogenic Specification of Side Population Cells in Skeletal Muscle. J Cell Biol. 2002 Oct 14;159(1):123-34. PubMed PMID: 12379804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myogenic specification of side population cells in skeletal muscle. AU - Asakura,Atsushi, AU - Seale,Patrick, AU - Girgis-Gabardo,Adele, AU - Rudnicki,Michael A, Y1 - 2002/10/14/ PY - 2002/10/16/pubmed PY - 2002/11/26/medline PY - 2002/10/16/entrez SP - 123 EP - 34 JF - The Journal of cell biology JO - J. Cell Biol. VL - 159 IS - 1 N2 - Skeletal muscle contains myogenic progenitors called satellite cells and muscle-derived stem cells that have been suggested to be pluripotent. We further investigated the differentiation potential of muscle-derived stem cells and satellite cells to elucidate relationships between these two populations of cells. FACS(R) analysis of muscle side population (SP) cells, a fraction of muscle-derived stem cells, revealed expression of hematopoietic stem cell marker Sca-1 but did not reveal expression of any satellite cell markers. Muscle SP cells were greatly enriched for cells competent to form hematopoietic colonies. Moreover, muscle SP cells with hematopoietic potential were CD45 positive. However, muscle SP cells did not differentiate into myocytes in vitro. By contrast, satellite cells gave rise to myocytes but did not express Sca-1 or CD45 and never formed hematopoietic colonies. Importantly, muscle SP cells exhibited the potential to give rise to both myocytes and satellite cells after intramuscular transplantation. In addition, muscle SP cells underwent myogenic specification after co-culture with myoblasts. Co-culture with myoblasts or forced expression of MyoD also induced muscle differentiation of muscle SP cells prepared from mice lacking Pax7 gene, an essential gene for satellite cell development. Therefore, these data document that satellite cells and muscle-derived stem cells represent distinct populations and demonstrate that muscle-derived stem cells have the potential to give rise to myogenic cells via a myocyte-mediated inductive interaction. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/12379804/Myogenic_specification_of_side_population_cells_in_skeletal_muscle_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.200202092 DB - PRIME DP - Unbound Medicine ER -