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Neuroimmune interactions in guinea pig stomach and small intestine.
Am J Physiol Gastrointest Liver Physiol. 2003 Jan; 284(1):G154-64.AJ

Abstract

Enteric neuroimmune interactions in gastrointestinal hypersensitivity responses involve antigen detection by mast cells, mast cell degranulation, release of chemical mediators, and modulatory actions of the mediators on the enteric nervous system (ENS). Electrophysiological methods were used to investigate electrical and synaptic behavior of neurons in the stomach and small intestine during exposure to beta-lactoglobulin in guinea pigs sensitized to cow's milk. Application of beta-lactoglobulin to sensitized preparations depolarized the membrane potential and increased neuronal excitability in small intestinal neurons but not in gastric neurons. Effects on membrane potential and excitability in the small intestine were suppressed by the mast cell stabilizing drug ketotifen, the histamine H(2) receptor antagonist cimetidine, the cyclooxygenase inhibitor piroxicam, and the 5-lipoxygenase inhibitor caffeic acid. Unlike small intestinal ganglion cells, gastric myenteric neurons did not respond to histamine applied exogenously. Antigenic exposure suppressed noradrenergic inhibitory neurotransmission in the small intestinal submucosal plexus. The histamine H(3) receptor antagonist thioperamide and piroxicam, but not caffeic acid, prevented the allergic suppression of noradrenergic inhibitory neurotransmission. Antigenic stimulation of neuronal excitability and suppression of synaptic transmission occurred only in milk-sensitized animals. Results suggest that signaling between mast cells and the ENS underlies intestinal, but not gastric, anaphylactic responses associated with food allergies. Histamine, prostaglandins, and leukotrienes are paracrine signals in the communication pathway from mast cells to the small intestinal ENS.

Authors+Show Affiliations

Department of Physiology and Cell Biology, Ohio State University, College of Medicine and Public Health, Columbus 43210, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12388180

Citation

Liu, Sumei, et al. "Neuroimmune Interactions in Guinea Pig Stomach and Small Intestine." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 284, no. 1, 2003, pp. G154-64.
Liu S, Hu HZ, Gao N, et al. Neuroimmune interactions in guinea pig stomach and small intestine. Am J Physiol Gastrointest Liver Physiol. 2003;284(1):G154-64.
Liu, S., Hu, H. Z., Gao, N., Gao, C., Wang, G., Wang, X., Peck, O. C., Kim, G., Gao, X., Xia, Y., & Wood, J. D. (2003). Neuroimmune interactions in guinea pig stomach and small intestine. American Journal of Physiology. Gastrointestinal and Liver Physiology, 284(1), G154-64.
Liu S, et al. Neuroimmune Interactions in Guinea Pig Stomach and Small Intestine. Am J Physiol Gastrointest Liver Physiol. 2003;284(1):G154-64. PubMed PMID: 12388180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroimmune interactions in guinea pig stomach and small intestine. AU - Liu,Sumei, AU - Hu,Hong-Zhen, AU - Gao,Na, AU - Gao,Chuanyun, AU - Wang,Guodu, AU - Wang,Xiyu, AU - Peck,Owen C, AU - Kim,Gordon, AU - Gao,Xiang, AU - Xia,Yun, AU - Wood,Jackie D, Y1 - 2002/09/11/ PY - 2002/10/22/pubmed PY - 2003/1/24/medline PY - 2002/10/22/entrez SP - G154 EP - 64 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 284 IS - 1 N2 - Enteric neuroimmune interactions in gastrointestinal hypersensitivity responses involve antigen detection by mast cells, mast cell degranulation, release of chemical mediators, and modulatory actions of the mediators on the enteric nervous system (ENS). Electrophysiological methods were used to investigate electrical and synaptic behavior of neurons in the stomach and small intestine during exposure to beta-lactoglobulin in guinea pigs sensitized to cow's milk. Application of beta-lactoglobulin to sensitized preparations depolarized the membrane potential and increased neuronal excitability in small intestinal neurons but not in gastric neurons. Effects on membrane potential and excitability in the small intestine were suppressed by the mast cell stabilizing drug ketotifen, the histamine H(2) receptor antagonist cimetidine, the cyclooxygenase inhibitor piroxicam, and the 5-lipoxygenase inhibitor caffeic acid. Unlike small intestinal ganglion cells, gastric myenteric neurons did not respond to histamine applied exogenously. Antigenic exposure suppressed noradrenergic inhibitory neurotransmission in the small intestinal submucosal plexus. The histamine H(3) receptor antagonist thioperamide and piroxicam, but not caffeic acid, prevented the allergic suppression of noradrenergic inhibitory neurotransmission. Antigenic stimulation of neuronal excitability and suppression of synaptic transmission occurred only in milk-sensitized animals. Results suggest that signaling between mast cells and the ENS underlies intestinal, but not gastric, anaphylactic responses associated with food allergies. Histamine, prostaglandins, and leukotrienes are paracrine signals in the communication pathway from mast cells to the small intestinal ENS. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/12388180/Neuroimmune_interactions_in_guinea_pig_stomach_and_small_intestine_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00241.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -