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IGF alters jejunal glucose transporter expression and serum glucose levels in immature rats.

Abstract

Milk-borne insulin-like growth factors (IGFs) enhance nutrient absorption in the immature intestine, which is characterized by low levels of glucose oxidation. We therefore hypothesized that feeding a rat milk substitute (RMS) devoid of growth factors to rat pups would lower serum glucose levels relative to dam-fed control rats and that supplementation of RMS with physiological doses of either IGF-I or IGF-II would normalize serum glucose levels via increased jejunal glucose transporter 2 (GLUT2) and high-affinity Na(+)-glucose cotransporter (SGLT1) expression. We found lower serum glucose concentrations in RMS-fed pups; in contrast, serum glucose levels in the IGF-supplemented pups were similar to those of dam-fed controls. RT-PCR and laser scanning confocal microscopy similarly demonstrated that IGF supplementation increased expression of jejunal glucose transporters. Further experiments demonstrated that IGF supplementation altered mRNA levels of key mitochondrial enzymes without altering jejunal lactase activity. We conclude that IGF-I and IGF-II supplementation increases serum glucose levels in the immature rat pup fed artificial formula and alters gene expression of the jejunal glucose transporters.

Authors+Show Affiliations

Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, Mattel Children's Hospital, Los Angeles, California 90095-1752, USA. rlane@mednet.ucla.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12388463

Citation

Lane, Robert H., et al. "IGF Alters Jejunal Glucose Transporter Expression and Serum Glucose Levels in Immature Rats." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 283, no. 6, 2002, pp. R1450-60.
Lane RH, Dvorak B, MacLennan NK, et al. IGF alters jejunal glucose transporter expression and serum glucose levels in immature rats. Am J Physiol Regul Integr Comp Physiol. 2002;283(6):R1450-60.
Lane, R. H., Dvorak, B., MacLennan, N. K., Dvorakova, K., Halpern, M. D., Pham, T. D., & Philipps, A. F. (2002). IGF alters jejunal glucose transporter expression and serum glucose levels in immature rats. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 283(6), pp. R1450-60.
Lane RH, et al. IGF Alters Jejunal Glucose Transporter Expression and Serum Glucose Levels in Immature Rats. Am J Physiol Regul Integr Comp Physiol. 2002;283(6):R1450-60. PubMed PMID: 12388463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IGF alters jejunal glucose transporter expression and serum glucose levels in immature rats. AU - Lane,Robert H, AU - Dvorak,Bohuslav, AU - MacLennan,Nicole K, AU - Dvorakova,Katerina, AU - Halpern,Melissa D, AU - Pham,Tho D, AU - Philipps,Anthony F, Y1 - 2002/08/29/ PY - 2002/10/22/pubmed PY - 2002/12/19/medline PY - 2002/10/22/entrez SP - R1450 EP - 60 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am. J. Physiol. Regul. Integr. Comp. Physiol. VL - 283 IS - 6 N2 - Milk-borne insulin-like growth factors (IGFs) enhance nutrient absorption in the immature intestine, which is characterized by low levels of glucose oxidation. We therefore hypothesized that feeding a rat milk substitute (RMS) devoid of growth factors to rat pups would lower serum glucose levels relative to dam-fed control rats and that supplementation of RMS with physiological doses of either IGF-I or IGF-II would normalize serum glucose levels via increased jejunal glucose transporter 2 (GLUT2) and high-affinity Na(+)-glucose cotransporter (SGLT1) expression. We found lower serum glucose concentrations in RMS-fed pups; in contrast, serum glucose levels in the IGF-supplemented pups were similar to those of dam-fed controls. RT-PCR and laser scanning confocal microscopy similarly demonstrated that IGF supplementation increased expression of jejunal glucose transporters. Further experiments demonstrated that IGF supplementation altered mRNA levels of key mitochondrial enzymes without altering jejunal lactase activity. We conclude that IGF-I and IGF-II supplementation increases serum glucose levels in the immature rat pup fed artificial formula and alters gene expression of the jejunal glucose transporters. SN - 0363-6119 UR - https://www.unboundmedicine.com/medline/citation/12388463/IGF_alters_jejunal_glucose_transporter_expression_and_serum_glucose_levels_in_immature_rats_ L2 - http://www.physiology.org/doi/full/10.1152/ajpregu.00172.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -