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Activation of L-arginine transport in peripheral blood mononuclear cells in chronic renal failure.
Pflugers Arch. 2002 Oct; 445(1):147-51.PA

Abstract

Transport of LL-arginine, the precursor for nitric oxide (NO) synthesis, has been investigated in human peripheral blood mononuclear cells (PBMCs) obtained from healthy volunteers and chronic renal failure patients. Chronic renal failure patients were either on treatment by haemodialysis or continuous ambulatory peritoneal dialysis (CAPD). Saturable influx of L-arginine in PBMCs was mediated by the cationic amino acid transport systems y(+) and y(+)L. Initial rates of L-arginine transport (2 microM) via system y(+) were significantly increased in chronic renal failure patients, whereas transport via system y(+)L was unaffected. The increase in L-arginine transport via system y(+) was: 1.7-fold in uraemic patients on CAPD, 4.3-fold in uraemic patients pre-haemodialysis and 2.6-fold post-haemodialysis. When the intracellular PBMCs amino acid profile was analysed in chronic renal failure patients and control subjects, L-lysine and L-arginine concentrations were significantly increased in pre-haemodialysis uraemic patients and restored to normal values by haemodialysis and CAPD. The present study provides the first evidence that system y(+) mediates the increased transport of L-arginine in PBMCs from patients with chronic renal failure. The increased activity of system y(+) may provide the necessary supply of L-arginine to sustain NO synthesis in PBMCs exposed to increased levels of circulating cytokines in chronic renal failure.

Authors+Show Affiliations

Departmento de Farmacologia e Psicobiologia, Universidade do Estado do Rio de Janeiro, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12397399

Citation

Brunini, T M C., et al. "Activation of L-arginine Transport in Peripheral Blood Mononuclear Cells in Chronic Renal Failure." Pflugers Archiv : European Journal of Physiology, vol. 445, no. 1, 2002, pp. 147-51.
Brunini TM, Roberts NB, Yaqoob MM, et al. Activation of L-arginine transport in peripheral blood mononuclear cells in chronic renal failure. Pflugers Arch. 2002;445(1):147-51.
Brunini, T. M., Roberts, N. B., Yaqoob, M. M., Reis, P. F., Ellory, J. C., Mann, G. E., & Mendes-Ribeiro, A. C. (2002). Activation of L-arginine transport in peripheral blood mononuclear cells in chronic renal failure. Pflugers Archiv : European Journal of Physiology, 445(1), 147-51.
Brunini TM, et al. Activation of L-arginine Transport in Peripheral Blood Mononuclear Cells in Chronic Renal Failure. Pflugers Arch. 2002;445(1):147-51. PubMed PMID: 12397399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of L-arginine transport in peripheral blood mononuclear cells in chronic renal failure. AU - Brunini,T M C, AU - Roberts,N B, AU - Yaqoob,M M, AU - Reis,P F, AU - Ellory,J C, AU - Mann,G E, AU - Mendes-Ribeiro,A C, Y1 - 2002/08/29/ PY - 2002/04/26/received PY - 2002/07/09/accepted PY - 2002/10/25/pubmed PY - 2003/8/6/medline PY - 2002/10/25/entrez SP - 147 EP - 51 JF - Pflugers Archiv : European journal of physiology JO - Pflugers Arch VL - 445 IS - 1 N2 - Transport of LL-arginine, the precursor for nitric oxide (NO) synthesis, has been investigated in human peripheral blood mononuclear cells (PBMCs) obtained from healthy volunteers and chronic renal failure patients. Chronic renal failure patients were either on treatment by haemodialysis or continuous ambulatory peritoneal dialysis (CAPD). Saturable influx of L-arginine in PBMCs was mediated by the cationic amino acid transport systems y(+) and y(+)L. Initial rates of L-arginine transport (2 microM) via system y(+) were significantly increased in chronic renal failure patients, whereas transport via system y(+)L was unaffected. The increase in L-arginine transport via system y(+) was: 1.7-fold in uraemic patients on CAPD, 4.3-fold in uraemic patients pre-haemodialysis and 2.6-fold post-haemodialysis. When the intracellular PBMCs amino acid profile was analysed in chronic renal failure patients and control subjects, L-lysine and L-arginine concentrations were significantly increased in pre-haemodialysis uraemic patients and restored to normal values by haemodialysis and CAPD. The present study provides the first evidence that system y(+) mediates the increased transport of L-arginine in PBMCs from patients with chronic renal failure. The increased activity of system y(+) may provide the necessary supply of L-arginine to sustain NO synthesis in PBMCs exposed to increased levels of circulating cytokines in chronic renal failure. SN - 0031-6768 UR - https://www.unboundmedicine.com/medline/citation/12397399/Activation_of_L_arginine_transport_in_peripheral_blood_mononuclear_cells_in_chronic_renal_failure_ DB - PRIME DP - Unbound Medicine ER -