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Identification and distribution of the enterohemorrhagic Escherichia coli factor for adherence (efa1) gene in sorbitol-fermenting Escherichia coli O157: H-.
Int J Med Microbiol. 2002 Sep; 292(3-4):207-14.IJ

Abstract

Sorbitol-fermenting (SF) Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H- strains are emerging as causes of hemorrhagic colitis and the hemolytic-uremic syndrome in Europe. Using subtractive hybridization between SF STEC O157:H- strain 493/89 and STEC O157:H7 strain EDL933, three different fragments, of approximately 700 bp in length, were identified. Each demonstrated > 99% homology to genes encoding the enterohemorrhagic E. coli factor for adherence (efa1) and lymphostatin (lifA). Therefore, a cosmid library was constructed from SF STEC O157:H- strain 493/89, and one clone containing these fragments was sequenced. This sequencing demonstrated a 9669-bp open reading frame (ORF) that had 99.9% sequence homology to efa1 of STEC O111:H- strain E45035 and to lifA of an enteropathogenic E. coli O127:H6 strain E2348/69. In STEC O157:H7 strain EDL933, only small (ca. 3 kb) initial and terminal fragments of this ORF are present. PCR analysis with primers complementary to the efa1/lifA sequence of strain 493/89 indicated that the complete sequence is present in each of 10 SF STEC O157:H- isolates but in none of 10 STEC O157:H7 strains investigated. The presence of the complete efa1/lifA also in both tested E. coli O55:H7 strains supports the hypothesis that SF STEC O157:H- are phylogenetically closer to the proposed E. coli O55:H7 ancestor than STEC O157:H7. Our data demonstrate the presence of a potential virulence gene in SF STEC O157:H- that is only rudimentarily present in STEC O157:H7.

Authors+Show Affiliations

Institut für Hygiene, Universitätsklinikum Münster, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12398211

Citation

Janka, Andreas, et al. "Identification and Distribution of the Enterohemorrhagic Escherichia Coli Factor for Adherence (efa1) Gene in Sorbitol-fermenting Escherichia Coli O157: H-." International Journal of Medical Microbiology : IJMM, vol. 292, no. 3-4, 2002, pp. 207-14.
Janka A, Bielaszewska M, Dobrindt U, et al. Identification and distribution of the enterohemorrhagic Escherichia coli factor for adherence (efa1) gene in sorbitol-fermenting Escherichia coli O157: H-. Int J Med Microbiol. 2002;292(3-4):207-14.
Janka, A., Bielaszewska, M., Dobrindt, U., & Karch, H. (2002). Identification and distribution of the enterohemorrhagic Escherichia coli factor for adherence (efa1) gene in sorbitol-fermenting Escherichia coli O157: H-. International Journal of Medical Microbiology : IJMM, 292(3-4), 207-14.
Janka A, et al. Identification and Distribution of the Enterohemorrhagic Escherichia Coli Factor for Adherence (efa1) Gene in Sorbitol-fermenting Escherichia Coli O157: H-. Int J Med Microbiol. 2002;292(3-4):207-14. PubMed PMID: 12398211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and distribution of the enterohemorrhagic Escherichia coli factor for adherence (efa1) gene in sorbitol-fermenting Escherichia coli O157: H-. AU - Janka,Andreas, AU - Bielaszewska,Martina, AU - Dobrindt,Ulrich, AU - Karch,Helge, PY - 2002/10/26/pubmed PY - 2003/2/25/medline PY - 2002/10/26/entrez SP - 207 EP - 14 JF - International journal of medical microbiology : IJMM JO - Int J Med Microbiol VL - 292 IS - 3-4 N2 - Sorbitol-fermenting (SF) Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H- strains are emerging as causes of hemorrhagic colitis and the hemolytic-uremic syndrome in Europe. Using subtractive hybridization between SF STEC O157:H- strain 493/89 and STEC O157:H7 strain EDL933, three different fragments, of approximately 700 bp in length, were identified. Each demonstrated > 99% homology to genes encoding the enterohemorrhagic E. coli factor for adherence (efa1) and lymphostatin (lifA). Therefore, a cosmid library was constructed from SF STEC O157:H- strain 493/89, and one clone containing these fragments was sequenced. This sequencing demonstrated a 9669-bp open reading frame (ORF) that had 99.9% sequence homology to efa1 of STEC O111:H- strain E45035 and to lifA of an enteropathogenic E. coli O127:H6 strain E2348/69. In STEC O157:H7 strain EDL933, only small (ca. 3 kb) initial and terminal fragments of this ORF are present. PCR analysis with primers complementary to the efa1/lifA sequence of strain 493/89 indicated that the complete sequence is present in each of 10 SF STEC O157:H- isolates but in none of 10 STEC O157:H7 strains investigated. The presence of the complete efa1/lifA also in both tested E. coli O55:H7 strains supports the hypothesis that SF STEC O157:H- are phylogenetically closer to the proposed E. coli O55:H7 ancestor than STEC O157:H7. Our data demonstrate the presence of a potential virulence gene in SF STEC O157:H- that is only rudimentarily present in STEC O157:H7. SN - 1438-4221 UR - https://www.unboundmedicine.com/medline/citation/12398211/Identification_and_distribution_of_the_enterohemorrhagic_Escherichia_coli_factor_for_adherence__efa1__gene_in_sorbitol_fermenting_Escherichia_coli_O157:_H__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1438-4221(04)70099-5 DB - PRIME DP - Unbound Medicine ER -