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A high phylloquinone intake is required to achieve maximal osteocalcin gamma-carboxylation.

Abstract

BACKGROUND

Dietary vitamin K is usually inadequate to maximize serum osteocalcin gamma-carboxylation. Phylloquinone supplementation increases osteocalcin gamma-carboxylation; however, the amount required to maximize carboxylation is not known.

OBJECTIVE

This study assessed the ability of various doses of phylloquinone (vitamin K(1)) to facilitate osteocalcin gamma-carboxylation.

DESIGN

Healthy adults aged 19-36 y participated in 2 substudies. In an initial dose-finding study (substudy A), 6 women and 4 men received a placebo daily for 1 wk and then phylloquinone daily for 3 wk: 500, 1000, and 2000 micro g during weeks 2, 3, and 4, respectively. Osteocalcin and undercarboxylated osteocalcin were measured at baseline and after each week of supplementation. Subsequently, to further delineate the gamma-carboxylation response of osteocalcin to various doses of vitamin K, 58 women and 42 men were randomly assigned to receive placebo or phylloquinone supplementation (250, 375, 500, and 1000 micro g/d) for 2 wk (substudy B). The percentage of undercarboxylated osteocalcin (%ucOC) was measured at baseline and weeks 1 and 2.

RESULTS

In substudy A, %ucOC decreased with phylloquinone supplementation (P < 0.0001); a greater reduction was observed with 1000 and 2000 micro g than with 500 micro g (P < 0.05). In substudy B, %ucOC decreased in all supplemented groups by week 1 (P for the trend < 0.0001), which was sustained through week 2. Phylloquinone supplementation decreased %ucOC dose-dependently; %ucOC was significantly different between the 250- micro g and the placebo groups and between the 1000- and 500- micro g groups but not between the 250-, 375-, and 500- micro g groups.

CONCLUSION

A daily phylloquinone intake of approximately 1000 micro g is required to maximally gamma-carboxylate circulating osteocalcin.

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  • Authors+Show Affiliations

    ,

    Institute on Aging, Department of Medicine, University of Wisconsin, Madison, USA. nbinkley@facstaff.wisc.edu

    , , , ,

    Source

    MeSH

    Adult
    Dietary Supplements
    Dose-Response Relationship, Drug
    Female
    Humans
    Male
    Osteocalcin
    Vitamin K 1

    Pub Type(s)

    Clinical Trial
    Journal Article
    Randomized Controlled Trial
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    12399278

    Citation

    Binkley, Neil C., et al. "A High Phylloquinone Intake Is Required to Achieve Maximal Osteocalcin Gamma-carboxylation." The American Journal of Clinical Nutrition, vol. 76, no. 5, 2002, pp. 1055-60.
    Binkley NC, Krueger DC, Kawahara TN, et al. A high phylloquinone intake is required to achieve maximal osteocalcin gamma-carboxylation. Am J Clin Nutr. 2002;76(5):1055-60.
    Binkley, N. C., Krueger, D. C., Kawahara, T. N., Engelke, J. A., Chappell, R. J., & Suttie, J. W. (2002). A high phylloquinone intake is required to achieve maximal osteocalcin gamma-carboxylation. The American Journal of Clinical Nutrition, 76(5), pp. 1055-60.
    Binkley NC, et al. A High Phylloquinone Intake Is Required to Achieve Maximal Osteocalcin Gamma-carboxylation. Am J Clin Nutr. 2002;76(5):1055-60. PubMed PMID: 12399278.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - A high phylloquinone intake is required to achieve maximal osteocalcin gamma-carboxylation. AU - Binkley,Neil C, AU - Krueger,Diane C, AU - Kawahara,Tisha N, AU - Engelke,Jean A, AU - Chappell,Richard J, AU - Suttie,John W, PY - 2002/10/26/pubmed PY - 2002/11/28/medline PY - 2002/10/26/entrez SP - 1055 EP - 60 JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. VL - 76 IS - 5 N2 - BACKGROUND: Dietary vitamin K is usually inadequate to maximize serum osteocalcin gamma-carboxylation. Phylloquinone supplementation increases osteocalcin gamma-carboxylation; however, the amount required to maximize carboxylation is not known. OBJECTIVE: This study assessed the ability of various doses of phylloquinone (vitamin K(1)) to facilitate osteocalcin gamma-carboxylation. DESIGN: Healthy adults aged 19-36 y participated in 2 substudies. In an initial dose-finding study (substudy A), 6 women and 4 men received a placebo daily for 1 wk and then phylloquinone daily for 3 wk: 500, 1000, and 2000 micro g during weeks 2, 3, and 4, respectively. Osteocalcin and undercarboxylated osteocalcin were measured at baseline and after each week of supplementation. Subsequently, to further delineate the gamma-carboxylation response of osteocalcin to various doses of vitamin K, 58 women and 42 men were randomly assigned to receive placebo or phylloquinone supplementation (250, 375, 500, and 1000 micro g/d) for 2 wk (substudy B). The percentage of undercarboxylated osteocalcin (%ucOC) was measured at baseline and weeks 1 and 2. RESULTS: In substudy A, %ucOC decreased with phylloquinone supplementation (P < 0.0001); a greater reduction was observed with 1000 and 2000 micro g than with 500 micro g (P < 0.05). In substudy B, %ucOC decreased in all supplemented groups by week 1 (P for the trend < 0.0001), which was sustained through week 2. Phylloquinone supplementation decreased %ucOC dose-dependently; %ucOC was significantly different between the 250- micro g and the placebo groups and between the 1000- and 500- micro g groups but not between the 250-, 375-, and 500- micro g groups. CONCLUSION: A daily phylloquinone intake of approximately 1000 micro g is required to maximally gamma-carboxylate circulating osteocalcin. SN - 0002-9165 UR - https://www.unboundmedicine.com/medline/citation/12399278/A_high_phylloquinone_intake_is_required_to_achieve_maximal_osteocalcin_gamma_carboxylation_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.1093/ajcn/76.5.1055 DB - PRIME DP - Unbound Medicine ER -