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[Catalytic properties of cytoplasmic and mitochondrial aconitate hydratase from rat cardiomyocytes].
Izv Akad Nauk Ser Biol. 2002 Sep-OctIA

Abstract

Enzymatic activity of aconitate hydratase (aconitase, EC 4.2.1.3) from the rat heart is localized in the cytoplasm (65%) and mitochondria (35%). Cytoplasmic and mitochondrial forms of aconitate hydratase were separated by ion-exchange chromatography on CM-Cellulose and CM-Sephadex. The two forms have similar molecular weight, optimal pH range, and spectral properties; however, they have different chromatography properties, Km for citrate and isocitrate, as well as sensitivity to Fe2+ ions.

Authors+Show Affiliations

Medvedeva LV
Voronezh State University, Universitetskaya pl. 1, Voronezh, 394693 Russia.
Popova TN
No affiliation info available
Artiukhov VG
No affiliation info available
Matasova LV
No affiliation info available

MeSH

Aconitate HydrataseAnimalsCatalysisChromatography, Ion ExchangeCitric AcidCytoplasmHydrogen-Ion ConcentrationIronIsocitratesMaleMitochondria, HeartMolecular WeightMyocytes, CardiacRats

Pub Type(s)

Journal Article

Language

rus

PubMed ID

12400375

Citation

Medvedeva, L V., et al. "[Catalytic Properties of Cytoplasmic and Mitochondrial Aconitate Hydratase From Rat Cardiomyocytes]." Izvestiia Akademii Nauk. Seriia Biologicheskaia, 2002, pp. 528-33.
Medvedeva LV, Popova TN, Artiukhov VG, et al. [Catalytic properties of cytoplasmic and mitochondrial aconitate hydratase from rat cardiomyocytes]. Izv Akad Nauk Ser Biol. 2002.
Medvedeva, L. V., Popova, T. N., Artiukhov, V. G., & Matasova, L. V. (2002). [Catalytic properties of cytoplasmic and mitochondrial aconitate hydratase from rat cardiomyocytes]. Izvestiia Akademii Nauk. Seriia Biologicheskaia, (5), 528-33.
Medvedeva LV, et al. [Catalytic Properties of Cytoplasmic and Mitochondrial Aconitate Hydratase From Rat Cardiomyocytes]. Izv Akad Nauk Ser Biol. 2002 Sep-Oct;(5)528-33. PubMed PMID: 12400375.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Catalytic properties of cytoplasmic and mitochondrial aconitate hydratase from rat cardiomyocytes]. AU - Medvedeva,L V, AU - Popova,T N, AU - Artiukhov,V G, AU - Matasova,L V, PY - 2002/10/29/pubmed PY - 2003/1/17/medline PY - 2002/10/29/entrez SP - 528 EP - 33 JF - Izvestiia Akademii nauk. Seriia biologicheskaia JO - Izv Akad Nauk Ser Biol IS - 5 N2 - Enzymatic activity of aconitate hydratase (aconitase, EC 4.2.1.3) from the rat heart is localized in the cytoplasm (65%) and mitochondria (35%). Cytoplasmic and mitochondrial forms of aconitate hydratase were separated by ion-exchange chromatography on CM-Cellulose and CM-Sephadex. The two forms have similar molecular weight, optimal pH range, and spectral properties; however, they have different chromatography properties, Km for citrate and isocitrate, as well as sensitivity to Fe2+ ions. SN - 1026-3470 UR - https://www.unboundmedicine.com/medline/citation/12400375/[Catalytic_properties_of_cytoplasmic_and_mitochondrial_aconitate_hydratase_from_rat_cardiomyocytes]_ DB - PRIME DP - Unbound Medicine ER -