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Myosin heavy chain composition of single muscle fibers in male distance runners.
Int J Sports Med. 2002 Oct; 23(7):484-8.IJ

Abstract

The purpose of this study was to characterize the myosin heavy chain (MHC) composition of single muscle fibers from the gastrocnemius of male collegiate distance (DIST; n = 7), middle-distance (MID; n = 6), and recreational runners (REC; n = 6). Additionally, mATPase histochemistry was used to serve as a comparison to previous studies and the single fiber MHC technique. SDS-PAGE of single muscle fibers revealed a higher proportion of MHC I in DIST compared to MID and REC (74.9 +/- 4.3 vs 54.4 +/- 2.8 vs 56.2 +/- 2.9 %, respectively; p < 0.05), less MHC IIa/IIx in DIST compared to MID and REC (0.0 +/- 0.0 vs 6.0 +/- 2.4 vs 15.9 +/- 4.2 %, respectively; p < 0.05), and more total hybrids (I/IIa+IIa/IIx+I/IIa/IIx) in REC than both run groups, DIST and MID (23.0 +/- 3.3 vs 6.2 +/- 1.1 vs 13.2 +/- 2.6 %, respectively; p < 0.05). ATPase histochemistry (pH 4.54) revealed a higher percentage of type I fibers in DIST compared to MID and REC (71.1 +/- 3.1 vs 56.3 +/- 2.5 vs 59.8 +/- 2.3 %, respectively; p < 0.05), a higher percentage of type IIa in MID compared to DIST and REC (43.3 +/- 2.7 vs 28.5 +/- 3.1 vs. 30.2 +/- 3.1 %, p < 0.05), and a higher distribution of type IIb in REC than both run groups (10.0 +/- 2.7 vs 0.4 +/- 0.2 vs 0.4 +/- 0.2 %, p < 0.05). These results suggest that distance running leads to an increase in MHC I expression, training for mid-distance events leads to a prevalence of MHC IIa, and run training leads to a decrease in hybrid fibers.

Authors+Show Affiliations

Human Performance Laboratory, Ball State University, Muncie, Indiana 47306, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12402179

Citation

Harber, M P., et al. "Myosin Heavy Chain Composition of Single Muscle Fibers in Male Distance Runners." International Journal of Sports Medicine, vol. 23, no. 7, 2002, pp. 484-8.
Harber MP, Gallagher PM, Trautmann J, et al. Myosin heavy chain composition of single muscle fibers in male distance runners. Int J Sports Med. 2002;23(7):484-8.
Harber, M. P., Gallagher, P. M., Trautmann, J., & Trappe, S. W. (2002). Myosin heavy chain composition of single muscle fibers in male distance runners. International Journal of Sports Medicine, 23(7), 484-8.
Harber MP, et al. Myosin Heavy Chain Composition of Single Muscle Fibers in Male Distance Runners. Int J Sports Med. 2002;23(7):484-8. PubMed PMID: 12402179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myosin heavy chain composition of single muscle fibers in male distance runners. AU - Harber,M P, AU - Gallagher,P M, AU - Trautmann,J, AU - Trappe,S W, PY - 2002/10/29/pubmed PY - 2003/3/14/medline PY - 2002/10/29/entrez SP - 484 EP - 8 JF - International journal of sports medicine JO - Int J Sports Med VL - 23 IS - 7 N2 - The purpose of this study was to characterize the myosin heavy chain (MHC) composition of single muscle fibers from the gastrocnemius of male collegiate distance (DIST; n = 7), middle-distance (MID; n = 6), and recreational runners (REC; n = 6). Additionally, mATPase histochemistry was used to serve as a comparison to previous studies and the single fiber MHC technique. SDS-PAGE of single muscle fibers revealed a higher proportion of MHC I in DIST compared to MID and REC (74.9 +/- 4.3 vs 54.4 +/- 2.8 vs 56.2 +/- 2.9 %, respectively; p < 0.05), less MHC IIa/IIx in DIST compared to MID and REC (0.0 +/- 0.0 vs 6.0 +/- 2.4 vs 15.9 +/- 4.2 %, respectively; p < 0.05), and more total hybrids (I/IIa+IIa/IIx+I/IIa/IIx) in REC than both run groups, DIST and MID (23.0 +/- 3.3 vs 6.2 +/- 1.1 vs 13.2 +/- 2.6 %, respectively; p < 0.05). ATPase histochemistry (pH 4.54) revealed a higher percentage of type I fibers in DIST compared to MID and REC (71.1 +/- 3.1 vs 56.3 +/- 2.5 vs 59.8 +/- 2.3 %, respectively; p < 0.05), a higher percentage of type IIa in MID compared to DIST and REC (43.3 +/- 2.7 vs 28.5 +/- 3.1 vs. 30.2 +/- 3.1 %, p < 0.05), and a higher distribution of type IIb in REC than both run groups (10.0 +/- 2.7 vs 0.4 +/- 0.2 vs 0.4 +/- 0.2 %, p < 0.05). These results suggest that distance running leads to an increase in MHC I expression, training for mid-distance events leads to a prevalence of MHC IIa, and run training leads to a decrease in hybrid fibers. SN - 0172-4622 UR - https://www.unboundmedicine.com/medline/citation/12402179/Myosin_heavy_chain_composition_of_single_muscle_fibers_in_male_distance_runners_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2002-35067 DB - PRIME DP - Unbound Medicine ER -