Tags

Type your tag names separated by a space and hit enter

Chromosomal location of 46 new RAPD markers in rye (Secale cereale L.).
Genetica. 2002 Jun; 115(2):205-11.G

Abstract

The polymerase chain reaction (PCR) was used to locate RAPD markers using disomic wheat-rye addition lines in order to develop a set of molecular markers distributed on the seven rye chromosomes. We carried out RAPD amplifications on genomic DNA of wheat 'Chinese Spring' (CS), rye 'Imperial' (I), the amphiploid wheat-rye and the seven disomic wheat-rye addition lines (1R-7R) using 140 different 10-mer oligonucleotides. Forty six new RAPD markers were located on the seven rye chromosomes and all the disomic wheat-rye addition lines were identified on the basis of their amplification patterns. The number of RAPD bands located on 1R, 2R, 3R, 4R, 5R, 6R and 7R chromosomes were 5, 8, 11, 8, 8, 10 and 6, respectively. The seven wheat-rye addition lines can be distinguished using only the following three 10-mer oligonucleotides: OPA16, OPF19 and GEN3-605, the other RAPD primers being useful for this purpose. The use of these RAPDs as a source of molecular markers that could be linked to interesting genes or other important agronomic traits is discussed.

Authors+Show Affiliations

González C
Laboratory of Genetics, Universidad San Pablo, CEU, Madrid, Spain.
Camacho MV
No affiliation info available
Benito C
No affiliation info available

MeSH

Chromosome MappingChromosomes, PlantGenetic MarkersGenome, PlantPolymerase Chain ReactionRandom Amplified Polymorphic DNA TechniqueSecaleSequence Analysis, DNATriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12403175

Citation

González, Cecilia, et al. "Chromosomal Location of 46 New RAPD Markers in Rye (Secale Cereale L.)." Genetica, vol. 115, no. 2, 2002, pp. 205-11.
González C, Camacho MV, Benito C. Chromosomal location of 46 new RAPD markers in rye (Secale cereale L.). Genetica. 2002;115(2):205-11.
González, C., Camacho, M. V., & Benito, C. (2002). Chromosomal location of 46 new RAPD markers in rye (Secale cereale L.). Genetica, 115(2), 205-11.
González C, Camacho MV, Benito C. Chromosomal Location of 46 New RAPD Markers in Rye (Secale Cereale L.). Genetica. 2002;115(2):205-11. PubMed PMID: 12403175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosomal location of 46 new RAPD markers in rye (Secale cereale L.). AU - González,Cecilia, AU - Camacho,Manuel V, AU - Benito,César, PY - 2002/10/31/pubmed PY - 2003/4/5/medline PY - 2002/10/31/entrez SP - 205 EP - 11 JF - Genetica JO - Genetica VL - 115 IS - 2 N2 - The polymerase chain reaction (PCR) was used to locate RAPD markers using disomic wheat-rye addition lines in order to develop a set of molecular markers distributed on the seven rye chromosomes. We carried out RAPD amplifications on genomic DNA of wheat 'Chinese Spring' (CS), rye 'Imperial' (I), the amphiploid wheat-rye and the seven disomic wheat-rye addition lines (1R-7R) using 140 different 10-mer oligonucleotides. Forty six new RAPD markers were located on the seven rye chromosomes and all the disomic wheat-rye addition lines were identified on the basis of their amplification patterns. The number of RAPD bands located on 1R, 2R, 3R, 4R, 5R, 6R and 7R chromosomes were 5, 8, 11, 8, 8, 10 and 6, respectively. The seven wheat-rye addition lines can be distinguished using only the following three 10-mer oligonucleotides: OPA16, OPF19 and GEN3-605, the other RAPD primers being useful for this purpose. The use of these RAPDs as a source of molecular markers that could be linked to interesting genes or other important agronomic traits is discussed. SN - 0016-6707 UR - https://www.unboundmedicine.com/medline/citation/12403175/Chromosomal_location_of_46_new_RAPD_markers_in_rye__Secale_cereale_L___ DB - PRIME DP - Unbound Medicine ER -