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An orchid (Oncidium Gower Ramsey) AP3-like MADS gene regulates floral formation and initiation.
Plant Cell Physiol. 2002 Oct; 43(10):1198-209.PC

Abstract

cDNA for a B group MADS box gene OMADS3 was isolated and characterized from Oncidium Gower Ramsey, an important species of orchid. OMADS3 encoding a 204 amino acid protein showed high sequence homology to both paleoAP3 and TM6 lineage of B group MADS box gene such as monocots AP3 homologue LMADS1 in lily and GDEF1 in Gerbera hybrida. Despite the sequence homology, consensus motifs identified in the C-terminal region of B group genes were absent in OMADS3. Southern analysis indicated that OMADS3 was present in O. Gower Ramsey genome in low copy numbers. Different from most B group genes, OMADS3 mRNA was detected in all four floral organs as well as in vegetative leaves. This is similar to the expression pattern of GDEF1. 35S::OMADS3 transgenic plants showed novel phenotypes by producing terminal flowers similar to those observed in transgenic plants ectopically expressed A functional genes such as AP1. Ectopic expression of OMADS3 cDNA truncated with the MADS box or C terminal region in Arabidopsis generated novel ap2-like flowers in which sepals and petals were converted into carpel-like and stamen-like structures. Yeast two-hybrid analysis indicated that OMADS3 is able to strongly form homodimers. Our results suggested that OMADS3 might represent an ancestral form of TM6-like gene which was conserved in monocots with a function similar to A functional gene in regulating flower formation as well as floral initiation.

Authors+Show Affiliations

Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung, Taiwan, 40227 ROC.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12407200

Citation

Hsu, Hsing-Fun, and Chang-Hsien Yang. "An Orchid (Oncidium Gower Ramsey) AP3-like MADS Gene Regulates Floral Formation and Initiation." Plant & Cell Physiology, vol. 43, no. 10, 2002, pp. 1198-209.
Hsu HF, Yang CH. An orchid (Oncidium Gower Ramsey) AP3-like MADS gene regulates floral formation and initiation. Plant Cell Physiol. 2002;43(10):1198-209.
Hsu, H. F., & Yang, C. H. (2002). An orchid (Oncidium Gower Ramsey) AP3-like MADS gene regulates floral formation and initiation. Plant & Cell Physiology, 43(10), 1198-209.
Hsu HF, Yang CH. An Orchid (Oncidium Gower Ramsey) AP3-like MADS Gene Regulates Floral Formation and Initiation. Plant Cell Physiol. 2002;43(10):1198-209. PubMed PMID: 12407200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An orchid (Oncidium Gower Ramsey) AP3-like MADS gene regulates floral formation and initiation. AU - Hsu,Hsing-Fun, AU - Yang,Chang-Hsien, PY - 2002/10/31/pubmed PY - 2003/3/5/medline PY - 2002/10/31/entrez SP - 1198 EP - 209 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 43 IS - 10 N2 - cDNA for a B group MADS box gene OMADS3 was isolated and characterized from Oncidium Gower Ramsey, an important species of orchid. OMADS3 encoding a 204 amino acid protein showed high sequence homology to both paleoAP3 and TM6 lineage of B group MADS box gene such as monocots AP3 homologue LMADS1 in lily and GDEF1 in Gerbera hybrida. Despite the sequence homology, consensus motifs identified in the C-terminal region of B group genes were absent in OMADS3. Southern analysis indicated that OMADS3 was present in O. Gower Ramsey genome in low copy numbers. Different from most B group genes, OMADS3 mRNA was detected in all four floral organs as well as in vegetative leaves. This is similar to the expression pattern of GDEF1. 35S::OMADS3 transgenic plants showed novel phenotypes by producing terminal flowers similar to those observed in transgenic plants ectopically expressed A functional genes such as AP1. Ectopic expression of OMADS3 cDNA truncated with the MADS box or C terminal region in Arabidopsis generated novel ap2-like flowers in which sepals and petals were converted into carpel-like and stamen-like structures. Yeast two-hybrid analysis indicated that OMADS3 is able to strongly form homodimers. Our results suggested that OMADS3 might represent an ancestral form of TM6-like gene which was conserved in monocots with a function similar to A functional gene in regulating flower formation as well as floral initiation. SN - 0032-0781 UR - https://www.unboundmedicine.com/medline/citation/12407200/An_orchid__Oncidium_Gower_Ramsey__AP3_like_MADS_gene_regulates_floral_formation_and_initiation_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcf143 DB - PRIME DP - Unbound Medicine ER -