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Defining characteristics of Types I and II apoptotic cells in response to TRAIL.
Neoplasia. 2002 Nov-Dec; 4(6):551-7.N

Abstract

Type I cells have been defined to be independent of mitochondria for the induction of Fas death receptor-mediated apoptosis, whereas Type II cells are mitochondria-dependent. Knock-out studies in mice show that thymocytes are Type I and liver cells are Type II. We have previously shown that primary human hepatocytes and HCT116 human colon carcinoma cells behave like Type II cells because TRAIL-induced apoptosis can be blocked by the caspase 9 inhibitor, Z-LEHD-FMK. On the other hand, caspase 9 inhibition does not allow survival of TRAIL-treated SW480 colon cancer cells, which is predicted for Type I cells. Investigating the differences in TRAIL-induced apoptotic pathways in HCT116 and SW480 cells revealed that although FADD, BID, and procaspase 3 protein levels are higher in SW480 cells, and although procaspase 8 and FLIP processing is more efficient at the TRAIL-DISC of SW480 cells, BID, procaspase 3, XIAP, and PARP cleavages occur more rapidly in HCT116, despite the higher levels of BCL-2 and HSP70. Cytochrome c release from the mitochondria to the cytoplasm is more efficient in HCT116 cells. These results suggest BID cleavage as a possible limiting factor in the involvement of mitochondria in TRAIL-induced cell death. Thus, regulation of BID cleavage may define if a cell is mitochondria-dependent or -independent in response to TRAIL death receptor-induced apoptosis.

Authors+Show Affiliations

Laboratory of Molecular Oncology and Cell Cycle Regulation, Howard Hughes Medical Institute, Philadelphia, PA 19104, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12407450

Citation

Ozören, Nesrin, and Wafik S. El-Deiry. "Defining Characteristics of Types I and II Apoptotic Cells in Response to TRAIL." Neoplasia (New York, N.Y.), vol. 4, no. 6, 2002, pp. 551-7.
Ozören N, El-Deiry WS. Defining characteristics of Types I and II apoptotic cells in response to TRAIL. Neoplasia. 2002;4(6):551-7.
Ozören, N., & El-Deiry, W. S. (2002). Defining characteristics of Types I and II apoptotic cells in response to TRAIL. Neoplasia (New York, N.Y.), 4(6), 551-7.
Ozören N, El-Deiry WS. Defining Characteristics of Types I and II Apoptotic Cells in Response to TRAIL. Neoplasia. 2002 Nov-Dec;4(6):551-7. PubMed PMID: 12407450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Defining characteristics of Types I and II apoptotic cells in response to TRAIL. AU - Ozören,Nesrin, AU - El-Deiry,Wafik S, PY - 2002/04/15/received PY - 2002/06/14/accepted PY - 2002/10/31/pubmed PY - 2003/4/30/medline PY - 2002/10/31/entrez SP - 551 EP - 7 JF - Neoplasia (New York, N.Y.) JO - Neoplasia VL - 4 IS - 6 N2 - Type I cells have been defined to be independent of mitochondria for the induction of Fas death receptor-mediated apoptosis, whereas Type II cells are mitochondria-dependent. Knock-out studies in mice show that thymocytes are Type I and liver cells are Type II. We have previously shown that primary human hepatocytes and HCT116 human colon carcinoma cells behave like Type II cells because TRAIL-induced apoptosis can be blocked by the caspase 9 inhibitor, Z-LEHD-FMK. On the other hand, caspase 9 inhibition does not allow survival of TRAIL-treated SW480 colon cancer cells, which is predicted for Type I cells. Investigating the differences in TRAIL-induced apoptotic pathways in HCT116 and SW480 cells revealed that although FADD, BID, and procaspase 3 protein levels are higher in SW480 cells, and although procaspase 8 and FLIP processing is more efficient at the TRAIL-DISC of SW480 cells, BID, procaspase 3, XIAP, and PARP cleavages occur more rapidly in HCT116, despite the higher levels of BCL-2 and HSP70. Cytochrome c release from the mitochondria to the cytoplasm is more efficient in HCT116 cells. These results suggest BID cleavage as a possible limiting factor in the involvement of mitochondria in TRAIL-induced cell death. Thus, regulation of BID cleavage may define if a cell is mitochondria-dependent or -independent in response to TRAIL death receptor-induced apoptosis. SN - 1522-8002 UR - https://www.unboundmedicine.com/medline/citation/12407450/Defining_characteristics_of_Types_I_and_II_apoptotic_cells_in_response_to_TRAIL_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/12407450/ DB - PRIME DP - Unbound Medicine ER -