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Overexpression of cyclin A in hepatocellular carcinoma and its relationship with HBx gene integration.
Zhonghua Zhong Liu Za Zhi. 2002 Jul; 24(4):353-5.ZZ

Abstract

OBJECTIVE

To investigate the frequency of cyclin A overexpression in hepatocellular carcinoma (HCC) and its relationship with clinical significance and HBx gene integration.

METHODS

PCR, RT-PCR and Western blot were used to detect the gene, mRNA and protein level of cyclin A in the tumor and nontumorous tissue. PCR and Southern blot were used to detect the integration of HBx gene in HCC.

RESULTS

Amplification of cyclin A gene was found in 1 of 35 patients; overexpression of cyclin A mRNA and protein was found in 16 of 35, 21 of 35 patients, respectively. Overexpression of cyclin A protein was correlated with patient's age, tumor size and HBx integration.

CONCLUSION

Overexpression of cyclin A occurs in the early stage of HCC carcinogenesis. It may be one of the important approaches by which HBV affects the normal cell cycle of hepatocyte.

Authors+Show Affiliations

Department of General Surgery, First People's Hospital, Shanghai 200080, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12408762

Citation

Zhang, Yi, et al. "Overexpression of Cyclin a in Hepatocellular Carcinoma and Its Relationship With HBx Gene Integration." Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], vol. 24, no. 4, 2002, pp. 353-5.
Zhang Y, Peng Z, Qiu G, et al. Overexpression of cyclin A in hepatocellular carcinoma and its relationship with HBx gene integration. Zhonghua Zhong Liu Za Zhi. 2002;24(4):353-5.
Zhang, Y., Peng, Z., Qiu, G., Wang, Z., & Gu, W. (2002). Overexpression of cyclin A in hepatocellular carcinoma and its relationship with HBx gene integration. Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], 24(4), 353-5.
Zhang Y, et al. Overexpression of Cyclin a in Hepatocellular Carcinoma and Its Relationship With HBx Gene Integration. Zhonghua Zhong Liu Za Zhi. 2002;24(4):353-5. PubMed PMID: 12408762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of cyclin A in hepatocellular carcinoma and its relationship with HBx gene integration. AU - Zhang,Yi, AU - Peng,Zhihai, AU - Qiu,Guoqiang, AU - Wang,Zhaowen, AU - Gu,Wei, PY - 2002/11/1/pubmed PY - 2003/1/8/medline PY - 2002/11/1/entrez SP - 353 EP - 5 JF - Zhonghua zhong liu za zhi [Chinese journal of oncology] JO - Zhonghua Zhong Liu Za Zhi VL - 24 IS - 4 N2 - OBJECTIVE: To investigate the frequency of cyclin A overexpression in hepatocellular carcinoma (HCC) and its relationship with clinical significance and HBx gene integration. METHODS: PCR, RT-PCR and Western blot were used to detect the gene, mRNA and protein level of cyclin A in the tumor and nontumorous tissue. PCR and Southern blot were used to detect the integration of HBx gene in HCC. RESULTS: Amplification of cyclin A gene was found in 1 of 35 patients; overexpression of cyclin A mRNA and protein was found in 16 of 35, 21 of 35 patients, respectively. Overexpression of cyclin A protein was correlated with patient's age, tumor size and HBx integration. CONCLUSION: Overexpression of cyclin A occurs in the early stage of HCC carcinogenesis. It may be one of the important approaches by which HBV affects the normal cell cycle of hepatocyte. SN - 0253-3766 UR - https://www.unboundmedicine.com/medline/citation/12408762/Overexpression_of_cyclin_A_in_hepatocellular_carcinoma_and_its_relationship_with_HBx_gene_integration_ L2 - https://medlineplus.gov/livercancer.html DB - PRIME DP - Unbound Medicine ER -