Anti- and pro-oxidant activity of water soluble compounds in Cichorium intybus var. silvestre (Treviso red chicory).J Pharm Biomed Anal. 2002 Nov 07; 30(4):939-45.JP
The anti- and pro-oxidant activity of water soluble components in Cichorium intybus var. silvestre was investigated. This vegetable is domestically known as Treviso red chicory recalling its cultivation in the area of Treviso (Italy). The vegetable juices, obtained by centrifugation of the vegetable and treated at 2 and 102 degrees C, were assessed for their antioxidant activity (AA) using the micellar model system linoleic acid-beta-carotene. The obtained juice at 2 degrees C possessed either anti- or pro-oxidant activity. The boiled juice showed only strong AA, proving that the vegetable pro-oxidant components were thermally instable. Juice components were fractionated by sequential dialysis (1000-300000 Da membrane cut-off), SPE, GFC, and RP-HPLC techniques showing the presence of several highly antioxidant components with different molecular weight (MW), and polar features in Treviso red chicory. The pro-oxidant fraction capable of masking the presence of the antioxidant components in the vegetable juice is retained by a MW>300000 Da dialysis membrane.