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The function of the bcl-x promoter in erythroid progenitor cells.
Blood. 2003 Mar 15; 101(6):2235-42.Blood

Abstract

The protein Bcl-x(L) is essential for survival of erythroid progenitor cells, and it increases substantially during late erythrocyte differentiation due to an increase of mRNA. We mapped the transcription start sites of bcl-x mRNA in mouse and human erythroblasts, and we analyzed the function of the mouse bcl-x promoter by transient and stable transfection assays in a mouse erythroid cell line using plasmids containing the bcl-x promoter fused to a luciferase reporter gene. In mouse erythroblasts, a cluster of start sites at positions -664, -655, and -644 relative to the ATG initiation codon account for almost all transcripts. Human erythroblasts exhibit a start site at -654 that is homologous to the triplet in the mouse. A short sequence element in the mouse bcl-x promoter that includes nucleotides -1804 through -1734 was identified as very important for transcription. This element also showed strong enhancerlike activity in concert with the SV40 promoter in an enhancer test vector. Analyses of mutations indicated that 2 short sequences within the element, about 15 base pair apart, are necessary for full enhancer activity. Gel shift experiments with oligonucleotides representing these sequences revealed specific binding of nuclear proteins from erythroblasts. Some of these proteins are regulated during the late erythroid differentiation.

Authors+Show Affiliations

Veterans Affairs and Vanderbilt University Medical Centers, Nashville, TN 37212, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12411308

Citation

Tian, Cuixia, et al. "The Function of the Bcl-x Promoter in Erythroid Progenitor Cells." Blood, vol. 101, no. 6, 2003, pp. 2235-42.
Tian C, Gregoli P, Bondurant M. The function of the bcl-x promoter in erythroid progenitor cells. Blood. 2003;101(6):2235-42.
Tian, C., Gregoli, P., & Bondurant, M. (2003). The function of the bcl-x promoter in erythroid progenitor cells. Blood, 101(6), 2235-42.
Tian C, Gregoli P, Bondurant M. The Function of the Bcl-x Promoter in Erythroid Progenitor Cells. Blood. 2003 Mar 15;101(6):2235-42. PubMed PMID: 12411308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The function of the bcl-x promoter in erythroid progenitor cells. AU - Tian,Cuixia, AU - Gregoli,Paul, AU - Bondurant,Maurice, Y1 - 2002/10/31/ PY - 2002/11/2/pubmed PY - 2003/4/8/medline PY - 2002/11/2/entrez SP - 2235 EP - 42 JF - Blood JO - Blood VL - 101 IS - 6 N2 - The protein Bcl-x(L) is essential for survival of erythroid progenitor cells, and it increases substantially during late erythrocyte differentiation due to an increase of mRNA. We mapped the transcription start sites of bcl-x mRNA in mouse and human erythroblasts, and we analyzed the function of the mouse bcl-x promoter by transient and stable transfection assays in a mouse erythroid cell line using plasmids containing the bcl-x promoter fused to a luciferase reporter gene. In mouse erythroblasts, a cluster of start sites at positions -664, -655, and -644 relative to the ATG initiation codon account for almost all transcripts. Human erythroblasts exhibit a start site at -654 that is homologous to the triplet in the mouse. A short sequence element in the mouse bcl-x promoter that includes nucleotides -1804 through -1734 was identified as very important for transcription. This element also showed strong enhancerlike activity in concert with the SV40 promoter in an enhancer test vector. Analyses of mutations indicated that 2 short sequences within the element, about 15 base pair apart, are necessary for full enhancer activity. Gel shift experiments with oligonucleotides representing these sequences revealed specific binding of nuclear proteins from erythroblasts. Some of these proteins are regulated during the late erythroid differentiation. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/12411308/The_function_of_the_bcl_x_promoter_in_erythroid_progenitor_cells_ DB - PRIME DP - Unbound Medicine ER -