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Prognostic potential of the telomerase subunit human telomerase reverse transcriptase in tumor tissue and nontumorous mucosa from patients with colorectal carcinoma.
Cancer. 2002 Nov 15; 95(10):2103-11.C

Abstract

BACKGROUND

The stabilization of telomere lengths by telomerase activation is an important step in carcinogenesis and cell immortalization. Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of this enzyme. The objective of this study was to evaluate the use of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for the quantification of hTERT in tumor and nontumorous tissue samples.

METHODS

Matched samples of tumor and adjacent nontumorous mucosa samples from 57 patients with completely resected colorectal carcinoma (International Union Against Cancer Stage I-IV) who underwent complete resection (R0) were quantified for hTERT mRNA expression using real-time RT-PCR. The expression levels were correlated with histopathologic findings and with survival. The median follow-up was 76 months.

RESULTS

hTERT mRNA was expressed in all tumor samples and in all samples of adjacent mucosa. In 12 patients (21%), there was higher hTERT expression in tumor samples compared with nontumorous samples. Compared with tumor samples, the expression of hTERT in samples of nontumorous mucosa decreased with age (P = 0.06). hTERT mRNA expression in both tumor tissue and adjacent mucosa was correlated significantly with the histologic grade of colorectal carcinoma (P < 0.04 and P < 0.05, respectively). Patients with hTERT expression in tumor tissue in relation to the adjacent mucosa of > 0.57 had a significantly poorer overall survival compared with patients with lower hTERT ratios (P < 0.02). In addition to the established prognostic factor lymphatic vessel invasion, the hTERT ratio proved to be of independent prognostic value (P < 0.05).

CONCLUSIONS

The prognostic potential of hTERT in patients with colorectal carcinoma and the correlation of hTERT with tumor grade underlines the role of hTERT as a molecular marker for biologic tumor staging.

Authors+Show Affiliations

Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany. gertler@nt1.chir.med.tu-muenchen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

12412163

Citation

Gertler, Ralf, et al. "Prognostic Potential of the Telomerase Subunit Human Telomerase Reverse Transcriptase in Tumor Tissue and Nontumorous Mucosa From Patients With Colorectal Carcinoma." Cancer, vol. 95, no. 10, 2002, pp. 2103-11.
Gertler R, Rosenberg R, Stricker D, et al. Prognostic potential of the telomerase subunit human telomerase reverse transcriptase in tumor tissue and nontumorous mucosa from patients with colorectal carcinoma. Cancer. 2002;95(10):2103-11.
Gertler, R., Rosenberg, R., Stricker, D., Werner, M., Lassmann, S., Ulm, K., Nekarda, H., & Siewert, J. R. (2002). Prognostic potential of the telomerase subunit human telomerase reverse transcriptase in tumor tissue and nontumorous mucosa from patients with colorectal carcinoma. Cancer, 95(10), 2103-11.
Gertler R, et al. Prognostic Potential of the Telomerase Subunit Human Telomerase Reverse Transcriptase in Tumor Tissue and Nontumorous Mucosa From Patients With Colorectal Carcinoma. Cancer. 2002 Nov 15;95(10):2103-11. PubMed PMID: 12412163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prognostic potential of the telomerase subunit human telomerase reverse transcriptase in tumor tissue and nontumorous mucosa from patients with colorectal carcinoma. AU - Gertler,Ralf, AU - Rosenberg,Robert, AU - Stricker,Dominik, AU - Werner,Martin, AU - Lassmann,Silke, AU - Ulm,Kurt, AU - Nekarda,Hjalmar, AU - Siewert,Joerg-Ruediger, PY - 2002/11/2/pubmed PY - 2003/2/1/medline PY - 2002/11/2/entrez SP - 2103 EP - 11 JF - Cancer JO - Cancer VL - 95 IS - 10 N2 - BACKGROUND: The stabilization of telomere lengths by telomerase activation is an important step in carcinogenesis and cell immortalization. Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of this enzyme. The objective of this study was to evaluate the use of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for the quantification of hTERT in tumor and nontumorous tissue samples. METHODS: Matched samples of tumor and adjacent nontumorous mucosa samples from 57 patients with completely resected colorectal carcinoma (International Union Against Cancer Stage I-IV) who underwent complete resection (R0) were quantified for hTERT mRNA expression using real-time RT-PCR. The expression levels were correlated with histopathologic findings and with survival. The median follow-up was 76 months. RESULTS: hTERT mRNA was expressed in all tumor samples and in all samples of adjacent mucosa. In 12 patients (21%), there was higher hTERT expression in tumor samples compared with nontumorous samples. Compared with tumor samples, the expression of hTERT in samples of nontumorous mucosa decreased with age (P = 0.06). hTERT mRNA expression in both tumor tissue and adjacent mucosa was correlated significantly with the histologic grade of colorectal carcinoma (P < 0.04 and P < 0.05, respectively). Patients with hTERT expression in tumor tissue in relation to the adjacent mucosa of > 0.57 had a significantly poorer overall survival compared with patients with lower hTERT ratios (P < 0.02). In addition to the established prognostic factor lymphatic vessel invasion, the hTERT ratio proved to be of independent prognostic value (P < 0.05). CONCLUSIONS: The prognostic potential of hTERT in patients with colorectal carcinoma and the correlation of hTERT with tumor grade underlines the role of hTERT as a molecular marker for biologic tumor staging. SN - 0008-543X UR - https://www.unboundmedicine.com/medline/citation/12412163/Prognostic_potential_of_the_telomerase_subunit_human_telomerase_reverse_transcriptase_in_tumor_tissue_and_nontumorous_mucosa_from_patients_with_colorectal_carcinoma_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0008-543X&amp;date=2002&amp;volume=95&amp;issue=10&amp;spage=2103 DB - PRIME DP - Unbound Medicine ER -