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Antibody response to phenolic glycolipid I and Mycobacterium w antigens and its relation to bacterial load in M. leprae-infected mice and leprosy patients.
Clin Exp Immunol. 1989 Nov; 78(2):214-8.CE

Abstract

Twenty-six inbred BALB/cBy mice were infected with live Mycobacterium leprae by injecting 6 x 10(3) bacilli in the hind footpad. Bleeds were collected at monthly intervals. After 6 months, acid-fast bacilli (AFB) were harvested monthly from the footpad of mice. The sera were analysed in enzyme immunoassay for antibodies against phenolic glycolipid I (PGL-I) of M. leprae and antigens of Mycobacterium w (M. w); 21 out of 26 (80.7%) mice demonstrated the presence of antibodies against PGL-I and M. w . Anti-M. w antibodies appeared slightly earlier than did anti-PGL-I antibodies. The titre of anti-M. w antibodies was higher than that of anti-PGL-I antibodies. The mice giving a positive antibody response had more than 7 x 10(5) AFB/footpad. The coefficient of correlation (r) between the number of AFB and antibody titres at the time of harvest was 0.566 for PGL-I and 0.628 for M. w. The value of r for bacterial index and antibody titres in 188 leprosy patients was 0.510 for PGL-I and 0.418 for M. w; these values were statistically significant (P < 0.001). The decrease in bacterial index and antibody titres in treated lepromatous leprosy patients correlated with increase in the duration of chemotherapy. The measurement of anti-PGL-I antibodies of IgM class may serve as an adjunct to skin biopsy and skin-slit smear for serial monitoring of the bacterial load in the course of chemotherapy in leprosy control programmes.

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Authors+Show Affiliations

Moudgil KD
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Gupta SK
No affiliation info available
Naraynan PR
No affiliation info available
Srivastava LM
No affiliation info available
Mishra RS
No affiliation info available
Talwar GP
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntigens, BacterialCase-Control StudiesCross ReactionsGlycolipidsHumansImmunoglobulin MLeprosyMiceMice, Inbred BALB CMycobacteriumMycobacterium leprae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12412751

Citation

Moudgil, K D., et al. "Antibody Response to Phenolic Glycolipid I and Mycobacterium W Antigens and Its Relation to Bacterial Load in M. Leprae-infected Mice and Leprosy Patients." Clinical and Experimental Immunology, vol. 78, no. 2, 1989, pp. 214-8.
Moudgil KD, Gupta SK, Naraynan PR, et al. Antibody response to phenolic glycolipid I and Mycobacterium w antigens and its relation to bacterial load in M. leprae-infected mice and leprosy patients. Clin Exp Immunol. 1989;78(2):214-8.
Moudgil, K. D., Gupta, S. K., Naraynan, P. R., Srivastava, L. M., Mishra, R. S., & Talwar, G. P. (1989). Antibody response to phenolic glycolipid I and Mycobacterium w antigens and its relation to bacterial load in M. leprae-infected mice and leprosy patients. Clinical and Experimental Immunology, 78(2), 214-8.
Moudgil KD, et al. Antibody Response to Phenolic Glycolipid I and Mycobacterium W Antigens and Its Relation to Bacterial Load in M. Leprae-infected Mice and Leprosy Patients. Clin Exp Immunol. 1989;78(2):214-8. PubMed PMID: 12412751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibody response to phenolic glycolipid I and Mycobacterium w antigens and its relation to bacterial load in M. leprae-infected mice and leprosy patients. AU - Moudgil,K D, AU - Gupta,S K, AU - Naraynan,P R, AU - Srivastava,L M, AU - Mishra,R S, AU - Talwar,G P, PY - 1989/11/1/pubmed PY - 2002/11/28/medline PY - 1989/11/1/entrez SP - 214 EP - 8 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 78 IS - 2 N2 - Twenty-six inbred BALB/cBy mice were infected with live Mycobacterium leprae by injecting 6 x 10(3) bacilli in the hind footpad. Bleeds were collected at monthly intervals. After 6 months, acid-fast bacilli (AFB) were harvested monthly from the footpad of mice. The sera were analysed in enzyme immunoassay for antibodies against phenolic glycolipid I (PGL-I) of M. leprae and antigens of Mycobacterium w (M. w); 21 out of 26 (80.7%) mice demonstrated the presence of antibodies against PGL-I and M. w . Anti-M. w antibodies appeared slightly earlier than did anti-PGL-I antibodies. The titre of anti-M. w antibodies was higher than that of anti-PGL-I antibodies. The mice giving a positive antibody response had more than 7 x 10(5) AFB/footpad. The coefficient of correlation (r) between the number of AFB and antibody titres at the time of harvest was 0.566 for PGL-I and 0.628 for M. w. The value of r for bacterial index and antibody titres in 188 leprosy patients was 0.510 for PGL-I and 0.418 for M. w; these values were statistically significant (P < 0.001). The decrease in bacterial index and antibody titres in treated lepromatous leprosy patients correlated with increase in the duration of chemotherapy. The measurement of anti-PGL-I antibodies of IgM class may serve as an adjunct to skin biopsy and skin-slit smear for serial monitoring of the bacterial load in the course of chemotherapy in leprosy control programmes. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/12412751/Antibody_response_to_phenolic_glycolipid_I_and_Mycobacterium_w_antigens_and_its_relation_to_bacterial_load_in_M__leprae_infected_mice_and_leprosy_patients_ DB - PRIME DP - Unbound Medicine ER -