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Capillary electrophoresis of baclofen with argon-ion laser-induced fluorescence detection.
J Chromatogr A. 2002 Oct 04; 972(2):289-93.JC

Abstract

A capillary electrophoretic method with laser-induced fluorescence detection for baclofen (4-amino-3-p-chlorophenylbutyric acid) has been developed. 6-Carboxyfluorescein succinimidyl ester was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the lowest derivatizable concentration limit for baclofen in aqueous solution was 10 nM (2 ng baclofen/ml). Coupled with a simple clean up procedure, the method can be applied to the analysis of baclofen in human plasma at micromolar level. Recovery of spiked baclofen in plasma was 95%. The relative standard deviation values on peak size (0.5 microM level) and migration time were 8.2 and 1.0% (n=7), respectively. The limit of detection of baclofen in plasma was 0.1 microM (21 ng/ml).

Authors+Show Affiliations

Department of Chemistry, Tunghai University, Taichung, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12416888

Citation

Gu, Yi-Sheng, and Chen-Wen Whang. "Capillary Electrophoresis of Baclofen With Argon-ion Laser-induced Fluorescence Detection." Journal of Chromatography. A, vol. 972, no. 2, 2002, pp. 289-93.
Gu YS, Whang CW. Capillary electrophoresis of baclofen with argon-ion laser-induced fluorescence detection. J Chromatogr A. 2002;972(2):289-93.
Gu, Y. S., & Whang, C. W. (2002). Capillary electrophoresis of baclofen with argon-ion laser-induced fluorescence detection. Journal of Chromatography. A, 972(2), 289-93.
Gu YS, Whang CW. Capillary Electrophoresis of Baclofen With Argon-ion Laser-induced Fluorescence Detection. J Chromatogr A. 2002 Oct 4;972(2):289-93. PubMed PMID: 12416888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis of baclofen with argon-ion laser-induced fluorescence detection. AU - Gu,Yi-Sheng, AU - Whang,Chen-Wen, PY - 2002/11/6/pubmed PY - 2003/3/28/medline PY - 2002/11/6/entrez SP - 289 EP - 93 JF - Journal of chromatography. A JO - J Chromatogr A VL - 972 IS - 2 N2 - A capillary electrophoretic method with laser-induced fluorescence detection for baclofen (4-amino-3-p-chlorophenylbutyric acid) has been developed. 6-Carboxyfluorescein succinimidyl ester was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the lowest derivatizable concentration limit for baclofen in aqueous solution was 10 nM (2 ng baclofen/ml). Coupled with a simple clean up procedure, the method can be applied to the analysis of baclofen in human plasma at micromolar level. Recovery of spiked baclofen in plasma was 95%. The relative standard deviation values on peak size (0.5 microM level) and migration time were 8.2 and 1.0% (n=7), respectively. The limit of detection of baclofen in plasma was 0.1 microM (21 ng/ml). SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/12416888/Capillary_electrophoresis_of_baclofen_with_argon_ion_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -