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Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine.
Arch Microbiol. 2002 Dec; 178(6):411-20.AM

Abstract

Over 2,800 Tn 5 insertion mutants of Salmonella enterica sv. Typhimurium were screened for the loss of ability to suppress the multiplication of a spectinomycin-resistant (Spc(r)) but otherwise isogenic S. enterica sv. Typhimurium strain, when the Spc(r) mutant was added to 24-h LB broth cultures of the mutants. Selected "growth non-suppressive" (GNS) mutants were defective in respiration (insertions in arcA and fnr), amino acid biosynthesis (aroA and aroD), nutrient uptake and its regulation (tdcC and crp), and chemotaxis (fliD). In the last GNS mutant, the transposon inactivated yhjH, an ORF with unknown function which shows sequence similarity to di-guanylate cyclase and to novel two-component signal transduction proteins. In newly hatched chickens, all of the mutants, with the exception of the fliDmutant, were also unable to suppress colonization of the alimentary tract by the parent strain inoculated 1 day later. Defined mutations in luxS or sdiA,genes which contribute to quorum sensing in S. enterica sv. Typhimurium, had no effect on the stationary-phase growth suppression. Analysis of a transcriptional fusion construct indicated that yhjH was moderately expressed in the exponential phase of growth and up-regulated upon entry into stationary phase. Expression of yhjH was also considerably suppressed by the addition of supernatant from a 24-h stationary-phase S. enterica sv. Typhimurium culture, suggesting that the gene belongs to a new sensing and signaling regulatory pathway in S. enterica sv. Typhimurium.

Authors+Show Affiliations

Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12420160

Citation

Rychlik, Ivan, et al. "Identification of Salmonella Enterica Serovar Typhimurium Genes Associated With Growth Suppression in Stationary-phase Nutrient Broth Cultures and in the Chicken Intestine." Archives of Microbiology, vol. 178, no. 6, 2002, pp. 411-20.
Rychlik I, Martin G, Methner U, et al. Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine. Arch Microbiol. 2002;178(6):411-20.
Rychlik, I., Martin, G., Methner, U., Lovell, M., Cardova, L., Sebkova, A., Sevcik, M., Damborsky, J., & Barrow, P. A. (2002). Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine. Archives of Microbiology, 178(6), 411-20.
Rychlik I, et al. Identification of Salmonella Enterica Serovar Typhimurium Genes Associated With Growth Suppression in Stationary-phase Nutrient Broth Cultures and in the Chicken Intestine. Arch Microbiol. 2002;178(6):411-20. PubMed PMID: 12420160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine. AU - Rychlik,Ivan, AU - Martin,Gerald, AU - Methner,Ulrich, AU - Lovell,Margaret, AU - Cardova,Lenka, AU - Sebkova,Alena, AU - Sevcik,Mojmor, AU - Damborsky,Jiri, AU - Barrow,Paul A, Y1 - 2002/09/19/ PY - 2001/11/30/received PY - 2002/05/13/revised PY - 2002/06/07/accepted PY - 2002/11/7/pubmed PY - 2003/6/11/medline PY - 2002/11/7/entrez SP - 411 EP - 20 JF - Archives of microbiology JO - Arch Microbiol VL - 178 IS - 6 N2 - Over 2,800 Tn 5 insertion mutants of Salmonella enterica sv. Typhimurium were screened for the loss of ability to suppress the multiplication of a spectinomycin-resistant (Spc(r)) but otherwise isogenic S. enterica sv. Typhimurium strain, when the Spc(r) mutant was added to 24-h LB broth cultures of the mutants. Selected "growth non-suppressive" (GNS) mutants were defective in respiration (insertions in arcA and fnr), amino acid biosynthesis (aroA and aroD), nutrient uptake and its regulation (tdcC and crp), and chemotaxis (fliD). In the last GNS mutant, the transposon inactivated yhjH, an ORF with unknown function which shows sequence similarity to di-guanylate cyclase and to novel two-component signal transduction proteins. In newly hatched chickens, all of the mutants, with the exception of the fliDmutant, were also unable to suppress colonization of the alimentary tract by the parent strain inoculated 1 day later. Defined mutations in luxS or sdiA,genes which contribute to quorum sensing in S. enterica sv. Typhimurium, had no effect on the stationary-phase growth suppression. Analysis of a transcriptional fusion construct indicated that yhjH was moderately expressed in the exponential phase of growth and up-regulated upon entry into stationary phase. Expression of yhjH was also considerably suppressed by the addition of supernatant from a 24-h stationary-phase S. enterica sv. Typhimurium culture, suggesting that the gene belongs to a new sensing and signaling regulatory pathway in S. enterica sv. Typhimurium. SN - 0302-8933 UR - https://www.unboundmedicine.com/medline/citation/12420160/Identification_of_Salmonella_enterica_serovar_Typhimurium_genes_associated_with_growth_suppression_in_stationary_phase_nutrient_broth_cultures_and_in_the_chicken_intestine_ L2 - https://dx.doi.org/10.1007/s00203-002-0458-7 DB - PRIME DP - Unbound Medicine ER -