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Tissue transglutaminase antibodies in patients with end-stage heart failure.
Am J Gastroenterol 2002; 97(11):2850-4AJ

Abstract

OBJECTIVE

For celiac disease (CD), screening a trend has recently emerged to measure tissue transglutaminase antibodies (tTGA) by immunoassays instead of the more laborious endomysial antibodies (EmA), as they recognize the same target, tissue transglutaminase (tTG). However, a high rate of false-positive results has been reported in some patient series with diseases known to be associated with CD. Moreover, tTG is a ubiquitous, multifunctional enzyme, overexpressed in experimental models of heart failure. Therefore, we assessed the specificity of tTGA assays in a large series of EmA-negative patients with end-stage heart failure.

METHODS

We studied 288 patients with end-stage heart failure and 60 blood donors. No subject had clinical evidence of CD or IgA deficiency, and all were EmA negative. Serum IgA and IgG tTGA were measured by means of commercial kits using as substrate, either guinea pig or recombinant human tTG. Blocking studies and Western blots were also performed using recombinant human tTG.

RESULTS

All blood donor sera were IgA tTGA negative. IgA tTGA positivity was observed in 47.6% and 49.1% of patients with heart failure using, respectively, guinea pig tTG and recombinant human tTG as substrates. Preincubation of positive sera with recombinant human tTG resulted in 81% blocking of IgA tTGA in immunoassay. Western blot analysis confirmed the presence of antibodies against recombinant human tTG. IgA tTGA-positive sera were also IgG tTGA positive.

CONCLUSIONS

IgA and IgG tTGA occur in a large number of EmA-negative patients with end-stage heart failure, and their presence is unlikely to be caused by concomitant CD.

Authors+Show Affiliations

IRCCS-Ospedale Maggiore, University of Milan, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12425559

Citation

Peracchi, Maddalena, et al. "Tissue Transglutaminase Antibodies in Patients With End-stage Heart Failure." The American Journal of Gastroenterology, vol. 97, no. 11, 2002, pp. 2850-4.
Peracchi M, Trovato C, Longhi M, et al. Tissue transglutaminase antibodies in patients with end-stage heart failure. Am J Gastroenterol. 2002;97(11):2850-4.
Peracchi, M., Trovato, C., Longhi, M., Gasparin, M., Conte, D., Tarantino, C., ... Bardella, M. T. (2002). Tissue transglutaminase antibodies in patients with end-stage heart failure. The American Journal of Gastroenterology, 97(11), pp. 2850-4.
Peracchi M, et al. Tissue Transglutaminase Antibodies in Patients With End-stage Heart Failure. Am J Gastroenterol. 2002;97(11):2850-4. PubMed PMID: 12425559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue transglutaminase antibodies in patients with end-stage heart failure. AU - Peracchi,Maddalena, AU - Trovato,Cristina, AU - Longhi,Massimo, AU - Gasparin,Maurizio, AU - Conte,Dario, AU - Tarantino,Cristina, AU - Prati,Daniele, AU - Bardella,Maria Teresa, PY - 2002/11/12/pubmed PY - 2002/11/26/medline PY - 2002/11/12/entrez SP - 2850 EP - 4 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 97 IS - 11 N2 - OBJECTIVE: For celiac disease (CD), screening a trend has recently emerged to measure tissue transglutaminase antibodies (tTGA) by immunoassays instead of the more laborious endomysial antibodies (EmA), as they recognize the same target, tissue transglutaminase (tTG). However, a high rate of false-positive results has been reported in some patient series with diseases known to be associated with CD. Moreover, tTG is a ubiquitous, multifunctional enzyme, overexpressed in experimental models of heart failure. Therefore, we assessed the specificity of tTGA assays in a large series of EmA-negative patients with end-stage heart failure. METHODS: We studied 288 patients with end-stage heart failure and 60 blood donors. No subject had clinical evidence of CD or IgA deficiency, and all were EmA negative. Serum IgA and IgG tTGA were measured by means of commercial kits using as substrate, either guinea pig or recombinant human tTG. Blocking studies and Western blots were also performed using recombinant human tTG. RESULTS: All blood donor sera were IgA tTGA negative. IgA tTGA positivity was observed in 47.6% and 49.1% of patients with heart failure using, respectively, guinea pig tTG and recombinant human tTG as substrates. Preincubation of positive sera with recombinant human tTG resulted in 81% blocking of IgA tTGA in immunoassay. Western blot analysis confirmed the presence of antibodies against recombinant human tTG. IgA tTGA-positive sera were also IgG tTGA positive. CONCLUSIONS: IgA and IgG tTGA occur in a large number of EmA-negative patients with end-stage heart failure, and their presence is unlikely to be caused by concomitant CD. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/12425559/Tissue_transglutaminase_antibodies_in_patients_with_end_stage_heart_failure_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0002-9270&date=2002&volume=97&issue=11&spage=2850 DB - PRIME DP - Unbound Medicine ER -