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A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana.
Planta. 2002 Nov; 216(1):44-56.P

Abstract

A highly sensitive and accurate multiplex gas chromatography-tandem mass spectrometry (GC-MS/MS) technique is reported for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid. The optimized setup allows the routine processing and analysis of up to 60 plant samples of between 20 and 200 mg of fresh weight per day. The protocol was designed and the equipment used was chosen to facilitate implementation of the method into other laboratories and to provide access to state-of-the-art analytical tools for the acidic phytohormones and related signalling molecules. Whole-plant organ-distribution maps for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid were generated for Arabidopsis thaliana (L.) Heynh. For leaves of A. thaliana, a spatial resolution of hormone quantitation down to approximately 2 mm(2) was achieved.

Authors+Show Affiliations

Müller A
Lehrstuhl für Pflanzenphysiologie, Fakultät für Biologie, Ruhr-Universität Bochum, 44780 Bochum, Germany.
Düchting P
No affiliation info available
Weiler EW
No affiliation info available

MeSH

Abscisic AcidAlgorithmsArabidopsisBiological TransportCyclopentanesFatty Acids, UnsaturatedGas Chromatography-Mass SpectrometryIndoleacetic AcidsMolecular StructureOxylipinsPlant Growth RegulatorsSalicylic AcidSignal Transduction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12430013

Citation

Müller, Axel, et al. "A Multiplex GC-MS/MS Technique for the Sensitive and Quantitative Single-run Analysis of Acidic Phytohormones and Related Compounds, and Its Application to Arabidopsis Thaliana." Planta, vol. 216, no. 1, 2002, pp. 44-56.
Müller A, Düchting P, Weiler EW. A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana. Planta. 2002;216(1):44-56.
Müller, A., Düchting, P., & Weiler, E. W. (2002). A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana. Planta, 216(1), 44-56.
Müller A, Düchting P, Weiler EW. A Multiplex GC-MS/MS Technique for the Sensitive and Quantitative Single-run Analysis of Acidic Phytohormones and Related Compounds, and Its Application to Arabidopsis Thaliana. Planta. 2002;216(1):44-56. PubMed PMID: 12430013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana. AU - Müller,Axel, AU - Düchting,Petra, AU - Weiler,Elmar W, Y1 - 2002/11/12/ PY - 2002/04/03/received PY - 2002/07/02/accepted PY - 2002/11/14/pubmed PY - 2003/3/26/medline PY - 2002/11/14/entrez SP - 44 EP - 56 JF - Planta JO - Planta VL - 216 IS - 1 N2 - A highly sensitive and accurate multiplex gas chromatography-tandem mass spectrometry (GC-MS/MS) technique is reported for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid. The optimized setup allows the routine processing and analysis of up to 60 plant samples of between 20 and 200 mg of fresh weight per day. The protocol was designed and the equipment used was chosen to facilitate implementation of the method into other laboratories and to provide access to state-of-the-art analytical tools for the acidic phytohormones and related signalling molecules. Whole-plant organ-distribution maps for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid were generated for Arabidopsis thaliana (L.) Heynh. For leaves of A. thaliana, a spatial resolution of hormone quantitation down to approximately 2 mm(2) was achieved. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/12430013/A_multiplex_GC_MS/MS_technique_for_the_sensitive_and_quantitative_single_run_analysis_of_acidic_phytohormones_and_related_compounds_and_its_application_to_Arabidopsis_thaliana_ DB - PRIME DP - Unbound Medicine ER -