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Nitric oxide synthase II in rat skeletal muscles.
Histochem Cell Biol. 2002 Nov; 118(5):371-9.HC

Abstract

Constitutive expression of nitric oxide synthase (NOS) II was found in rat hindlimb muscles by immunohistochemistry and western blotting during development from embryonic day 21 to the adult stage of 75 days. The immunohistochemical NOS II expression pattern was related to the physiological metabolic fibre types SO (slow-oxidative), FOG I, II (fast-oxidative glycolytic; I more glycolytic, II more oxidative) and FG (fast-glycolytic) and to the myosin-based fibre types I and IIA, IIB (IIX not separated) identified in serial sections by enzyme histochemistry and immunohistochemistry. In adult muscles only the small population of FOG II fibres, which is a part of both IIA and IIB fibre population, showed NOS II immunoreactivity. This is the reason that only weak NOS II expression in adult hindlimb muscles has been detected by western blotting. Hindlimb muscles of embryonic, neonatal and young rats of 8 days expressed more NOS II as compared with adult rat hindlimb muscles. This can be explained by the findings that before the age of 21 days fast fibres were metabolically undifferentiated, all of them were NOS II positive and contribute to the NOS II expression of the muscle. In muscles of diabetic rats the NOS II expression was elevated indicating an inhibition of glucose uptake into the muscle fibres of diabetic muscles. Our findings suggest that the NOS II may be designated both as constitutive and inducible.

Authors+Show Affiliations

Institute of Anatomy, University of Leipzig, Liebigstrasse 13, 04103 Leipzig, Germany. punktk@medizin.uni-leipzig.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12432448

Citation

Punkt, Karla, et al. "Nitric Oxide Synthase II in Rat Skeletal Muscles." Histochemistry and Cell Biology, vol. 118, no. 5, 2002, pp. 371-9.
Punkt K, Naupert A, Wellner M, et al. Nitric oxide synthase II in rat skeletal muscles. Histochem Cell Biol. 2002;118(5):371-9.
Punkt, K., Naupert, A., Wellner, M., Asmussen, G., Schmidt, C., & Buchwalow, I. B. (2002). Nitric oxide synthase II in rat skeletal muscles. Histochemistry and Cell Biology, 118(5), 371-9.
Punkt K, et al. Nitric Oxide Synthase II in Rat Skeletal Muscles. Histochem Cell Biol. 2002;118(5):371-9. PubMed PMID: 12432448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide synthase II in rat skeletal muscles. AU - Punkt,Karla, AU - Naupert,Andreas, AU - Wellner,Maren, AU - Asmussen,Gerhard, AU - Schmidt,Christine, AU - Buchwalow,Igor B, Y1 - 2002/10/16/ PY - 2002/09/17/accepted PY - 2002/11/15/pubmed PY - 2003/6/24/medline PY - 2002/11/15/entrez SP - 371 EP - 9 JF - Histochemistry and cell biology JO - Histochem Cell Biol VL - 118 IS - 5 N2 - Constitutive expression of nitric oxide synthase (NOS) II was found in rat hindlimb muscles by immunohistochemistry and western blotting during development from embryonic day 21 to the adult stage of 75 days. The immunohistochemical NOS II expression pattern was related to the physiological metabolic fibre types SO (slow-oxidative), FOG I, II (fast-oxidative glycolytic; I more glycolytic, II more oxidative) and FG (fast-glycolytic) and to the myosin-based fibre types I and IIA, IIB (IIX not separated) identified in serial sections by enzyme histochemistry and immunohistochemistry. In adult muscles only the small population of FOG II fibres, which is a part of both IIA and IIB fibre population, showed NOS II immunoreactivity. This is the reason that only weak NOS II expression in adult hindlimb muscles has been detected by western blotting. Hindlimb muscles of embryonic, neonatal and young rats of 8 days expressed more NOS II as compared with adult rat hindlimb muscles. This can be explained by the findings that before the age of 21 days fast fibres were metabolically undifferentiated, all of them were NOS II positive and contribute to the NOS II expression of the muscle. In muscles of diabetic rats the NOS II expression was elevated indicating an inhibition of glucose uptake into the muscle fibres of diabetic muscles. Our findings suggest that the NOS II may be designated both as constitutive and inducible. SN - 0948-6143 UR - https://www.unboundmedicine.com/medline/citation/12432448/Nitric_oxide_synthase_II_in_rat_skeletal_muscles_ L2 - https://dx.doi.org/10.1007/s00418-002-0465-4 DB - PRIME DP - Unbound Medicine ER -