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Antibodies from dengue patient sera cross-react with endothelial cells and induce damage.
J Med Virol. 2003 Jan; 69(1):82-90.JM

Abstract

Dengue virus infection causes a wide range of diseases from the mild febrile illness dengue fever to the life-threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Vascular leakage and hemorrhagic syndrome are the clinical features associated with dengue infection, yet the mechanisms remain unclear. In this study, the cross-reactivity of dengue patient sera with endothelial cells was demonstrated. There were higher percentages of endothelial cells reactive with dengue hemorrhagic fever/dengue shock syndrome patient sera than those with dengue fever patient sera. The percentages of endothelial cells reactive with patient serum IgM were higher than those with IgG. Further studies showed that the endothelial cell binding activity was inhibited by pretreatment with dengue virus nonstructural protein 1 (NS1). The antibodies against NS1 produced after dengue virus infection may, at least in part, account for the cross-reactivity of patient sera with endothelial cells. Furthermore, dengue patient sera induced endothelial cell apoptosis via a caspase-dependent pathway that was also inhibited by NS1 pretreatment. In addition to apoptosis, patient sera caused cell lysis in the presence of complement, and DHF/DSS patient sera showed higher percentages of cytotoxicity than dengue fever patient sera. Thus, the generation of cross-reactive autoantibodies against endothelial cells would lead to their dysfunction, which may play a role in the pathogenesis of dengue virus infection.

Authors+Show Affiliations

Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12436482

Citation

Lin, Chiou-Feng, et al. "Antibodies From Dengue Patient Sera Cross-react With Endothelial Cells and Induce Damage." Journal of Medical Virology, vol. 69, no. 1, 2003, pp. 82-90.
Lin CF, Lei HY, Shiau AL, et al. Antibodies from dengue patient sera cross-react with endothelial cells and induce damage. J Med Virol. 2003;69(1):82-90.
Lin, C. F., Lei, H. Y., Shiau, A. L., Liu, C. C., Liu, H. S., Yeh, T. M., Chen, S. H., & Lin, Y. S. (2003). Antibodies from dengue patient sera cross-react with endothelial cells and induce damage. Journal of Medical Virology, 69(1), 82-90.
Lin CF, et al. Antibodies From Dengue Patient Sera Cross-react With Endothelial Cells and Induce Damage. J Med Virol. 2003;69(1):82-90. PubMed PMID: 12436482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibodies from dengue patient sera cross-react with endothelial cells and induce damage. AU - Lin,Chiou-Feng, AU - Lei,Huan-Yao, AU - Shiau,Ai-Li, AU - Liu,Ching-Chuan, AU - Liu,Hsiao-Sheng, AU - Yeh,Trai-Ming, AU - Chen,Shun-Hua, AU - Lin,Yee-Shin, PY - 2002/11/19/pubmed PY - 2003/3/26/medline PY - 2002/11/19/entrez SP - 82 EP - 90 JF - Journal of medical virology JO - J Med Virol VL - 69 IS - 1 N2 - Dengue virus infection causes a wide range of diseases from the mild febrile illness dengue fever to the life-threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Vascular leakage and hemorrhagic syndrome are the clinical features associated with dengue infection, yet the mechanisms remain unclear. In this study, the cross-reactivity of dengue patient sera with endothelial cells was demonstrated. There were higher percentages of endothelial cells reactive with dengue hemorrhagic fever/dengue shock syndrome patient sera than those with dengue fever patient sera. The percentages of endothelial cells reactive with patient serum IgM were higher than those with IgG. Further studies showed that the endothelial cell binding activity was inhibited by pretreatment with dengue virus nonstructural protein 1 (NS1). The antibodies against NS1 produced after dengue virus infection may, at least in part, account for the cross-reactivity of patient sera with endothelial cells. Furthermore, dengue patient sera induced endothelial cell apoptosis via a caspase-dependent pathway that was also inhibited by NS1 pretreatment. In addition to apoptosis, patient sera caused cell lysis in the presence of complement, and DHF/DSS patient sera showed higher percentages of cytotoxicity than dengue fever patient sera. Thus, the generation of cross-reactive autoantibodies against endothelial cells would lead to their dysfunction, which may play a role in the pathogenesis of dengue virus infection. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/12436482/Antibodies_from_dengue_patient_sera_cross_react_with_endothelial_cells_and_induce_damage_ L2 - https://doi.org/10.1002/jmv.10261 DB - PRIME DP - Unbound Medicine ER -