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HLA-DR and ICAM-1 expression and modulation in epithelial cells from nasal polyps.
Laryngoscope. 2002 Nov; 112(11):2067-75.L

Abstract

OBJECTIVE/HYPOTHESIS

Through human leukocyte antigen-DR (HLA-DR) and intercellular adhesion molecule-1 (ICAM-1) expression, nasal epithelial cells could actively participate in the chronic inflammation and eosinophil infiltration observed in nasal polyps. The objective of the study was to evaluate HLA-DR and ICAM-1 expression in polyp epithelium and in a culture model of polyp epithelial cells allowing ciliated and secretory differentiation.

STUDY DESIGN

Prospective non-randomized controlled in vitro study.

METHODS

The in vitro HLA-DR and ICAM-1 expression was studied under basal conditions or after exposure to interferon-gamma, transforming growth factor-beta1, lipopolysaccharide, dexamethasone, or cetirizine. HLA-DR and ICAM-1 expression was investigated in situ by immunohistochemical staining of polyps and in vitro by immunofluorescent staining of cell cultures. HLA-DR and ICAM-1 were localized in cultured cells by confocal microscopy. Cultured cells expressing HLA-DR and ICAM-1 were quantified by flow cytometry.

RESULTS

Both HLA-DR and ICAM-1 showed significant immunostaining of nasal polyp epithelium. In nasal polyp epithelial cell cultures, less than 5% of cells were positive for HLA-DR whereas 40% were positive for ICAM-1 at day 3. In vitro, HLA-DR was mainly located in the cytoplasm and ICAM-1 predominated on the apicolateral cytoplasmic membrane. Comparison of in situ and in vitro results showed that well-differentiated and poorly differentiated cells predominantly expressed HLA-DR and ICAM-1, respectively. Interferon-gamma significantly increased HLA-DR and ICAM-1 expression, whereas transforming growth factor-beta1 significantly decreased HLA-DR expression and lipopolysaccharide significantly increased ICAM-1 expression.

CONCLUSION

HLA-DR and ICAM-1 epithelial expression in nasal polyps in situ and in vitro and their in vitro modulation reinforce the active role of epithelial cells in chronic inflammatory diseases of the upper airways.

Authors+Show Affiliations

Institut National de la Santé et de la Recherche Médicale, Unité U492, Faculté de Médecine, Paris XII, Créteil, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12439183

Citation

Papon, Jean-François, et al. "HLA-DR and ICAM-1 Expression and Modulation in Epithelial Cells From Nasal Polyps." The Laryngoscope, vol. 112, no. 11, 2002, pp. 2067-75.
Papon JF, Coste A, Gendron MC, et al. HLA-DR and ICAM-1 expression and modulation in epithelial cells from nasal polyps. Laryngoscope. 2002;112(11):2067-75.
Papon, J. F., Coste, A., Gendron, M. C., Cordonnier, C., Wingerstmann, L., Peynègre, R., & Escudier, E. (2002). HLA-DR and ICAM-1 expression and modulation in epithelial cells from nasal polyps. The Laryngoscope, 112(11), 2067-75.
Papon JF, et al. HLA-DR and ICAM-1 Expression and Modulation in Epithelial Cells From Nasal Polyps. Laryngoscope. 2002;112(11):2067-75. PubMed PMID: 12439183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HLA-DR and ICAM-1 expression and modulation in epithelial cells from nasal polyps. AU - Papon,Jean-François, AU - Coste,André, AU - Gendron,Marie-Claude, AU - Cordonnier,Catherine, AU - Wingerstmann,Laure, AU - Peynègre,Roger, AU - Escudier,Estelle, PY - 2002/11/20/pubmed PY - 2002/12/18/medline PY - 2002/11/20/entrez SP - 2067 EP - 75 JF - The Laryngoscope JO - Laryngoscope VL - 112 IS - 11 N2 - OBJECTIVE/HYPOTHESIS: Through human leukocyte antigen-DR (HLA-DR) and intercellular adhesion molecule-1 (ICAM-1) expression, nasal epithelial cells could actively participate in the chronic inflammation and eosinophil infiltration observed in nasal polyps. The objective of the study was to evaluate HLA-DR and ICAM-1 expression in polyp epithelium and in a culture model of polyp epithelial cells allowing ciliated and secretory differentiation. STUDY DESIGN: Prospective non-randomized controlled in vitro study. METHODS: The in vitro HLA-DR and ICAM-1 expression was studied under basal conditions or after exposure to interferon-gamma, transforming growth factor-beta1, lipopolysaccharide, dexamethasone, or cetirizine. HLA-DR and ICAM-1 expression was investigated in situ by immunohistochemical staining of polyps and in vitro by immunofluorescent staining of cell cultures. HLA-DR and ICAM-1 were localized in cultured cells by confocal microscopy. Cultured cells expressing HLA-DR and ICAM-1 were quantified by flow cytometry. RESULTS: Both HLA-DR and ICAM-1 showed significant immunostaining of nasal polyp epithelium. In nasal polyp epithelial cell cultures, less than 5% of cells were positive for HLA-DR whereas 40% were positive for ICAM-1 at day 3. In vitro, HLA-DR was mainly located in the cytoplasm and ICAM-1 predominated on the apicolateral cytoplasmic membrane. Comparison of in situ and in vitro results showed that well-differentiated and poorly differentiated cells predominantly expressed HLA-DR and ICAM-1, respectively. Interferon-gamma significantly increased HLA-DR and ICAM-1 expression, whereas transforming growth factor-beta1 significantly decreased HLA-DR expression and lipopolysaccharide significantly increased ICAM-1 expression. CONCLUSION: HLA-DR and ICAM-1 epithelial expression in nasal polyps in situ and in vitro and their in vitro modulation reinforce the active role of epithelial cells in chronic inflammatory diseases of the upper airways. SN - 0023-852X UR - https://www.unboundmedicine.com/medline/citation/12439183/HLA_DR_and_ICAM_1_expression_and_modulation_in_epithelial_cells_from_nasal_polyps_ L2 - https://doi.org/10.1097/00005537-200211000-00030 DB - PRIME DP - Unbound Medicine ER -