Tags

Type your tag names separated by a space and hit enter

Decreased expression of membrane IL-5 receptor alpha on human eosinophils: I. Loss of membrane IL-5 receptor alpha on airway eosinophils and increased soluble IL-5 receptor alpha in the airway after allergen challenge.
J Immunol. 2002 Dec 01; 169(11):6452-8.JI

Abstract

IL-5 is a key cytokine for eosinophil maturation, recruitment, activation, and possibly the development of inflammation in asthma. High concentrations of IL-5 are present in the airway after Ag challenge, but the responsiveness of airway eosinophils to IL-5 is not well characterized. The objectives of this study were to establish, following airway Ag challenge: 1) the expression of membrane (m)IL-5Ralpha on bronchoalveolar lavage (BAL) eosinophils; 2) the responsiveness of these cells to exogenous IL-5; and 3) the presence of soluble (s)IL-5Ralpha in BAL fluid. To accomplish these goals, blood and BAL eosinophils were obtained from atopic subjects 48 h after segmental bronchoprovocation with Ag. There was a striking reduction in mIL-5Ralpha on airway eosinophils compared with circulating cells. Furthermore, sIL-5Ralpha concentrations were elevated in BAL fluid, but steady state levels of sIL-5Ralpha mRNA were not increased in BAL compared with blood eosinophils. Finally, BAL eosinophils were refractory to IL-5 for ex vivo degranulation, suggesting that the reduction in mIL-5Ralpha on BAL eosinophils may regulate IL-5-mediated eosinophil functions. Together, the loss of mIL-5Ralpha, the presence of sIL-5Ralpha, and the blunted functional response (degranulation) of eosinophils to IL-5 suggest that when eosinophils are recruited to the airway, regulation of their functions becomes IL-5 independent. These observations provide a potential explanation for the inability of anti-IL-5 therapy to suppress airway hyperresponsiveness to inhaled Ag, despite a reduction in eosinophil recruitment.

Authors+Show Affiliations

Allergy and Immunology, Department of Medicine, University of Wisconsin, Madison 53792, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12444154

Citation

Liu, Lin Ying, et al. "Decreased Expression of Membrane IL-5 Receptor Alpha On Human Eosinophils: I. Loss of Membrane IL-5 Receptor Alpha On Airway Eosinophils and Increased Soluble IL-5 Receptor Alpha in the Airway After Allergen Challenge." Journal of Immunology (Baltimore, Md. : 1950), vol. 169, no. 11, 2002, pp. 6452-8.
Liu LY, Sedgwick JB, Bates ME, et al. Decreased expression of membrane IL-5 receptor alpha on human eosinophils: I. Loss of membrane IL-5 receptor alpha on airway eosinophils and increased soluble IL-5 receptor alpha in the airway after allergen challenge. J Immunol. 2002;169(11):6452-8.
Liu, L. Y., Sedgwick, J. B., Bates, M. E., Vrtis, R. F., Gern, J. E., Kita, H., Jarjour, N. N., Busse, W. W., & Kelly, E. A. (2002). Decreased expression of membrane IL-5 receptor alpha on human eosinophils: I. Loss of membrane IL-5 receptor alpha on airway eosinophils and increased soluble IL-5 receptor alpha in the airway after allergen challenge. Journal of Immunology (Baltimore, Md. : 1950), 169(11), 6452-8.
Liu LY, et al. Decreased Expression of Membrane IL-5 Receptor Alpha On Human Eosinophils: I. Loss of Membrane IL-5 Receptor Alpha On Airway Eosinophils and Increased Soluble IL-5 Receptor Alpha in the Airway After Allergen Challenge. J Immunol. 2002 Dec 1;169(11):6452-8. PubMed PMID: 12444154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decreased expression of membrane IL-5 receptor alpha on human eosinophils: I. Loss of membrane IL-5 receptor alpha on airway eosinophils and increased soluble IL-5 receptor alpha in the airway after allergen challenge. AU - Liu,Lin Ying, AU - Sedgwick,Julie B, AU - Bates,Mary Ellen, AU - Vrtis,Rose F, AU - Gern,James E, AU - Kita,Hirohita, AU - Jarjour,Nizar N, AU - Busse,William W, AU - Kelly,Elizabeth A B, PY - 2002/11/22/pubmed PY - 2002/12/27/medline PY - 2002/11/22/entrez SP - 6452 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 169 IS - 11 N2 - IL-5 is a key cytokine for eosinophil maturation, recruitment, activation, and possibly the development of inflammation in asthma. High concentrations of IL-5 are present in the airway after Ag challenge, but the responsiveness of airway eosinophils to IL-5 is not well characterized. The objectives of this study were to establish, following airway Ag challenge: 1) the expression of membrane (m)IL-5Ralpha on bronchoalveolar lavage (BAL) eosinophils; 2) the responsiveness of these cells to exogenous IL-5; and 3) the presence of soluble (s)IL-5Ralpha in BAL fluid. To accomplish these goals, blood and BAL eosinophils were obtained from atopic subjects 48 h after segmental bronchoprovocation with Ag. There was a striking reduction in mIL-5Ralpha on airway eosinophils compared with circulating cells. Furthermore, sIL-5Ralpha concentrations were elevated in BAL fluid, but steady state levels of sIL-5Ralpha mRNA were not increased in BAL compared with blood eosinophils. Finally, BAL eosinophils were refractory to IL-5 for ex vivo degranulation, suggesting that the reduction in mIL-5Ralpha on BAL eosinophils may regulate IL-5-mediated eosinophil functions. Together, the loss of mIL-5Ralpha, the presence of sIL-5Ralpha, and the blunted functional response (degranulation) of eosinophils to IL-5 suggest that when eosinophils are recruited to the airway, regulation of their functions becomes IL-5 independent. These observations provide a potential explanation for the inability of anti-IL-5 therapy to suppress airway hyperresponsiveness to inhaled Ag, despite a reduction in eosinophil recruitment. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/12444154/Decreased_expression_of_membrane_IL_5_receptor_alpha_on_human_eosinophils:_I__Loss_of_membrane_IL_5_receptor_alpha_on_airway_eosinophils_and_increased_soluble_IL_5_receptor_alpha_in_the_airway_after_allergen_challenge_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=12444154 DB - PRIME DP - Unbound Medicine ER -