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Caenorhabditis elegans HUS-1 is a DNA damage checkpoint protein required for genome stability and EGL-1-mediated apoptosis.
Curr Biol. 2002 Nov 19; 12(22):1908-18.CB

Abstract

BACKGROUND

The inability to efficiently repair DNA damage or remove cells with severely damaged genomes has been linked to several human cancers. Studies in yeasts and mammals have identified several genes that are required for proper activation of cell cycle checkpoints following various types of DNA damage. However, in metazoans, DNA damage can induce apoptosis as well. How DNA damage activates the apoptotic machinery is not fully understood.

RESULTS

We demonstrate here that the Caenorhabditis elegans gene hus-1 is required for DNA damage-induced cell cycle arrest and apoptosis. Following DNA damage, HUS-1 relocalizes and forms distinct foci that overlap with chromatin. Relocalization does not require the novel checkpoint protein RAD-5; rather, relocalization appears more frequently in rad-5 mutants, suggesting that RAD-5 plays a role in repair. HUS-1 is required for genome stability, as demonstrated by increased frequency of spontaneous mutations, chromosome nondisjunction, and telomere shortening. Finally, we show that DNA damage increases expression of the proapoptotic gene egl-1, a response that requires hus-1 and the p53 homolog cep-1.

CONCLUSIONS

Our findings suggest that the RAD-5 checkpoint protein is not required for HUS-1 to relocalize following DNA damage. Furthermore, our studies reveal a new function of HUS-1 in the prevention of telomere shortening and mortalization of germ cells. DNA damage-induced germ cell death is abrogated in hus-1 mutants, in part, due to the inability of these mutants to activate egl-1 transcription in a cep-1/p53-dependent manner. Thus, HUS-1 is required for p53-dependent activation of a BH3 domain protein in C. elegans.

Authors+Show Affiliations

Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12445383

Citation

Hofmann, E Randal, et al. "Caenorhabditis Elegans HUS-1 Is a DNA Damage Checkpoint Protein Required for Genome Stability and EGL-1-mediated Apoptosis." Current Biology : CB, vol. 12, no. 22, 2002, pp. 1908-18.
Hofmann ER, Milstein S, Boulton SJ, et al. Caenorhabditis elegans HUS-1 is a DNA damage checkpoint protein required for genome stability and EGL-1-mediated apoptosis. Curr Biol. 2002;12(22):1908-18.
Hofmann, E. R., Milstein, S., Boulton, S. J., Ye, M., Hofmann, J. J., Stergiou, L., Gartner, A., Vidal, M., & Hengartner, M. O. (2002). Caenorhabditis elegans HUS-1 is a DNA damage checkpoint protein required for genome stability and EGL-1-mediated apoptosis. Current Biology : CB, 12(22), 1908-18.
Hofmann ER, et al. Caenorhabditis Elegans HUS-1 Is a DNA Damage Checkpoint Protein Required for Genome Stability and EGL-1-mediated Apoptosis. Curr Biol. 2002 Nov 19;12(22):1908-18. PubMed PMID: 12445383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caenorhabditis elegans HUS-1 is a DNA damage checkpoint protein required for genome stability and EGL-1-mediated apoptosis. AU - Hofmann,E Randal, AU - Milstein,Stuart, AU - Boulton,Simon J, AU - Ye,Mianjia, AU - Hofmann,Jen J, AU - Stergiou,Lilli, AU - Gartner,Anton, AU - Vidal,Marc, AU - Hengartner,Michael O, PY - 2002/11/26/pubmed PY - 2003/6/17/medline PY - 2002/11/26/entrez SP - 1908 EP - 18 JF - Current biology : CB JO - Curr Biol VL - 12 IS - 22 N2 - BACKGROUND: The inability to efficiently repair DNA damage or remove cells with severely damaged genomes has been linked to several human cancers. Studies in yeasts and mammals have identified several genes that are required for proper activation of cell cycle checkpoints following various types of DNA damage. However, in metazoans, DNA damage can induce apoptosis as well. How DNA damage activates the apoptotic machinery is not fully understood. RESULTS: We demonstrate here that the Caenorhabditis elegans gene hus-1 is required for DNA damage-induced cell cycle arrest and apoptosis. Following DNA damage, HUS-1 relocalizes and forms distinct foci that overlap with chromatin. Relocalization does not require the novel checkpoint protein RAD-5; rather, relocalization appears more frequently in rad-5 mutants, suggesting that RAD-5 plays a role in repair. HUS-1 is required for genome stability, as demonstrated by increased frequency of spontaneous mutations, chromosome nondisjunction, and telomere shortening. Finally, we show that DNA damage increases expression of the proapoptotic gene egl-1, a response that requires hus-1 and the p53 homolog cep-1. CONCLUSIONS: Our findings suggest that the RAD-5 checkpoint protein is not required for HUS-1 to relocalize following DNA damage. Furthermore, our studies reveal a new function of HUS-1 in the prevention of telomere shortening and mortalization of germ cells. DNA damage-induced germ cell death is abrogated in hus-1 mutants, in part, due to the inability of these mutants to activate egl-1 transcription in a cep-1/p53-dependent manner. Thus, HUS-1 is required for p53-dependent activation of a BH3 domain protein in C. elegans. SN - 0960-9822 UR - https://www.unboundmedicine.com/medline/citation/12445383/Caenorhabditis_elegans_HUS_1_is_a_DNA_damage_checkpoint_protein_required_for_genome_stability_and_EGL_1_mediated_apoptosis_ DB - PRIME DP - Unbound Medicine ER -