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Effects of selenium overexposure on glutathione peroxidase and thioredoxin reductase gene expressions and activities.
Biol Trace Elem Res 2002; 89(2):165-75BT

Abstract

Selenium (Se) is an essential trace element in animals and human, however, excess Se intake can result in adverse health effects. Se supplementation increased glutathione peroxidase (GSH-Px) and thioredoxin reductase (TR) expressions in Se-deficient rats. However, little information is available on the relationship between Se overexposure on GSH-Px and TR mRNA levels and activities. In this study, the effects of Se overexposure on GSH-Px and TR mRNA levels and activities were investigated by reverse transcription-polymerase chain reaction (RT-PCR) and activity assay. Experimental groups of male Wistar rats were injected intraperitoneally (i.p.) with sodium selenite at doses of 20, 40, and 80 microg Se/kg/d for 15 d, respectively. Se levels were elevated dose-dependently in rat liver, kidney, and testis tissues. GSH-Px mRNA levels and activities in the liver and testis for rats injected with 20 microg Se/kg/d were increased significantly as compared with those in the control group. However, intraperitoneal injection of 40 and 80 microg Se/kg/d dramatically decreased GSH-Px mRNA expression and activity in liver and testis. The changes of TR mRNA level and activity in the liver and kidney were similar to those of GSH-Px in the liver and testis when supplemented with 40 and 80 microg Se/kg/d. There were no significant effects of Se status on kidney GSH-Px and testis TR expressions. The results suggested that Se overexposure had an adverse effect on GSH-Px and TR mRNA levels and activities, and there could be tissue differences in the regulation of selenoprotein levels.

Authors+Show Affiliations

Department of Chemistry, Huazhong University of Science and Technology, Wuhan, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12449240

Citation

Gan, Lu, et al. "Effects of Selenium Overexposure On Glutathione Peroxidase and Thioredoxin Reductase Gene Expressions and Activities." Biological Trace Element Research, vol. 89, no. 2, 2002, pp. 165-75.
Gan L, Liu Q, Xu HB, et al. Effects of selenium overexposure on glutathione peroxidase and thioredoxin reductase gene expressions and activities. Biol Trace Elem Res. 2002;89(2):165-75.
Gan, L., Liu, Q., Xu, H. B., Zhu, Y. S., & Yang, X. L. (2002). Effects of selenium overexposure on glutathione peroxidase and thioredoxin reductase gene expressions and activities. Biological Trace Element Research, 89(2), pp. 165-75.
Gan L, et al. Effects of Selenium Overexposure On Glutathione Peroxidase and Thioredoxin Reductase Gene Expressions and Activities. Biol Trace Elem Res. 2002;89(2):165-75. PubMed PMID: 12449240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of selenium overexposure on glutathione peroxidase and thioredoxin reductase gene expressions and activities. AU - Gan,Lu, AU - Liu,Qiong, AU - Xu,Hui-Bi, AU - Zhu,Yu-Shan, AU - Yang,Xiang-Liang, PY - 2002/11/27/pubmed PY - 2003/12/3/medline PY - 2002/11/27/entrez SP - 165 EP - 75 JF - Biological trace element research JO - Biol Trace Elem Res VL - 89 IS - 2 N2 - Selenium (Se) is an essential trace element in animals and human, however, excess Se intake can result in adverse health effects. Se supplementation increased glutathione peroxidase (GSH-Px) and thioredoxin reductase (TR) expressions in Se-deficient rats. However, little information is available on the relationship between Se overexposure on GSH-Px and TR mRNA levels and activities. In this study, the effects of Se overexposure on GSH-Px and TR mRNA levels and activities were investigated by reverse transcription-polymerase chain reaction (RT-PCR) and activity assay. Experimental groups of male Wistar rats were injected intraperitoneally (i.p.) with sodium selenite at doses of 20, 40, and 80 microg Se/kg/d for 15 d, respectively. Se levels were elevated dose-dependently in rat liver, kidney, and testis tissues. GSH-Px mRNA levels and activities in the liver and testis for rats injected with 20 microg Se/kg/d were increased significantly as compared with those in the control group. However, intraperitoneal injection of 40 and 80 microg Se/kg/d dramatically decreased GSH-Px mRNA expression and activity in liver and testis. The changes of TR mRNA level and activity in the liver and kidney were similar to those of GSH-Px in the liver and testis when supplemented with 40 and 80 microg Se/kg/d. There were no significant effects of Se status on kidney GSH-Px and testis TR expressions. The results suggested that Se overexposure had an adverse effect on GSH-Px and TR mRNA levels and activities, and there could be tissue differences in the regulation of selenoprotein levels. SN - 0163-4984 UR - https://www.unboundmedicine.com/medline/citation/12449240/Effects_of_selenium_overexposure_on_glutathione_peroxidase_and_thioredoxin_reductase_gene_expressions_and_activities_ L2 - https://dx.doi.org/10.1385/BTER:89:2:165 DB - PRIME DP - Unbound Medicine ER -