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Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum.
Vet Pathol. 2002 Nov; 39(6):712-20.VP

Abstract

Salmonella enterica serovar Typhimurium is an important intestinal pathogen in swine. This study was performed to document the early cellular invasion of Salmonella serovar Typhimurium in swine ileum. Ileal gut-loops were surgically prepared in ten 4- to 5-week-old mixed-breed pigs and inoculated for 0-60 minutes. Loops were harvested and prepared for both scanning and transmission electron microscopy (SEM and TEM, respectively). Preferential bacterial adherence to microfold cells (M cells) was seen within 5 minutes, and by 10 minutes bacterial invasion of the apical membrane was seen in M cells, goblet cells, and enterocytes. This multicellular invasion was observed throughout the course of infection. In addition, SEM revealed a specific affinity of Salmonella serovar Typhimurium to sites of cell extrusion. Using TEM, bacteria in these areas were focused in the crevices formed by the extruding cell and the adjacent cells and in the cytoplasm immediately beneath the extruding cell. Our results suggest that early cellular invasion by Salmonella serovar Typhimurium is nonspecific and rapid in swine. Furthermore, the combination of SEM and TEM data suggests that Salmonella serovar Typhimurium may use sites of cell extrusion as an additional mechanism for early invasion.

Authors+Show Affiliations

Department of Veterinary Microbiology, Iowa State University, Ames, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12450202

Citation

Meyerholz, D K., et al. "Early Epithelial Invasion By Salmonella Enterica Serovar Typhimurium DT104 in the Swine Ileum." Veterinary Pathology, vol. 39, no. 6, 2002, pp. 712-20.
Meyerholz DK, Stabel TJ, Ackermann MR, et al. Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum. Vet Pathol. 2002;39(6):712-20.
Meyerholz, D. K., Stabel, T. J., Ackermann, M. R., Carlson, S. A., Jones, B. D., & Pohlenz, J. (2002). Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum. Veterinary Pathology, 39(6), 712-20.
Meyerholz DK, et al. Early Epithelial Invasion By Salmonella Enterica Serovar Typhimurium DT104 in the Swine Ileum. Vet Pathol. 2002;39(6):712-20. PubMed PMID: 12450202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum. AU - Meyerholz,D K, AU - Stabel,T J, AU - Ackermann,M R, AU - Carlson,S A, AU - Jones,B D, AU - Pohlenz,J, PY - 2002/11/27/pubmed PY - 2003/2/25/medline PY - 2002/11/27/entrez SP - 712 EP - 20 JF - Veterinary pathology JO - Vet Pathol VL - 39 IS - 6 N2 - Salmonella enterica serovar Typhimurium is an important intestinal pathogen in swine. This study was performed to document the early cellular invasion of Salmonella serovar Typhimurium in swine ileum. Ileal gut-loops were surgically prepared in ten 4- to 5-week-old mixed-breed pigs and inoculated for 0-60 minutes. Loops were harvested and prepared for both scanning and transmission electron microscopy (SEM and TEM, respectively). Preferential bacterial adherence to microfold cells (M cells) was seen within 5 minutes, and by 10 minutes bacterial invasion of the apical membrane was seen in M cells, goblet cells, and enterocytes. This multicellular invasion was observed throughout the course of infection. In addition, SEM revealed a specific affinity of Salmonella serovar Typhimurium to sites of cell extrusion. Using TEM, bacteria in these areas were focused in the crevices formed by the extruding cell and the adjacent cells and in the cytoplasm immediately beneath the extruding cell. Our results suggest that early cellular invasion by Salmonella serovar Typhimurium is nonspecific and rapid in swine. Furthermore, the combination of SEM and TEM data suggests that Salmonella serovar Typhimurium may use sites of cell extrusion as an additional mechanism for early invasion. SN - 0300-9858 UR - https://www.unboundmedicine.com/medline/citation/12450202/Early_epithelial_invasion_by_Salmonella_enterica_serovar_Typhimurium_DT104_in_the_swine_ileum_ L2 - https://journals.sagepub.com/doi/10.1354/vp.39-6-712?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -