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Developmental expression of skeletal muscle heparan sulfate proteoglycans in turkeys with different growth rates.
Poult Sci. 2002 Nov; 81(11):1621-8.PS

Abstract

Heparan sulfate proteoglycans (HSPG) are a group of extracellular matrix molecules that link skeletal muscle cells to their extrinsic environment. To investigate if HSPG expression is affected by muscle growth and gender, a turkey line (F) selected for increased 16-wk BW and its unselected random-bred control line, RBC2, were used in the present study. Heparan sulfate (HS) and HSPG levels were measured in embryonic and posthatch pectoralis major muscle. HS levels plateaued at embryonic day (ED) 16 in both lines. A significant decrease of HS occurred at ED 18 in F males and females, and at ED 20 and 22 in the RBC2 males and females, respectively. Embryonic HSPG levels peaked at ED 18, and were significantly higher from ED 14 through 18 in F males and females compared with those of the RBC2 line. Male pectoralis major muscle had more HSPG at early embryonic stages than female muscle in both lines. During 1 to 16 wk posthatch, F male and female pectoralis major muscle contained more HSPG than the RBC2 samples, and HSPG levels in F males were higher than those of the females. Myogenic satellite cells derived from F and RBC2 male and female pectoralis major muscle were cultured to measure HSPG expression during proliferation and differentiation. No significant difference in HSPG level was found between the RBC2 and F line cells. However, in both lines, male-derived satellite cells had more HSPG than the female cells during proliferation and differentiation. These data show that HS and HSPG expression are affected by muscle growth properties and sex.

Authors+Show Affiliations

Liu X
Department of Animal Sciences, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, Ohio, USA.
Nestor KE
No affiliation info available
McFarland DC
No affiliation info available
Velleman SG
No affiliation info available

MeSH

Age FactorsAnimalsBody WeightBreedingCell DifferentiationCell DivisionCells, CulturedFemaleHeparan Sulfate ProteoglycansHeparitin SulfateMaleMuscle DevelopmentPectoralis MusclesSatellite Cells, Skeletal MuscleSelection, GeneticSex FactorsTurkeys

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12455586

Citation

Liu, X, et al. "Developmental Expression of Skeletal Muscle Heparan Sulfate Proteoglycans in Turkeys With Different Growth Rates." Poultry Science, vol. 81, no. 11, 2002, pp. 1621-8.
Liu X, Nestor KE, McFarland DC, et al. Developmental expression of skeletal muscle heparan sulfate proteoglycans in turkeys with different growth rates. Poult Sci. 2002;81(11):1621-8.
Liu, X., Nestor, K. E., McFarland, D. C., & Velleman, S. G. (2002). Developmental expression of skeletal muscle heparan sulfate proteoglycans in turkeys with different growth rates. Poultry Science, 81(11), 1621-8.
Liu X, et al. Developmental Expression of Skeletal Muscle Heparan Sulfate Proteoglycans in Turkeys With Different Growth Rates. Poult Sci. 2002;81(11):1621-8. PubMed PMID: 12455586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental expression of skeletal muscle heparan sulfate proteoglycans in turkeys with different growth rates. AU - Liu,X, AU - Nestor,K E, AU - McFarland,D C, AU - Velleman,S G, PY - 2002/11/29/pubmed PY - 2003/3/21/medline PY - 2002/11/29/entrez SP - 1621 EP - 8 JF - Poultry science JO - Poult Sci VL - 81 IS - 11 N2 - Heparan sulfate proteoglycans (HSPG) are a group of extracellular matrix molecules that link skeletal muscle cells to their extrinsic environment. To investigate if HSPG expression is affected by muscle growth and gender, a turkey line (F) selected for increased 16-wk BW and its unselected random-bred control line, RBC2, were used in the present study. Heparan sulfate (HS) and HSPG levels were measured in embryonic and posthatch pectoralis major muscle. HS levels plateaued at embryonic day (ED) 16 in both lines. A significant decrease of HS occurred at ED 18 in F males and females, and at ED 20 and 22 in the RBC2 males and females, respectively. Embryonic HSPG levels peaked at ED 18, and were significantly higher from ED 14 through 18 in F males and females compared with those of the RBC2 line. Male pectoralis major muscle had more HSPG at early embryonic stages than female muscle in both lines. During 1 to 16 wk posthatch, F male and female pectoralis major muscle contained more HSPG than the RBC2 samples, and HSPG levels in F males were higher than those of the females. Myogenic satellite cells derived from F and RBC2 male and female pectoralis major muscle were cultured to measure HSPG expression during proliferation and differentiation. No significant difference in HSPG level was found between the RBC2 and F line cells. However, in both lines, male-derived satellite cells had more HSPG than the female cells during proliferation and differentiation. These data show that HS and HSPG expression are affected by muscle growth properties and sex. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/12455586/Developmental_expression_of_skeletal_muscle_heparan_sulfate_proteoglycans_in_turkeys_with_different_growth_rates_ DB - PRIME DP - Unbound Medicine ER -