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Distribution and possible function of an adrenomedullin-like peptide in the developing chick limb bud.
Int J Dev Biol. 2002; 46(7):957-61.IJ

Abstract

Adrenomedullin (AM) is a multifunctional peptide that exhibits discrete domains of expression during mouse embryogenesis consistent with a role in regulating growth and differentiation during morphogenesis. Here we report that AM immunoreactivity is present at high levels throughout the apical ectodermal ridge (AER) of the chick limb bud as the AER is directing the outgrowth and patterning of underlying limb mesoderm. Immunostaining is particularly strong along the surfaces of the contiguous cells of the AER. AM immunoreactivity attenuates as the AER regresses and is absent from the distal apical ectoderm of stage 20 limbless mutant limb buds which fail to develop an AER. To explore the possible role of AM in AER activity, we examined the effect of exogenous AM and an AM inhibitor on the in vitro morphogenesis of limb mesoderm, cultured in the presence and absence of the AER. Although exogenous AM cannot substitute for the AER in promoting outgrowth of limb mesoderm in vitro, a specific AM antagonist, AM(22-52), impairs the outgrowth and proliferation of limb mesoderm cultured in the presence of the AER. This is consistent with the possibility that inhibition of endogenous AM activity in the AER impairs the ability of the AER to promote limb morphogenesis. Taken together, these studies suggest that an AM-like molecule may function in an autocrine fashion to regulate some aspect of AER activity.

Authors+Show Affiliations

University of Connecticut Health Center, Farmington, Connecticut 06030, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12455634

Citation

Seghatoleslami, M Reza, et al. "Distribution and Possible Function of an Adrenomedullin-like Peptide in the Developing Chick Limb Bud." The International Journal of Developmental Biology, vol. 46, no. 7, 2002, pp. 957-61.
Seghatoleslami MR, Martínez A, Cuttitta F, et al. Distribution and possible function of an adrenomedullin-like peptide in the developing chick limb bud. Int J Dev Biol. 2002;46(7):957-61.
Seghatoleslami, M. R., Martínez, A., Cuttitta, F., & Kosher, R. A. (2002). Distribution and possible function of an adrenomedullin-like peptide in the developing chick limb bud. The International Journal of Developmental Biology, 46(7), 957-61.
Seghatoleslami MR, et al. Distribution and Possible Function of an Adrenomedullin-like Peptide in the Developing Chick Limb Bud. Int J Dev Biol. 2002;46(7):957-61. PubMed PMID: 12455634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution and possible function of an adrenomedullin-like peptide in the developing chick limb bud. AU - Seghatoleslami,M Reza, AU - Martínez,Alfredo, AU - Cuttitta,Frank, AU - Kosher,Robert A, PY - 2002/11/29/pubmed PY - 2003/6/19/medline PY - 2002/11/29/entrez SP - 957 EP - 61 JF - The International journal of developmental biology JO - Int J Dev Biol VL - 46 IS - 7 N2 - Adrenomedullin (AM) is a multifunctional peptide that exhibits discrete domains of expression during mouse embryogenesis consistent with a role in regulating growth and differentiation during morphogenesis. Here we report that AM immunoreactivity is present at high levels throughout the apical ectodermal ridge (AER) of the chick limb bud as the AER is directing the outgrowth and patterning of underlying limb mesoderm. Immunostaining is particularly strong along the surfaces of the contiguous cells of the AER. AM immunoreactivity attenuates as the AER regresses and is absent from the distal apical ectoderm of stage 20 limbless mutant limb buds which fail to develop an AER. To explore the possible role of AM in AER activity, we examined the effect of exogenous AM and an AM inhibitor on the in vitro morphogenesis of limb mesoderm, cultured in the presence and absence of the AER. Although exogenous AM cannot substitute for the AER in promoting outgrowth of limb mesoderm in vitro, a specific AM antagonist, AM(22-52), impairs the outgrowth and proliferation of limb mesoderm cultured in the presence of the AER. This is consistent with the possibility that inhibition of endogenous AM activity in the AER impairs the ability of the AER to promote limb morphogenesis. Taken together, these studies suggest that an AM-like molecule may function in an autocrine fashion to regulate some aspect of AER activity. SN - 0214-6282 UR - https://www.unboundmedicine.com/medline/citation/12455634/Distribution_and_possible_function_of_an_adrenomedullin_like_peptide_in_the_developing_chick_limb_bud_ L2 - http://www.intjdevbiol.com/paper.php?doi=12455634 DB - PRIME DP - Unbound Medicine ER -