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Analysis of toxin profiles in three different fish species causing ciguatera fish poisoning in Guadeloupe, French West Indies.
Food Addit Contam. 2002 Nov; 19(11):1034-42.FA

Abstract

A grey snapper (Lutjanus griseus), a grouper (Serranidae) and a black jack (Caranx lugubris) were implicated in three different ciguatera poisonings in Guadeloupe, French West Indies. A mouse bioassay indicated toxicity for each specimens: 0.5-1, > or = 1 and > 1 MUg g(-1), respectively. After purification by gel filtration chromatography, the samples were analysed by high-performance liquid chromatography coupled to mass spectrometry (LC-MS). The toxin profiles differ from one fish to another. C-CTX-1 was detected at 0.24, 0.90 and 13.8 ng g(-1) flesh in the snapper, grouper and jack, respectively. It contributed only to part of the whole toxicity determined by the mouse bioassay. Other toxins identified were C-CTX-2 (a C-CTX-1 epimer), three additional isomers of C-CTX-1 or-2, and five ciguatoxin congeners (C-CTX-1127, C-CTX-1143 and its isomer C-CTX-1143a, and C-CTX-1157 and its isomer C-CTX-1157b). Putative hydroxy-polyether-like compounds were also detected in the flesh of the grouper with [M+ + H]+ ions at m/z 851.51, 857.50, 875.51, 875.49 and 895.54 Da. Some of these compounds have the same mass range as some known dinoflagellate toxins. In conclusion, this study confirms the usefulness of LC-MS analysis to determine the ciguatoxins levels and the toxin profile in fish flesh hazardous to humans.

Authors+Show Affiliations

Laboratoire de Microbiologie Alimentaire USC INRA, Universite de Caen, Esplanade de la Paix F-14032 Caen cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12456274

Citation

Pottier, I, et al. "Analysis of Toxin Profiles in Three Different Fish Species Causing Ciguatera Fish Poisoning in Guadeloupe, French West Indies." Food Additives and Contaminants, vol. 19, no. 11, 2002, pp. 1034-42.
Pottier I, Vernoux JP, Jones A, et al. Analysis of toxin profiles in three different fish species causing ciguatera fish poisoning in Guadeloupe, French West Indies. Food Addit Contam. 2002;19(11):1034-42.
Pottier, I., Vernoux, J. P., Jones, A., & Lewis, R. J. (2002). Analysis of toxin profiles in three different fish species causing ciguatera fish poisoning in Guadeloupe, French West Indies. Food Additives and Contaminants, 19(11), 1034-42.
Pottier I, et al. Analysis of Toxin Profiles in Three Different Fish Species Causing Ciguatera Fish Poisoning in Guadeloupe, French West Indies. Food Addit Contam. 2002;19(11):1034-42. PubMed PMID: 12456274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of toxin profiles in three different fish species causing ciguatera fish poisoning in Guadeloupe, French West Indies. AU - Pottier,I, AU - Vernoux,J P, AU - Jones,A, AU - Lewis,R J, PY - 2002/11/29/pubmed PY - 2002/12/18/medline PY - 2002/11/29/entrez SP - 1034 EP - 42 JF - Food additives and contaminants JO - Food Addit Contam VL - 19 IS - 11 N2 - A grey snapper (Lutjanus griseus), a grouper (Serranidae) and a black jack (Caranx lugubris) were implicated in three different ciguatera poisonings in Guadeloupe, French West Indies. A mouse bioassay indicated toxicity for each specimens: 0.5-1, > or = 1 and > 1 MUg g(-1), respectively. After purification by gel filtration chromatography, the samples were analysed by high-performance liquid chromatography coupled to mass spectrometry (LC-MS). The toxin profiles differ from one fish to another. C-CTX-1 was detected at 0.24, 0.90 and 13.8 ng g(-1) flesh in the snapper, grouper and jack, respectively. It contributed only to part of the whole toxicity determined by the mouse bioassay. Other toxins identified were C-CTX-2 (a C-CTX-1 epimer), three additional isomers of C-CTX-1 or-2, and five ciguatoxin congeners (C-CTX-1127, C-CTX-1143 and its isomer C-CTX-1143a, and C-CTX-1157 and its isomer C-CTX-1157b). Putative hydroxy-polyether-like compounds were also detected in the flesh of the grouper with [M+ + H]+ ions at m/z 851.51, 857.50, 875.51, 875.49 and 895.54 Da. Some of these compounds have the same mass range as some known dinoflagellate toxins. In conclusion, this study confirms the usefulness of LC-MS analysis to determine the ciguatoxins levels and the toxin profile in fish flesh hazardous to humans. SN - 0265-203X UR - https://www.unboundmedicine.com/medline/citation/12456274/Analysis_of_toxin_profiles_in_three_different_fish_species_causing_ciguatera_fish_poisoning_in_Guadeloupe_French_West_Indies_ L2 - http://www.diseaseinfosearch.org/result/1638 DB - PRIME DP - Unbound Medicine ER -