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Expression of recombinant proteins of Orientia tsutsugamushi and their applications in the serodiagnosis of scrub typhus.
Diagn Microbiol Infect Dis. 2002 Oct; 44(2):137-42.DM

Abstract

In this study, recombinant proteins that encompassed the AD I-AD III regions of 56 kDa immunodominant gene of 2 Orientia tsutsugamushi (OT) serotypes; Gilliam and TA763 were expressed in Escherichia coli. Both recombinant proteins exhibited serologic cross-reactivity with the rabbit antisera against various OT serotypes, as evaluated by enzyme-linked immunosorbent assay (ELISA), but not against other rickettsial species, including Rickettsia typhi, R. prowazekii and TT118 SFG rickettsiae. The feasibility of using the recombinant proteins as a diagnostic reagent was further evaluated by ELISA using sera from blood donors and scrub typhus patients. The results suggested a higher affinity of the antihuman IgM than IgG with both recombinant proteins. The IgM ELISA findings were agreeable with the results of indirect immunoperoxidase (IIP) assay especially with sera of high antibody (1:1600). However, more than one antigen are probably needed for development of an effective assay for serodiagnosis of scrub typhus in endemic areas.

Authors+Show Affiliations

Tay ST
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Rohani MY
No affiliation info available
Ho TM
No affiliation info available
Devi S
No affiliation info available

MeSH

AnimalsAntibodies, BacterialBase SequenceCross ReactionsDNA, BacterialEnzyme-Linked Immunosorbent AssayHumansImmunoenzyme TechniquesMolecular Sequence DataOrientia tsutsugamushiPolymerase Chain ReactionRabbitsRecombinant ProteinsScrub TyphusSensitivity and SpecificitySerologic TestsSpecies Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12458119

Citation

Tay, S T., et al. "Expression of Recombinant Proteins of Orientia Tsutsugamushi and Their Applications in the Serodiagnosis of Scrub Typhus." Diagnostic Microbiology and Infectious Disease, vol. 44, no. 2, 2002, pp. 137-42.
Tay ST, Rohani MY, Ho TM, et al. Expression of recombinant proteins of Orientia tsutsugamushi and their applications in the serodiagnosis of scrub typhus. Diagn Microbiol Infect Dis. 2002;44(2):137-42.
Tay, S. T., Rohani, M. Y., Ho, T. M., & Devi, S. (2002). Expression of recombinant proteins of Orientia tsutsugamushi and their applications in the serodiagnosis of scrub typhus. Diagnostic Microbiology and Infectious Disease, 44(2), 137-42.
Tay ST, et al. Expression of Recombinant Proteins of Orientia Tsutsugamushi and Their Applications in the Serodiagnosis of Scrub Typhus. Diagn Microbiol Infect Dis. 2002;44(2):137-42. PubMed PMID: 12458119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of recombinant proteins of Orientia tsutsugamushi and their applications in the serodiagnosis of scrub typhus. AU - Tay,S T, AU - Rohani,M Y, AU - Ho,T M, AU - Devi,S, PY - 2002/11/30/pubmed PY - 2003/1/24/medline PY - 2002/11/30/entrez SP - 137 EP - 42 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 44 IS - 2 N2 - In this study, recombinant proteins that encompassed the AD I-AD III regions of 56 kDa immunodominant gene of 2 Orientia tsutsugamushi (OT) serotypes; Gilliam and TA763 were expressed in Escherichia coli. Both recombinant proteins exhibited serologic cross-reactivity with the rabbit antisera against various OT serotypes, as evaluated by enzyme-linked immunosorbent assay (ELISA), but not against other rickettsial species, including Rickettsia typhi, R. prowazekii and TT118 SFG rickettsiae. The feasibility of using the recombinant proteins as a diagnostic reagent was further evaluated by ELISA using sera from blood donors and scrub typhus patients. The results suggested a higher affinity of the antihuman IgM than IgG with both recombinant proteins. The IgM ELISA findings were agreeable with the results of indirect immunoperoxidase (IIP) assay especially with sera of high antibody (1:1600). However, more than one antigen are probably needed for development of an effective assay for serodiagnosis of scrub typhus in endemic areas. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/12458119/Expression_of_recombinant_proteins_of_Orientia_tsutsugamushi_and_their_applications_in_the_serodiagnosis_of_scrub_typhus_ DB - PRIME DP - Unbound Medicine ER -