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Procyanidin B-3, isolated from barley and identified as a hair-growth stimulant, has the potential to counteract inhibitory regulation by TGF-beta1.
Exp Dermatol. 2002 Dec; 11(6):532-41.ED

Abstract

With the aim of identifying natural products, which possess hair-growing activity, we examined more than 1000 plant extracts with respect to their growth-promoting effects on hair epithelial cells. We discovered intensive growth-promoting activity, about 140% relative to controls, in barley extract. Our strategy for identifying active compounds in barley extract involved subjecting it to column chromatography using HP-20 resin columns, an LH-20 resin column, and preparative high-performance liquid chromatography (HPLC) using an ODS column. The 60% (v/v) aqueous methanol eluted fraction from the HP-20 column and the 75% (v/v) aqueous methanol eluted fraction from the subsequent LH-20 column showed high hair-growing activity in vivo. We isolated two major substances from the LH-20 active fraction using preparative HPLC. By means of mass spectrometry, 1H-NMR, and 13C-NMR analyses, one substance was revealed to be procyanidin B-3 and the other substance was identified as (+)-catechin. Purified procyanidin B-3 showed high hair-growing activity in the form of in vitro hair epithelial cell growth-promoting activity and in vivo anagen-inducing activity; however (+)-catechin showed no hair-growing activity. For the purpose of examining the hair-growing mechanisms of procyanidin B-3, we examined its relationship to the TGF-beta signal pathway, which is known to be a regulator of catagen induction. Addition of TGF-beta1 to hair epithelial cell cultures dose-dependently decreased the cell growth, and addition of procyanidin B-3 to the culture neutralized the growth-inhibiting effect of TGF-beta1. From these results, it is concluded that procyanidin B-3 can directly promote hair epithelial cell growth in vitro, has the potential to counteract the growth-inhibiting effect caused by TGF-beta1 in vitro, and has potential to stimulate anagen induction in vivo.

Authors+Show Affiliations

Tsukuba Research Laboratories, Kyowa Hakko Kogyo Co, Tsukuba, Ibaraki, Japan. ayako.kamimura@kyowa.co.jpNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12473061

Citation

Kamimura, Ayako, and Tomoya Takahashi. "Procyanidin B-3, Isolated From Barley and Identified as a Hair-growth Stimulant, Has the Potential to Counteract Inhibitory Regulation By TGF-beta1." Experimental Dermatology, vol. 11, no. 6, 2002, pp. 532-41.
Kamimura A, Takahashi T. Procyanidin B-3, isolated from barley and identified as a hair-growth stimulant, has the potential to counteract inhibitory regulation by TGF-beta1. Exp Dermatol. 2002;11(6):532-41.
Kamimura, A., & Takahashi, T. (2002). Procyanidin B-3, isolated from barley and identified as a hair-growth stimulant, has the potential to counteract inhibitory regulation by TGF-beta1. Experimental Dermatology, 11(6), 532-41.
Kamimura A, Takahashi T. Procyanidin B-3, Isolated From Barley and Identified as a Hair-growth Stimulant, Has the Potential to Counteract Inhibitory Regulation By TGF-beta1. Exp Dermatol. 2002;11(6):532-41. PubMed PMID: 12473061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Procyanidin B-3, isolated from barley and identified as a hair-growth stimulant, has the potential to counteract inhibitory regulation by TGF-beta1. AU - Kamimura,Ayako, AU - Takahashi,Tomoya, PY - 2002/12/11/pubmed PY - 2003/7/19/medline PY - 2002/12/11/entrez SP - 532 EP - 41 JF - Experimental dermatology JO - Exp Dermatol VL - 11 IS - 6 N2 - With the aim of identifying natural products, which possess hair-growing activity, we examined more than 1000 plant extracts with respect to their growth-promoting effects on hair epithelial cells. We discovered intensive growth-promoting activity, about 140% relative to controls, in barley extract. Our strategy for identifying active compounds in barley extract involved subjecting it to column chromatography using HP-20 resin columns, an LH-20 resin column, and preparative high-performance liquid chromatography (HPLC) using an ODS column. The 60% (v/v) aqueous methanol eluted fraction from the HP-20 column and the 75% (v/v) aqueous methanol eluted fraction from the subsequent LH-20 column showed high hair-growing activity in vivo. We isolated two major substances from the LH-20 active fraction using preparative HPLC. By means of mass spectrometry, 1H-NMR, and 13C-NMR analyses, one substance was revealed to be procyanidin B-3 and the other substance was identified as (+)-catechin. Purified procyanidin B-3 showed high hair-growing activity in the form of in vitro hair epithelial cell growth-promoting activity and in vivo anagen-inducing activity; however (+)-catechin showed no hair-growing activity. For the purpose of examining the hair-growing mechanisms of procyanidin B-3, we examined its relationship to the TGF-beta signal pathway, which is known to be a regulator of catagen induction. Addition of TGF-beta1 to hair epithelial cell cultures dose-dependently decreased the cell growth, and addition of procyanidin B-3 to the culture neutralized the growth-inhibiting effect of TGF-beta1. From these results, it is concluded that procyanidin B-3 can directly promote hair epithelial cell growth in vitro, has the potential to counteract the growth-inhibiting effect caused by TGF-beta1 in vitro, and has potential to stimulate anagen induction in vivo. SN - 0906-6705 UR - https://www.unboundmedicine.com/medline/citation/12473061/Procyanidin_B_3_isolated_from_barley_and_identified_as_a_hair_growth_stimulant_has_the_potential_to_counteract_inhibitory_regulation_by_TGF_beta1_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0906-6705&date=2002&volume=11&issue=6&spage=532 DB - PRIME DP - Unbound Medicine ER -