TY - JOUR T1 - Design and synthesis of a hydrophilic fluorescent derivatization reagent for carboxylic acids, 4-N-(4-N-aminoethyl)piperazino-7-nitro-2,1,3-benzoxadiazole (NBD-PZ-NH2), and its application to capillary electrophoresis with laser-induced fluorescence detection. AU - Santa,Tomofumi, AU - Matsumura,Daisuke, AU - Huang,ChengZhi, AU - Kitada,Chieko, AU - Imai,Kazuhiro, PY - 2002/12/11/pubmed PY - 2003/4/18/medline PY - 2002/12/11/entrez SP - 523 EP - 8 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 16 IS - 8 N2 - A hydrophilic fluorescent derivatization reagent for fatty acids, 4-N-(4-N-aminoethyl)piperazino-7-nitro-2,1,3-benzoxadiazole (NBD-PZ-NH(2)), was designed and synthesized. NBD-PZ-NH(2) possesses not only a fluorophore and a reacting group but also a positive charge group and, thus, was hydrophilic and suitable for application to capillary electrophoresis. NBD-PZ-NH(2) reacted with fatty acids in the presence of triphenylphosphine (TPP) and 2,2'-dipyridyl disulfide (DPDS) at room temperature within 10 min. The derivatives were strongly fluoresced and were positively charged at pH below 3. The derivatives of C4-C20 fatty acids were separated within 10 min in 50% acetonitrile in water containing 30 mM ammonium acetate and 1.0 M acetic acid by capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. The detection limits attained were 6.5 nM (signal-to-noise ratio of 3). It is proposed that NBD-PZ-NH(2) is a prominent derivatization reagent for fatty acids which is suitable for CE-LIF application. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/12474216/Design_and_synthesis_of_a_hydrophilic_fluorescent_derivatization_reagent_for_carboxylic_acids_4_N__4_N_aminoethyl_piperazino_7_nitro_213_benzoxadiazole__NBD_PZ_NH2__and_its_application_to_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -