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Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand.
Am J Trop Med Hyg. 2002 Nov; 67(5):497-503.AJ

Abstract

We compared the performance of 2 commercially available dipstick assays, 2 enzyme-linked immunosorbent assays (ELISAs), and an indirect immunofluorescent antibody (IFA) assay for the diagnosis of scrub typhus, using the indirect immunoperoxidase (IIP) test as the reference standard. The dipstick assays were the Integrated Diagnostics (Baltimore, MD) Dip-S-Ticks Scrub Recombinant (r56) dipstick test (INDX assay) and the PanBio (Brisbane, Australia) Scrub Typhus IgM and IgG Rapid Immunochromatographic test (PanBio assay). One of the ELISAs used pooled cell lysates of Karp, Kato, and Gilliam strain Orientia tsutsugamushi as antigen (pooled-antigen ELISA), and the other used a recombinant r56 protein as the antigen (recombinant ELISA). With a panel of 123 positive and 227 negative sera, sensitivity and specificity of the assays were as follows: INDX assay, IgG, 60% and 95%, IgM, 60% and 97%; PanBio assay, IgG, 94% and 96%, IgM, 83% and 93%; IFA (1:400 cutoff), IgG, 91% and 96%, IgM, 85% and 98%; pooled-antigen ELISA, IgG (1:1600 cutoff), 97% and 89%, IgM (1:400 cutoff), 94% and 91%; recombinant ELISA, IgG (1:1600 cutoff), 97% and 92%, IgM (1:400 cutoff), 93% and 94%. Because of its excellent performance and use of a standardized, commercially available antigen, the recombinant ELISA is suitable for use in a diagnostic laboratory, where it may be able to replace the IFA and IIP assays. In contrast, the PanBio dipstick assay was easy to perform and did not require sophisticated equipment, making it suitable for use in rural areas where more sophisticated diagnostic tests such as the ELISA and IFA may not be available.

Authors+Show Affiliations

Coleman RE
Department of Entomology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. Russell.coleman@det.amedd.army.mil
Sangkasuwan V
No affiliation info available
Suwanabun N
No affiliation info available
Eamsila C
No affiliation info available
Mungviriya S
No affiliation info available
Devine P
No affiliation info available
Richards AL
No affiliation info available
Rowland D
No affiliation info available
Ching WM
No affiliation info available
Sattabongkot J
No affiliation info available
Lerdthusnee K
No affiliation info available

MeSH

Antibodies, BacterialEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectHumansImmunoglobulin GImmunoglobulin MMaleOrientia tsutsugamushiReproducibility of ResultsRural HealthScrub TyphusSensitivity and SpecificitySerologic TestsThailand

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12479551

Citation

Coleman, Russell E., et al. "Comparative Evaluation of Selected Diagnostic Assays for the Detection of IgG and IgM Antibody to Orientia Tsutsugamushi in Thailand." The American Journal of Tropical Medicine and Hygiene, vol. 67, no. 5, 2002, pp. 497-503.
Coleman RE, Sangkasuwan V, Suwanabun N, et al. Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. Am J Trop Med Hyg. 2002;67(5):497-503.
Coleman, R. E., Sangkasuwan, V., Suwanabun, N., Eamsila, C., Mungviriya, S., Devine, P., Richards, A. L., Rowland, D., Ching, W. M., Sattabongkot, J., & Lerdthusnee, K. (2002). Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. The American Journal of Tropical Medicine and Hygiene, 67(5), 497-503.
Coleman RE, et al. Comparative Evaluation of Selected Diagnostic Assays for the Detection of IgG and IgM Antibody to Orientia Tsutsugamushi in Thailand. Am J Trop Med Hyg. 2002;67(5):497-503. PubMed PMID: 12479551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. AU - Coleman,Russell E, AU - Sangkasuwan,Vichai, AU - Suwanabun,Nantavadee, AU - Eamsila,Chirapa, AU - Mungviriya,Siriporn, AU - Devine,Peter, AU - Richards,Allen L, AU - Rowland,Denise, AU - Ching,Wei-Mei, AU - Sattabongkot,Jetsumon, AU - Lerdthusnee,Kriangkrai, PY - 2002/12/14/pubmed PY - 2003/1/8/medline PY - 2002/12/14/entrez SP - 497 EP - 503 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 67 IS - 5 N2 - We compared the performance of 2 commercially available dipstick assays, 2 enzyme-linked immunosorbent assays (ELISAs), and an indirect immunofluorescent antibody (IFA) assay for the diagnosis of scrub typhus, using the indirect immunoperoxidase (IIP) test as the reference standard. The dipstick assays were the Integrated Diagnostics (Baltimore, MD) Dip-S-Ticks Scrub Recombinant (r56) dipstick test (INDX assay) and the PanBio (Brisbane, Australia) Scrub Typhus IgM and IgG Rapid Immunochromatographic test (PanBio assay). One of the ELISAs used pooled cell lysates of Karp, Kato, and Gilliam strain Orientia tsutsugamushi as antigen (pooled-antigen ELISA), and the other used a recombinant r56 protein as the antigen (recombinant ELISA). With a panel of 123 positive and 227 negative sera, sensitivity and specificity of the assays were as follows: INDX assay, IgG, 60% and 95%, IgM, 60% and 97%; PanBio assay, IgG, 94% and 96%, IgM, 83% and 93%; IFA (1:400 cutoff), IgG, 91% and 96%, IgM, 85% and 98%; pooled-antigen ELISA, IgG (1:1600 cutoff), 97% and 89%, IgM (1:400 cutoff), 94% and 91%; recombinant ELISA, IgG (1:1600 cutoff), 97% and 92%, IgM (1:400 cutoff), 93% and 94%. Because of its excellent performance and use of a standardized, commercially available antigen, the recombinant ELISA is suitable for use in a diagnostic laboratory, where it may be able to replace the IFA and IIP assays. In contrast, the PanBio dipstick assay was easy to perform and did not require sophisticated equipment, making it suitable for use in rural areas where more sophisticated diagnostic tests such as the ELISA and IFA may not be available. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/12479551/Comparative_evaluation_of_selected_diagnostic_assays_for_the_detection_of_IgG_and_IgM_antibody_to_Orientia_tsutsugamushi_in_Thailand_ DB - PRIME DP - Unbound Medicine ER -