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alpha-Ketoisocaproic acid regulates phosphorylation of intermediate filaments in postnatal rat cortical slices through ionotropic glutamatergic receptors.
Brain Res Dev Brain Res. 2002 Dec 15; 139(2):267-76.BR

Abstract

In this study we investigated the effects of alpha-ketoisocaproic acid (KIC), the main keto acid accumulating in the inherited neurometabolic disorder maple syrup urine disease (MSUD), on the in vitro incorporation of 32P into intermediate filament (IF) proteins from cerebral cortex of rats during development. KIC decreased the in vitro incorporation of 32P into the IF proteins studied up to day 12, had no effect on day 15, and increased this phosphorylation in cortical slices of 17- and 21-day-old rats. A similar effect on IF phosphorylation was achieved along development by incubating cortical slices with glutamate. Furthermore, the altered phosphorylation caused by the presence of KIC in the incubation medium was mediated by the ionotropic receptors NMDA, AMPA and kainate up to day 12 and by NMDA and AMPA in tissue slices from 17- and 21-day-old rats. The results suggest that alterations of IF phosphorylation may be associated with the neuropathology of MSUD.

Authors+Show Affiliations

Universidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da Saúde, Departamento de Bioquímica, Rua Ramiro Barcelos 2600 anexo, 90035-003, Porto Alegre, RS, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12480141

Citation

Funchal, Cláudia, et al. "Alpha-Ketoisocaproic Acid Regulates Phosphorylation of Intermediate Filaments in Postnatal Rat Cortical Slices Through Ionotropic Glutamatergic Receptors." Brain Research. Developmental Brain Research, vol. 139, no. 2, 2002, pp. 267-76.
Funchal C, de Lima Pelaez P, Loureiro SO, et al. Alpha-Ketoisocaproic acid regulates phosphorylation of intermediate filaments in postnatal rat cortical slices through ionotropic glutamatergic receptors. Brain Res Dev Brain Res. 2002;139(2):267-76.
Funchal, C., de Lima Pelaez, P., Loureiro, S. O., Vivian, L., Dall Bello Pessutto, F., de Almeida, L. M., Tchernin Wofchuk, S., Wajner, M., & Pessoa Pureur, R. (2002). Alpha-Ketoisocaproic acid regulates phosphorylation of intermediate filaments in postnatal rat cortical slices through ionotropic glutamatergic receptors. Brain Research. Developmental Brain Research, 139(2), 267-76.
Funchal C, et al. Alpha-Ketoisocaproic Acid Regulates Phosphorylation of Intermediate Filaments in Postnatal Rat Cortical Slices Through Ionotropic Glutamatergic Receptors. Brain Res Dev Brain Res. 2002 Dec 15;139(2):267-76. PubMed PMID: 12480141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - alpha-Ketoisocaproic acid regulates phosphorylation of intermediate filaments in postnatal rat cortical slices through ionotropic glutamatergic receptors. AU - Funchal,Cláudia, AU - de Lima Pelaez,Priscila, AU - Loureiro,Samanta Oliveira, AU - Vivian,Lilian, AU - Dall Bello Pessutto,Franciele, AU - de Almeida,Lúcia Maria Vieira, AU - Tchernin Wofchuk,Susana, AU - Wajner,Moacir, AU - Pessoa Pureur,Regina, PY - 2002/12/14/pubmed PY - 2003/4/4/medline PY - 2002/12/14/entrez SP - 267 EP - 76 JF - Brain research. Developmental brain research JO - Brain Res Dev Brain Res VL - 139 IS - 2 N2 - In this study we investigated the effects of alpha-ketoisocaproic acid (KIC), the main keto acid accumulating in the inherited neurometabolic disorder maple syrup urine disease (MSUD), on the in vitro incorporation of 32P into intermediate filament (IF) proteins from cerebral cortex of rats during development. KIC decreased the in vitro incorporation of 32P into the IF proteins studied up to day 12, had no effect on day 15, and increased this phosphorylation in cortical slices of 17- and 21-day-old rats. A similar effect on IF phosphorylation was achieved along development by incubating cortical slices with glutamate. Furthermore, the altered phosphorylation caused by the presence of KIC in the incubation medium was mediated by the ionotropic receptors NMDA, AMPA and kainate up to day 12 and by NMDA and AMPA in tissue slices from 17- and 21-day-old rats. The results suggest that alterations of IF phosphorylation may be associated with the neuropathology of MSUD. SN - 0165-3806 UR - https://www.unboundmedicine.com/medline/citation/12480141/alpha_Ketoisocaproic_acid_regulates_phosphorylation_of_intermediate_filaments_in_postnatal_rat_cortical_slices_through_ionotropic_glutamatergic_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165380602005783 DB - PRIME DP - Unbound Medicine ER -