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Podocyte-specific expression of cre recombinase in transgenic mice.
Genesis. 2003 Jan; 35(1):39-42.G

Abstract

We report a transgenic mouse line that expresses Cre recombinase exclusively in podocytes. Twenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. Previously, this fragment was shown to drive beta-galactosidase (beta-gal) expression exclusively in podocytes of transgenic mice. For analysis, founder mice were bred with ROSA26 mice, a reporter line that expresses beta-gal in cells that undergo Cre recombination. Eight of 24 founder lines were found to express beta-gal exclusively in the kidney. Histological analysis of the kidneys showed that beta-gal expression was confined to podocytes. Cre recombination occurred during the capillary loop stage in glomerular development. No evidence for Cre recombination was detected in any of 14 other tissues examined.

Authors+Show Affiliations

Division of Nephrology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12481297

Citation

Moeller, Marcus J., et al. "Podocyte-specific Expression of Cre Recombinase in Transgenic Mice." Genesis (New York, N.Y. : 2000), vol. 35, no. 1, 2003, pp. 39-42.
Moeller MJ, Sanden SK, Soofi A, et al. Podocyte-specific expression of cre recombinase in transgenic mice. Genesis. 2003;35(1):39-42.
Moeller, M. J., Sanden, S. K., Soofi, A., Wiggins, R. C., & Holzman, L. B. (2003). Podocyte-specific expression of cre recombinase in transgenic mice. Genesis (New York, N.Y. : 2000), 35(1), 39-42.
Moeller MJ, et al. Podocyte-specific Expression of Cre Recombinase in Transgenic Mice. Genesis. 2003;35(1):39-42. PubMed PMID: 12481297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Podocyte-specific expression of cre recombinase in transgenic mice. AU - Moeller,Marcus J, AU - Sanden,Silja K, AU - Soofi,Abdulsalam, AU - Wiggins,Roger C, AU - Holzman,Lawrence B, PY - 2002/12/14/pubmed PY - 2003/8/7/medline PY - 2002/12/14/entrez SP - 39 EP - 42 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 35 IS - 1 N2 - We report a transgenic mouse line that expresses Cre recombinase exclusively in podocytes. Twenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. Previously, this fragment was shown to drive beta-galactosidase (beta-gal) expression exclusively in podocytes of transgenic mice. For analysis, founder mice were bred with ROSA26 mice, a reporter line that expresses beta-gal in cells that undergo Cre recombination. Eight of 24 founder lines were found to express beta-gal exclusively in the kidney. Histological analysis of the kidneys showed that beta-gal expression was confined to podocytes. Cre recombination occurred during the capillary loop stage in glomerular development. No evidence for Cre recombination was detected in any of 14 other tissues examined. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/12481297/Podocyte_specific_expression_of_cre_recombinase_in_transgenic_mice_ L2 - https://doi.org/10.1002/gene.10164 DB - PRIME DP - Unbound Medicine ER -