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Platelet serotonin measures in migraine.
Headache. 2002 Jul-Aug; 42(7):588-95.H

Abstract

OBJECTIVE AND BACKGROUND

Serotonergic mechanisms play an important role in the pathogenesis of headache. To search for potential indicators of altered serotonin homeostasis in migraine, we have investigated three parameters of the platelet serotonin (5HT) system, platelet serotonin level (PSL), platelet serotonin uptake (PSU), and monoamine oxidase (MAO-B) activity, in a group of 55 patients with migraine and in 81 healthy controls.

METHODS

After platelet separation, PSL was determined fluorimetrically; PSU was measured by incubating aliquots of platelet-rich plasma with six concentrations of 14C-5-HT for 60 seconds at 37 degrees C, followed by vacuum filtration; platelet MAO-B activity (toward kynuramine as a substrate) was determined fluorimetrically.

RESULTS

Values of the investigated measures, in patients versus controls, amounted to (mean +/- SD) 608 +/- 166 vs. 591 +/- 184 ng/10(9) platelets for PSL, 139 +/- 25 vs. 142 +/- 25 pmol 5HT/10(8) platelets/minute for Vmax of PSU, 376 +/- 62 vs. 404 +/- 72 nM for Km of PSU, and 15.8 +/- 5.1 vs. 14.3 +/- 5.7 nmol product/10(8) platelets/60 minutes for velocity of MAO-B. Mentioned parameters did not show statistical differences between patients and controls, with exception of a small difference in Km of PSU, reaching significance (P<0.01). After subgrouping of patients according to diagnosis (migraine with aura, migraine without aura, and migraine attack) and gender, no differences retained significance.

CONCLUSIONS

Our results indicate the absence of a measurable disturbance in 5HT homeostasis in migraine, as shown by platelet 5HT parameters, and they question the suitability of the use of mentioned blood elements in this regard.

Authors+Show Affiliations

Laboratory of Neurochemistry and Molecular Neurobiology, Rudjer Boskovic Institute, Bijenicka 54, Zagreb, Croatia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12482210

Citation

Jernej, Branimir, et al. "Platelet Serotonin Measures in Migraine." Headache, vol. 42, no. 7, 2002, pp. 588-95.
Jernej B, Vladić A, Cicin-Sain L, et al. Platelet serotonin measures in migraine. Headache. 2002;42(7):588-95.
Jernej, B., Vladić, A., Cicin-Sain, L., Hranilović, D., Banović, M., Balija, M., Bilić, E., Sucić, Z., Vukadin, S., & Grgicević, D. (2002). Platelet serotonin measures in migraine. Headache, 42(7), 588-95.
Jernej B, et al. Platelet Serotonin Measures in Migraine. Headache. 2002 Jul-Aug;42(7):588-95. PubMed PMID: 12482210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platelet serotonin measures in migraine. AU - Jernej,Branimir, AU - Vladić,Anton, AU - Cicin-Sain,Lipa, AU - Hranilović,Dubravka, AU - Banović,Miroslav, AU - Balija,Melita, AU - Bilić,Ervina, AU - Sucić,Zvonimir, AU - Vukadin,Stjepan, AU - Grgicević,Damir, PY - 2002/12/17/pubmed PY - 2003/2/13/medline PY - 2002/12/17/entrez SP - 588 EP - 95 JF - Headache JO - Headache VL - 42 IS - 7 N2 - OBJECTIVE AND BACKGROUND: Serotonergic mechanisms play an important role in the pathogenesis of headache. To search for potential indicators of altered serotonin homeostasis in migraine, we have investigated three parameters of the platelet serotonin (5HT) system, platelet serotonin level (PSL), platelet serotonin uptake (PSU), and monoamine oxidase (MAO-B) activity, in a group of 55 patients with migraine and in 81 healthy controls. METHODS: After platelet separation, PSL was determined fluorimetrically; PSU was measured by incubating aliquots of platelet-rich plasma with six concentrations of 14C-5-HT for 60 seconds at 37 degrees C, followed by vacuum filtration; platelet MAO-B activity (toward kynuramine as a substrate) was determined fluorimetrically. RESULTS: Values of the investigated measures, in patients versus controls, amounted to (mean +/- SD) 608 +/- 166 vs. 591 +/- 184 ng/10(9) platelets for PSL, 139 +/- 25 vs. 142 +/- 25 pmol 5HT/10(8) platelets/minute for Vmax of PSU, 376 +/- 62 vs. 404 +/- 72 nM for Km of PSU, and 15.8 +/- 5.1 vs. 14.3 +/- 5.7 nmol product/10(8) platelets/60 minutes for velocity of MAO-B. Mentioned parameters did not show statistical differences between patients and controls, with exception of a small difference in Km of PSU, reaching significance (P<0.01). After subgrouping of patients according to diagnosis (migraine with aura, migraine without aura, and migraine attack) and gender, no differences retained significance. CONCLUSIONS: Our results indicate the absence of a measurable disturbance in 5HT homeostasis in migraine, as shown by platelet 5HT parameters, and they question the suitability of the use of mentioned blood elements in this regard. SN - 0017-8748 UR - https://www.unboundmedicine.com/medline/citation/12482210/Platelet_serotonin_measures_in_migraine_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0017-8748&amp;date=2002&amp;volume=42&amp;issue=7&amp;spage=588 DB - PRIME DP - Unbound Medicine ER -